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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtacgcccaaaacaaacaacttcaatgctatccaaaaagtaccacgccacgtcatatgtgttaggaagtaggaacagaacatctgcagaacagctgtgttatttggaagatttctttttaagtcctccatttcatgttatatcactaaaatttgagatgatgtgcgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCAGAGGATCCATTGTTTCTTCTATATACAAGTGGTAGCACAGGAAAGCCAA

Basic information

species Oryza sativa
transcript LOC_Os04g33190.1
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os04g33190.1 (Oryza sativa), 3'ss of exon 6
lower sequence: GRMZM2G157269_T01 (Zea mays), 3'ss of exon 6
gtacgcccaaaacaaacaacttcaatgctatccaaaaagtaccacgccacgtcatatgtgttaggaagtaggaacagaacatctgcagaacagctgtgttatttggaagatttctttttaagtcctccatttcatgttatatcactaa--aatttg-----agatgatgtgcgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCAGAGGATCCATTGTTTCTTCTATATACAAGTGGTAGCACAGGAAAGCCAA
                            ||| | || |  |  |     |  ||||  |  |     |   | || ||   ||||||||||  |||||  |||   || ||  | ||  ||  ||| |  |    | | |||||||||  |||||        ||||     |      |||||||||||||  |||| ||||| ||||| |||||||||||||| ||||||||||||||| |||||||| | || |||||||| ||||||||||||||||
---------------------------gtatgccaagatca--aaattgcaacatactttct-----gcgtgcacgga--ctctgcagaacttctgtg--attcataaaataaccttccaaacccttctgtatcagctgtatcactaatcaatttatttctgaatgaacgatgatgaatagGATGTTGTGCCTGATTTCCCAACTAAATGCGATGTGGAATGGGTTGATGCAGAGGATCCACTGTTTCTTTTGTACACAAGTGGCAGCACAGGAAAGCCAA

upper sequence: LOC_Os04g33190.1 (Oryza sativa), 3'ss of exon 6
lower sequence: GLYMA19G28300.1 (Glycine max), 3'ss of exon 6
gtacgcccaaaacaaacaacttcaatgctatccaaaaagtaccacgccacgtcatatgtgttaggaagtaggaacagaacatctgcagaacagctgtgttatttggaagatttctttttaagtcctccatttcatgttatatcactaaaatttgagatgatgtgcgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCAGAGGATCCATTGTTTCTTCTATATACAAGTGGTAGCACAGGAAAGCCAA
                                                             ||  || || |     ||  |     |   | || |  |  |   || ||| |    |   || |  |     | || |   || |   |           ||  |     |||||||  | ||  | | |||||| |  |||    |||| ||||| ||||||||||||||| | ||||| || ||||||||||| ||||||||||| || |
-------------------------------------------------------------tattaaatatgttttcaatgttcatggt-tacctataattatgataaaattccgcact---tctttaaacctgttttttgctaccatgttac---------tgttttttgtagGATGTCATTCCTCAATATCCAACTACTTGTCCGTTGGAGTGGGTTGATGCAGAGGATCCACTTTTTCTACTCTATACAAGTGGGAGCACAGGAAAACCTA

upper sequence: LOC_Os04g33190.1 (Oryza sativa), 3'ss of exon 6
lower sequence: GLYMA16G04910.1 (Glycine max), 3'ss of exon 6
gtacgcccaaaacaaacaacttcaatgctatccaaaaagtaccacgccacgtcatatgtgttaggaagtaggaacagaacatctgcagaacagctgtg-ttatttggaagatttctttttaagtcctccatttcatgttatatcactaaaatttgagatgatgtgcgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCAGAGGATCCATTGTTTCTTCTATATACAAGTGGTAGCACAGGAAAGCCAA
                                                                      ||  |  || || |  | ||      || |||  |  ||   || |  | || | || || ||| |  || | |  |      |   ||| |   | |     |||||||  | |   | | |||||| |  |||   ||||| ||||| ||||||||||||||| | ||||| || ||||||||||| || |||||||| ||||
----------------------------------------------------------------------ggtgctaaatatgtttataatgttcatggttacctataa---ttatgataaaattctacacttctt--taaacctttttttgctatcatgttactctttttgtagGATGTCATTCATCAATATCCAACTACTTGTCCAGTGGAGTGGGTTGATGCAGAGGATCCACTTTTTCTACTCTATACAAGTGGGAGTACAGGAAAACCAA

upper sequence: LOC_Os04g33190.1 (Oryza sativa), 3'ss of exon 6
lower sequence: Vv14s0068g00640.t01 (Vitis vinifera), 3'ss of exon 6
gtacgcccaaaacaaacaacttcaatgctatccaaaaagtaccacgccacgtcatatgtgttaggaagtaggaacagaacatctgcagaacagctgtgttatttggaagatttctttttaagtcctccatttcatgttatatcactaaaatttgagatgatgtgcgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCAGAGGATCCATTGTTTCTTCTATATACAAGTGGTAGCACAGGAAAGCCAA
                                                                 |||||   | |   ||  ||    | |    ||  | ||| | ||  |    ||||            ||  ||| |     || |      | |     |||  |||||||||||| || || | |||||| |  ||||||||||| ||||| |||||||| |||||| | ||||| || ||||||||||| ||||| |||||||| |
-----------------------------------------------------------------aagtatagaaatggcaattg----ataatcttg--aattgaaggaagtgccgttaa---taaagagcaatagtattttgaggaattgatgattaacaactgtcttccagGATGTTGTTCCTAAATATCCAACTACTTGTGATGTGGAGTGGGTTGATGCAGAAGATCCACTTTTTCTGCTCTATACAAGTGGGAGCACGGGAAAGCCCA

upper sequence: LOC_Os04g33190.1 (Oryza sativa), 3'ss of exon 6
lower sequence: PP1S61_296V6.1 (Physcomitrella patens), 3'ss of exon 5
gtacgcccaaaacaaacaacttcaatgctatccaaaaagtaccacgccacgtcatatgtgttaggaagtaggaacagaacatctgcagaacagctgtgt-tatttggaagatttctttttaagtcctccatttcatgttatatcactaaaatttgagatgatgtgcgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCAGAGGATCCATTGTTTCTTCTATATACAAGTGGTAGCACAGGAAAGCCAA
                                                           || |         ||  |  | |  |    |    | | | ||  |  |  | | |  |   |  || ||   | ||  |   ||||  | |||     ||  ||        |||||||| |||||   |  | ||| | ||||||| ||||| ||||| |||||||||||| | ||||| ||  |||| |||||||| ||||| || ||||| |
-----------------------------------------------------------gtaattcctcgtcaatgg--ctttcgtgctatgattttctatacctccaccactccactaacaaaccccctcattatcgtgactcac--atattctctgtggggt-ttcattacagGATGTGGTGCCATCTCATTCAAAAGAATGTGAAGTGGAGTGGGTCGATGCAGAGGATACTTTGTTCCTGTTATACACAAGTGGAAGCACTGGGAAGCCCA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|58606874|gb|CK034907.1|CK034907
EST:     TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAG                         GATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATG
genomic: TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATG
EST: gi|29637985|gb|CB642994.1|CB642994
EST:     TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAG                         GATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
genomic: TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
EST: gi|29627341|gb|CB632352.1|CB632352
EST:     TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAG                         GATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
genomic: TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
EST: gi|29629232|gb|CB634243.1|CB634243
EST:     TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAG                         GATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
genomic: TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
EST: gi|29667040|gb|CB663315.1|CB663315
EST:     TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAG                         GATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
genomic: TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
EST: gi|33817601|gb|CF334632.1|CF334632
EST:     TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAG                         GATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
genomic: TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
EST: gi|5701680|gb|AU062515.2|AU062515
EST:     AGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAG                         GATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
genomic: AGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
EST: gi|29641346|gb|CB646353.1|CB646353
EST:     TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAG                         GATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
genomic: TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
EST: gi|29677034|gb|CB673309.1|CB673309
EST:     AGAAGACACAAAATGGACGTCTGGCAGAGACGTTTGGTGGCAG                         GATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
genomic: AGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
EST: gi|29624451|gb|CB629462.1|CB629462
EST:     TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAG                         GATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA
genomic: TGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 74 (upstream exon), 75 (downstream exon)
Score: 30

GCATTTCTTTGACATACGAAAATCAGTCGGCAATGAAGAGAGAAGACACAAAATGGACGTCAGGCAGAGACGTTTGGTGGCAGgtacgcccaa ... cgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCAGAGGATCCATTGTTTCTTCTATATACAAGTGGTAGCACAGGAAAGCCAA




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aagtcctccatttcatgttatatcactaaaatttgagatgatgtgcgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCAGAGGATCCATTGTTTCTTCTATATACAAGTGGTAGCACAGGAAAGCCAA
                       cactaaa  putative branch site (score: 30)
                                             cgtccccc  putative PPT
                 ttatatcactaaaatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtacgcccaaaacaaacaacttcaatgctatccaaaaagtaccacgccacgtcatatgtgttaggaagtaggaacagaacatctgcagaacagctgtgttatttggaagatttctttttaagtcctccatttcatgttatatcactaaaatttgagatgatgtgcgtcccccagGATGTTGTGCCCAATTTTCCAACAAAATGTGATGTGGAATGGGTAGATGCAGAGGATCCATTGTTTCTTCTATATACAAGTGGTAGCACAGGAAAGCCAA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGGAT