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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...agtctggggccatgttcttatggtgtatcggttattacctttggaatatctgcattgacctatacagtcttatgttctttaccactttaccttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGTTCCAACGTGGATCTGAGTGCTTCAGATTTGATCAACCGGCTTTCTTTCC

Basic information

species Oryza sativa
transcript LOC_Os10g42420.1
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os10g42420.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GRMZM2G134045_T02 (Zea mays), 3'ss of exon 5
agtctggggccatgttcttatggtgtatcggttattacctttggaatatctgcattgacctatacagtcttatgttctttaccactttaccttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGTTCCAACGTGGATCTGAGTGCTTCAGATTTGATCAACCGGCTTTCTTTCC
      |  | | ||   |||    ||      || |   |||| |  |  | |      | |  | |||||   || |  | |||| | || | || |||| || |||||||| |||||||||||||||||||| |||||| | || | ||| | ||||||||| ||||||||||||||||||||| |  | || ||||
--gtaagtactacgtcgctatttcatagtcaccataataattggtagtttggtacca---tgttgaatcttacactcctcccaacttcaactcgttgcagGGTTCCTCTGCCATATCAAGTGCTGATCTATTTGGCCATCCAGCAAACAATTCTAGCGTGGATCTCAGTGCTTCAGATTTGATCAACAGAATATCATTCC

upper sequence: LOC_Os10g42420.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GRMZM2G094390_T01 (Zea mays), 3'ss of exon 5
agtctggggccatgttcttatggtgtatcggttattacctttggaatatctgcattgacctatacagtcttatgttctttaccactttaccttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGTTCCAACGTGGATCTGAGTGCTTCAGATTTGATCAACCGGCTTTCTTTCC
       |       |  || | |   |   | ||   |     ||||   |   |  | | |    |||||   || |  | |||||| || | || |||| || ||||| || ||||||||||| |||||||| |||||  | || | ||| | ||||||||| ||||||||||||||||||||| |  | || ||||
-------gtaagcactactacg-tccctatttcatggtcaccataataatcgg--taccatgttggatcttaaactcctcccaactttaactcgttgcagGGTTCCTCTGCGATATCAAGTGCTGACCTATTTGGCCATCCGGCAAACAATTCTAGCGTGGATCTCAGTGCTTCAGATTTGATCAACAGAATCTCGTTCC
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|218493112|gb|FG943279.1|FG943279
EST:     CCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCG                         GGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
genomic: CCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
EST: gi|87022045|gb|CI259117.1|CI259117
EST:     ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCG                         GGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
genomic: ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
EST: gi|88906383|gb|CI375455.1|CI375455
EST:     CAAGTTTCCCTCCAAAAGTTTTCG                         GGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
genomic: CAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
EST: gi|33654042|gb|CF276656.1|CF276656
EST:     ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCG                         GGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
genomic: ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
EST: gi|58610590|gb|CK038302.1|CK038302
EST:     CTCAAGTTTCCCTCCAAAAGTTTTCG                         GGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
genomic: CTCAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
EST: gi|86489006|gb|CI131639.1|CI131639
EST:     TCCCTCCAAAAGTTTTCG                         GGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
genomic: TCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
EST: gi|58695054|gb|CK083741.1|CK083741
EST:     ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTATCG                         GGATCTTCTGACATTTCAAGTGCTGATCTATTTGGTCATC
genomic: ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATC
EST: gi|86250851|gb|CI007714.1|CI007714
EST:     ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCG                         GGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
genomic: ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
EST: gi|86495273|gb|CI137906.1|CI137906
EST:     ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCG                         GGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCCATAGT
genomic: ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
EST: gi|218500046|gb|FG955203.1|FG955203
EST:     ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCG                         GGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
genomic: ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT
EST: gi|86594982|gb|CI258236.1|CI258236
EST:     ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCG                         GGATCTTCTGCCATTTCAAGTGCTGGTCTATTTGGTCATCCAACCAATAGT
genomic: ACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 68 (upstream exon), 69 (downstream exon)
Score: 19

ACAGAAGTTTTCAAATGCAAAGTCAATTTCATCTTCTCAGTTTTTTGGAGACCAGGCCAGTTTTGAAAAGGAGGCTCAAGTTTCCCTCCAAAAGTTTTCGgtaagtattt ... cttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGTTCCAACGTGGATCTGAGTGCTTCAGATTTGATCAACCGGCTTTCTTTCC




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atatctgcattgacctatacagtcttatgttctttaccactttaccttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGTTCCAACGTGGATCTGAGTGCTTCAGATTTGATCAACCGGCTTTCTTTCC
       cattgac  putative branch site (score: 19)
                                       ctttacctt  putative PPT
                        ttatgtt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
agtctggggccatgttcttatggtgtatcggttattacctttggaatatctgcattgacctatacagtcttatgttctttaccactttaccttgctgtagGGATCTTCTGCCATTTCAAGTGCTGATCTATTTGGTCATCCAACCAATAGTTCCAACGTGGATCTGAGTGCTTCAGATTTGATCAACCGGCTTTCTTTCC

- -ctggggc
- - - - - catgttc
- - - - - - - -tcttatg
- - - - - - - - - - - gtgtatc
- - - - - - - - - - - - - - - - - - - - - - - -atctgca