ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtttgttgttttattctctccactagtgtcaaggcaatatatttctttaggttttactcttttgtttggtaaaacagtaagaataatattatgctcgttgcttcagGAACGCCAACAAGCAGAAGAGGAGGCTAAGATGAAGGAAGCTGAACTGATGCGGGGAAACCCCCTGATCAATATTAACAACGCCGGATCCTTCAATGTTA

Basic information

species	Oryza sativa
transcript	LOC_Os06g01700.1
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os06g01700.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GRMZM2G154278_T01 (Zea mays), 3'ss of exon 5

gtttgttgttttattctctccactagtgtcaaggcaatatatttcttta-ggttttactcttttgtttggtaaaacagtaagaataat-attatgctcgttgc--ttcagGAACGCCAACAAGCAGAAGAGGAGGCTAAGATGAAGGAAGCTGAACTGATGCGGGGAAACCCCCTGATCAATATTAACAACGCCGGATCCTTCAATGTTA
                                     | | ||   | |   |||||   ||||||    | | |   | ||    || |  |   | ||| |  ||||||| || ||||||||||| |||||||| ||||||||||| |||||||||||||||||||||||  |||| ||||| |||||  | || ||||||| ||| |
------------------------------------gtttgttgtgtcataattttatgtttttgtacatttagattatgagggacatgacgagaattgttccatttcagGAGCGGCAACAAGCAGAGGAGGAGGCCAAGATGAAGGAGGCTGAACTGATGCGGGGAAACCCTTTGATTAATATCAACAATCCTGGCTCCTTCAGTGTCA

upper sequence: LOC_Os06g01700.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GRMZM2G062076_T01 (Zea mays), 3'ss of exon 5

gtttgttgttttattctctccactagtgtcaaggcaatatatttctttaggttttactcttttgtttggtaaaacagtaagaataatattatgctcgttgcttcagGAACGCCAACAAGCAGAAGAGGAGGCTAAGATGAAGGAAGCTGAACTGATGCGGGGAAACCCCCTGATCAATATTAACAACGCCGGATCCTTCAATGTTA
||||||| |   |||   ||     |||||| |    ||| ||| | ||  | |     ||| | ||          |||  | |||    || ||   | ||||||| || |||||||||||||||||||| ||||||||||| |||||||||||||||||||||||  |||| ||||| ||||| || || ||||||| |||||
gtttgttatc--attgcatctgtctgtgtcatgattttatgtttttgtatatctggaattttggattatggggggcataatgagaat----tgttc--cgtttcagGAGCGGCAACAAGCAGAAGAGGAGGCCAAGATGAAGGAGGCTGAACTGATGCGGGGAAACCCGTTGATTAATATCAACAATGCTGGCTCCTTCAGTGTTA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|88508601|gb|CI436107.1|CI436107

EST:     GAAGAAAACCTTAGAAAG                         GAACGCCAACAAGCAGAAGAGGAGGCTAAGATGAAGGAAGCTGAACTGATG
genomic: GAAGAAAACCTTAGAAAGgtttgttgtt ... gttgcttcagGAACGCCAACAAGCAGAAGAGGAGGCTAAGATGAAGGAAGCTGAACTGATG

EST: gi|88253980|gb|CI352606.1|CI352606

EST:     GAGCTGAAGAAAACCTTAGAAAG                         GAACGCCAACAAGCAGAAGAGGAGGCTAAGATGAAGGAAGCTGAACTGATG
genomic: GAGCTGAAGAAAACCTTAGAAAGgtttgttgtt ... gttgcttcagGAACGCCAACAAGCAGAAGAGGAGGCTAAGATGAAGGAAGCTGAACTGATG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 42 (upstream exon), 75 (downstream exon)
Score: 144

TGATGAGGATGATGACGACGATGATGACACTGAAGCTCTAATGGCAGAGCTTGAAAGAATCAAGAAAGAAAGAGCTGAAGAAAACCTTAGAAAGgtttgttgtt ... gttgcttcagGAACGCCAACAAGCAGAAGAGGAGGCTAAGATGAAGGAAGCTGAACTGATGCGGGGAAACCCCCTGATCAATATTAACAACGCCGGATCCTTCAATGTTA

<

<

<

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttttactcttttgtttggtaaaacagtaagaataatattatgctcgttgcttcagGAACGCCAACAAGCAGAAGAGGAGGCTAAGATGAAGGAAGCTGAACTGATGCGGGGAAACCCCCTGATCAATATTAACAACGCCGGATCCTTCAATGTTA