ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

...ttagacatgtggatgcttgctcctagtgtgaatgctactttatgtttttcttaaaagaagttattatatggcctttcttcacatattttcttcttaacagATTTGACAAGAAGATAAGGTATGCTTCTCGTAAGGCTAGGGCAGATGTGAGAAAAAGGGTCAAAGGACGGTTTGTAAAGGCTGGCGAAGCATATGACTAC

Basic information

species	Oryza sativa
transcript	LOC_Os02g49230.3
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os02g49230.2 (Oryza sativa), 3'ss of exon 6
lower sequence: GRMZM2G167560_T01 (Zea mays), 3'ss of exon 3

--------------------------------------------------------------------------------------ttagacatgtggatg-cttgctcctagtgtgaatgctactttatgtttttcttaaaagaagttattatatggcctttcttcacatattttcttcttaacagATTTGACAAGAAGATAAGGTATGCTTCTCGTAAGGCTAGGGCAGATGTGAGAAAAAGGGTCAAAGGACGGTTTGTAAAGGCTGGCGAAGCATATGACTAC
                                                                                        |  | | ||  |  |||| | ||||| |  ||  | |   || ||  |   | |||  | |  |  |       | |||||  ||||     |  |||| |||||||||||||| || ||||||||||| |||||||||||||| ||||| || |||||||| ||||||||| |||||||||||||||| |||||||| 
gtaagcacgtttaaatgtctgcttgaggttgttctgcgcttaacattcatagatcgtggctatctttttaaggcaaaaaaatgtctccaaccttctgtgtctcttgtttctagtttcgataatgc---attttccttgcagaagtcgctgattcacctttccttttcacgaatttgg---tcgacagGTTTGACAAGAAGATCAGATATGCTTCTCGCAAGGCTAGGGCAGACGTGAGGAAGAGGGTCAAGGGACGGTTTATAAAGGCTGGCGAAGCGTATGACTAT

upper sequence: LOC_Os02g49230.2 (Oryza sativa), 3'ss of exon 6
lower sequence: GRMZM2G075562_T02 (Zea mays), 3'ss of exon 3

-------------------------------------------------ttagacatgtggatgcttgctcctagtgtgaatgct----actttatgtttttcttaaaagaagttattatatggcctt--tcttcacatattttcttc----------ttaacagATTTGACAAGAAGATAAGGTATGCTTCTCGTAAGGCTAGGGCAGATGTGAGAAAAAGGGTCAAAGGACGGTTTGTAAAGGCTGGCGAAGCATATGACTAC
                                                 | | | |       |||  ||  |   |  |||||     || || ||  | |||       ||||   |||  |  ||   |||  | | | || | |             |||| |||||||||||||| || ||||||||||| ||||||||||| || ||||| ||  ||||||| ||||||||  ||||||| || ||||| || ||||| 
gtaagcacggctaaatgtctttttgtggttatttttctgcgcctaacattcatatagaccatggctatctttttaagcaaatgcctcccaccttctgggtctctcgtttctagttccaatactgtattgaccttttcttttcttttccacgaatttggccgacagGTTTGACAAGAAGATCAGATATGCTTCTCGCAAGGCTAGGGCGGACGTGAGGAAGCGGGTCAAGGGACGGTTCATAAAGGCCGGTGAAGCGTACGACTAT

upper sequence: LOC_Os02g49230.2 (Oryza sativa), 3'ss of exon 6
lower sequence: Vv00s0194g00070.t01 (Vitis vinifera), 3'ss of exon 4

--ttagacatgtggatgcttgctcctagtgtgaatgctactttatgtttttcttaaaagaagttattatatggcctttcttcacatattttcttcttaacagATTTGACAAGAAGATAAGGTATGCTTCTCGTAAGGCTAGGGCAGATGTGAGAAAAAGGGTCAAAGGACGGTTTGTAAAGGCTGGCGAAGCATATGACTAC
  | | | | | | | | ||  |       |  ||   | |  |  ||||  | |  | ||  || | | |   |||| ||||   | |  ||| | ||||||||||| ||||| ||||| ||||| ||||| || || ||||||||||| || ||  | || || || || ||||| ||||||||||| || ||||| |||
actaaaataagggtaggatttttatggaatttgattagaatgaaaattttcataactaaaaaataattttttttcttt-ttcat-caatgacttatgaacagATTTGAGAAGAAAATAAGATATGCCTCTCGCAAAGCAAGGGCAGATGTCAGGAAGCGTGTGAAGGGGCGATTTGTTAAGGCTGGCGATGCTTATGATTAC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|700150|gb|D46441.1|D46441

EST:     AGCAGAGATAATGCTCTTACACGGTACAAGGAGAAGAAGAAGAGAAGNAA                         ATTTGACAAGNAGGTAAGGTATGCTTCTCGTAAGGCTAGGGCAGATGTGAG
genomic: AGCAGAGATAATGCTCTTACACGGTACAAGGAGAAGAAGAAGAGAAGAAAgtaagtgcat ... ttcttaacagATTTGACAAGAAGATAAGGTATGCTTCTCGTAAGGCTAGGGCAGATGTGAG

EST: gi|33815340|gb|CF333525.1|CF333525

EST:     AGCAGAGATAATGCTCTTACACGGTACAAGGAGGAGAAGAAGAGAAGAAA                         ATTTGACAAGAAGATAAGGTATGCTTCTCGTAAGGCTAGGGCAGATGTGAG
genomic: AGCAGAGATAATGCTCTTACACGGTACAAGGAGAAGAAGAAGAGAAGAAAgtaagtgcat ... ttcttaacagATTTGACAAGAAGATAAGGTATGCTTCTCGTAAGGCTAGGGCAGATGTGAG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 49 (upstream exon), 70 (downstream exon)
Score: 10

GTGAGCCACCCTGGCATCCTCCTGGTCCTGAAAGTTCATCTGCTGGAGGCAGCAGAGATAATGCTCTTACACGGTACAAGGAGAAGAAGAAGAGAAGAAAgtaagtgcat ... ttcttaacagATTTGACAAGAAGATAAGGTATGCTTCTCGTAAGGCTAGGGCAGATGTGAGAAAAAGGGTCAAAGGACGGTTTGTAAAGGCTGGCGAAGCATATGACTAC

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atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttttcttaaaagaagttattatatggcctttcttcacatattttcttcttaacagATTTGACAAGAAGATAAGGTATGCTTCTCGTAAGGCTAGGGCAGATGTGAGAAAAAGGGTCAAAGGACGGTTTGTAAAGGCTGGCGAAGCATATGACTAC