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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tattctgtagcatctcatgttcatactgtgttttcccctttatatgcttgtctatctgtctagtttagaggctatttgatgtgacccttttgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGACTGGAATAGAATACAAGAAAGTGGCAAAGATGCCGAACTTTTATACGGCC

Basic information

species Oryza sativa
transcript LOC_Os01g08200.1
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os01g08200.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GRMZM2G107696_T02 (Zea mays), 3'ss of exon 9
tattctgtagcatctcatgt-tcatactgtgttttccc--ctttatatgcttgtctatctgtctagtttagaggctatttgatgtgacccttttgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGACTGGAATAGAATACAAGAAAGTGGCAAAGATGCCGAACTTTTATACGGCC
   | |||  || || |||  | |||| || |||| ||  ||| | |||    |  |      |   |    ||| |  |  | |     |||  |  ||||||| ||||||||||||||||| || || ||||||   |||||||||||||||||||||||||||||||||||||| ||||| || || ||| | |  || |
---tatgttacagctgatgcataatacagtttttttcctgcttgacatgtacatagaggcaattttatgcttggccacattgtttataaatttgttcttgcagACTGAGATGACTACTGCTGAGAGGGAACTTGATGCTAACACAAATTATGACTGGAATAGAATACAAGAAAGTGGAAAAGACGCTGAGCTTCTGTTTGGAC

upper sequence: LOC_Os01g08200.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA12G22240.1 (Glycine max), 3'ss of exon 5
--------------------------------------------------------------------------------------tattctgtagcatctcatgttcatactgtgttttcccctttatatgcttgtctatctgtctagtttagaggctatttgatgtgacccttttgttgtg--cagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGACTGGAATAGAATACAAGAAAGTGGCAAAGATGCCGAACTTTTATACGGCC
                                                                                      || ||         |   ||  | | |||  ||     |   ||   ||   ||||  |  | | |  |   | ||  |   | ||  ||| || |   ||||| || ||||| |||||||||||||| |||||||| || || |||| ||| |||||| |||| |||||||||||  |||| |  || |   | |  || |
gttaattatttatcttgaaatgatacttttcacgcacaataaaaaatatattaattattaatcttgagtataaacttttgtcttgttactccaacaggcatggcgta-agagtgtacttaaaattaattaaatttaaatatcacttgactcttaatatt-ttaaactggtccaatttcttatattcagACTGAAATGACAACTGCTGAAAGAGAACTTGATCAAAATACGAATTTTGATTGGAATCGAATCCAAGAAAGTGGACAAGAAGTAGATCCAATTTTTGGAC

upper sequence: LOC_Os01g08200.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA06G39770.1 (Glycine max), 3'ss of exon 5
-------------------------------------------------------------------------------tattctgtagcatct-----catgttcatactgtgttttccccttta--tatgcttgtctatctgtctagtttagaggctatttgatgtgacccttttgt-tgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGACTGGAATAGAATACAAGAAAGTGGCAAAGATGCCGAACTTTTATACGGCC
                                                                               | |  |||  |  |      ||||     |  | ||  |  |  |||  ||   ||   ||||  |  | | |  |   | |   |||   | ||||  | | | ||||| || ||||| |||||||||||||| ||||||||||| ||||||| ||| |||||| |||| |||||||||||  |||| |  || |   | |  || |
gttaattatttatcttgaaatgatacttttcacacacacaatcaaactatattaattatttgtcttgagaataaacttttgtcttgttactccaacaggcatggcataagagcgtacttgcaattaattaaatttaaatatcacttgactcttaatattttaaaatggtccactttcttatattcagACTGAAATGACAACTGCTGAAAGAGAACTTGATCACAATACAAATTTTGATTGGAATCGAATCCAAGAAAGTGGACAAGAAGTAGATCCAATTTTTGGAC

upper sequence: LOC_Os01g08200.1 (Oryza sativa), 3'ss of exon 5
lower sequence: AT3G20630.1 (Arabidopsis thaliana), 3'ss of exon 5
tattctgtagcatctc-atgttcatactgtgttttcccctttatatgcttgtct--atctgtctagtttagaggctatttga-tgtgacccttttgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGACTGGAATAGAATACAAGAAAGTGGCAAAGATGCCGAACTTTTATACGGCC
   | ||||    ||  ||  |||| || || |       |   ||||| || |  |||||  || | |  |   | ||  | |||   |      ||   ||||| || ||||| |||||||||||||| |||||||| || ||||||| ||| |||||  | |||||||| ||||| |||||    |  |   | |  || |
---tttgtactgccttgatcatcat-ctttgctaaagatatcccatgctggttttaatctgaataatgttaactttcttgaaatgtttgctacaattttcacagACTGAAATGACAACTGCTGAAAGAGAACTTGATCAAAATACAAATTTTGATTGGAACCGTATACAAGAGAGTGGAAAAGAATTGGTGCCAGTTTTTGGAC

upper sequence: LOC_Os01g08200.1 (Oryza sativa), 3'ss of exon 5
lower sequence: Vv19s0014g03920.t01 (Vitis vinifera), 3'ss of exon 5
----tattctgtagcatctcat-gttcatactgtgttttcccctttatatgcttgtctatctgtctagtttagaggctatttgatgtgacccttttgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGACTGGAATAGAATACAAGAAAGTGGCAAAGATGCCGAACTTTTATACGGCC
    | || | |   |  | || |||  | ||      ||  | || | | |||   | ||  |  |  |||        |  || |     || |  | | ||||| || ||||||||||||||||| || ||||| || || || |||| ||| |||||  |||| |||||||||||  |||| |  ||||   |||  || |
ttttttttttttgataaatgatagttgttgcttgagaatcaacattgtttacttacatttcattaaaccttaag-----ctcaatatacattttcttgtttccagACTGAAATGACTACTGCTGAAAGGGAACTTGACCAGAATACCAATTTTGATTGGAACCGAATTCAAGAAAGTGGACAAGAAGTTGAACCACTATTTGGAC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|58606193|gb|CK034226.1|CK034226
EST:     TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAG                         ACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
genomic: TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAGgtgagtagta ... tgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
EST: gi|701567|gb|D47858.1|D47858
EST:     TAGCGCAG-ATTTGTCACATTTTGGTATTGATTTNTCTTCACTCCAAAAG                         ACCGAGATGACTACTGCTGAA-GAGAGCTTTATCACAACACAAATTAT
genomic: TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAGgtgagtagta ... tgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTAT
EST: gi|33677994|gb|CF306233.1|CF306233
EST:     TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAG                         ACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
genomic: TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAGgtgagtagta ... tgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
EST: gi|58663886|gb|CK052572.1|CK052572
EST:     TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAG                         ACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
genomic: TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAGgtgagtagta ... tgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
EST: gi|29622184|gb|CB627195.1|CB627195
EST:     TATCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAG                         ACCGAGATGACTACTGCTGAAAGATAGCTTGATCACAACACAAATTATGAC
genomic: TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAGgtgagtagta ... tgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
EST: gi|58693785|gb|CK082472.1|CK082472
EST:     TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAG                         ACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
genomic: TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAGgtgagtagta ... tgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
EST: gi|33674839|gb|CF303078.1|CF303078
EST:     TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAG                         ACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
genomic: TAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAGgtgagtagta ... tgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
EST: gi|58661919|gb|CK050599.1|CK050599
EST:     CTTCACTCCAAAAG                         ACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC
genomic: CTTCACTCCAAAAGgtgagtagta ... tgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 70 (upstream exon), 67 (downstream exon)
Score: 37

ACGTTTTCTCATACCCAGAAGATGATAGCGTTGAAGATCCACTATTAGCGCAGCATTTGTCACATTTTGGTATTGATTTTTCTTCACTCCAAAAGgtgagtagta ... tgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGACTGGAATAGAATACAAGAAAGTGGCAAAGATGCCGAACTTTTATACGGCC




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gcttgtctatctgtctagtttagaggctatttgatgtgacccttttgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGACTGGAATAGAATACAAGAAAGTGGCAAAGATGCCGAACTTTTATACGGCC
                            atttgat  putative branch site (score: 37)
                                       cccttttgtt  putative PPT
                           tatttgat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tattctgtagcatctcatgttcatactgtgttttcccctttatatgcttgtctatctgtctagtttagaggctatttgatgtgacccttttgttgtgcagACCGAGATGACTACTGCTGAAAGAGAGCTTGATCACAACACAAATTATGACTGGAATAGAATACAAGAAAGTGGCAAAGATGCCGAACTTTTATACGGCC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGATG
- - ctgtagc
- - - - - - - -catgttc
- - - - - - - - - - - - - tgtgttt
- - - - - - - - - - - - - - - - - - - - - -atgcttg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttgatgt