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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagattgccattgcctcttgtactcttctgaattctgaagagttatcatgttttgctgaccatttcttgtcaaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATCCAGATCAACGGTGGCACTATCGCCGACAAGGTGGACTATGGCTACAAGT

Basic information

species Oryza sativa
transcript LOC_Os12g07010.1
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os12g07010.1 (Oryza sativa), 3'ss of exon 4
lower sequence: AT1G43170.7 (Arabidopsis thaliana), 3'ss of exon 4
gtgagattgcc-attgcctct---tgtactcttctgaattctgaagagttatcatgttttgctgaccatttcttgtca-----aaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATCCAGATCAACGGTGGCACTATCGCCGACAAGGTGGACTATGGCTACAAGT
|| || ||||  ||||  |     |||| | |  | || |||   |   || | | | || ||||| |||   ||| |     || | |||||||| ||||||||||| ||||||||||||||||||||| | ||||||||||||||||| ||||| || || ||| | ||||| |||| || |||||  |
gtaagtttgcttattgaatggaagtgta-tatcataaactcttctgcacta-cgt-tcttactgacaattctatgtgacctctaattacagATCAGGAAGATGAAGGGATTGAAGCAGAAGAAGGCTCACATGATGGAGATCCAGATCAATGGTGGTACCATTGCCCAGAAGGTTGACTTTGCCTACAGTT

upper sequence: LOC_Os12g07010.1 (Oryza sativa), 3'ss of exon 4
lower sequence: Vv18s0072g00340.t01 (Vitis vinifera), 3'ss of exon 4
----gtga-gattgccattgcctc----------ttgtactcttctgaattctgaagagttatcatgttttgctgaccatttcttgtcaaaattc---agATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATCCAGATCAACGGTGGCACTATCGCCGACAAGGTGGACTATGGCTACAAGT
     ||| | ||| || | | ||          |    ||||  |     || ||   |  | ||  | |    |   | ||| ||||   |     |||| || ||||||||||| |||||||||||||| || ||| | |||||||||||| |||| ||||| || || ||  | || ||||| ||||  ||    |
ttatttgacgcttgtcaatacttcaaaatttagttccagctctcatatcccctaaatgatccttatcctctaactaatttctctggtcatggtgttatagATTAGGAAGATGAAGGGATTGAAGCAGAAGAAAGCACACTTGATGGAGATCCAGGTCAATGGTGGAACCATTGCTCAGAAAGTGGATTATGCTTATGGTT

upper sequence: LOC_Os12g07010.1 (Oryza sativa), 3'ss of exon 4
lower sequence: Vv12s0035g01020.t01 (Vitis vinifera), 3'ss of exon 3
---------------------gtgagatt--gccattgcctcttgtactcttctgaattctgaagagttatcatgttttgctgac-catttcttgtcaaa---attc--agATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATCCAGATCAACGGTGGCACTATCGCCGACAAGGTGGACTATGGCTACAAGT
                      || ||||     | |     ||  ||  || | ||| |     |   || || |    |||||  ||    | |  ||   |||   |||| ||||| ||||||||| |||||||||| ||||||||||| |||||||| ||| | || |||||   | | ||  | ||||| ||||||| |||    |
gtagttttccatcaccaatatttgggattcaaagactttaggtttcacatttataaatgcaagttaccaattatttggcactgacatatcctatatggaatggatttgtagATTAGAAAAATGAAGGGCCTGAAGCAGAAAAAGGCTCACCTTATGGAGATTCAGGTGAATGGTGGAGATGTTGCTAAGAAGGTTGACTATGCCTATGGTT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|3107503|gb|D43243.1|D43243
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGANAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTNATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|66914330|gb|CX101178.1|CX101178
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|700083|gb|D46374.1|D46374
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|58606449|gb|CK034482.1|CK034482
EST:     AGAAGATGAAGAAGTGCGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|58681074|gb|CK069761.1|CK069761
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|58652963|gb|CK041643.1|CK041643
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATG
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATG
EST: gi|29643121|gb|CB648128.1|CB648128
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|2428123|gb|C72586.1|C72586
EST:     GAAGATGAAGAAGTACGNATCTATTGTTCGTGTTATTTGCCCACACTCAG                         ATCAGAAAGATGAAGGGNTTTAAGCAGAGGA-GGCTCACCTCATGG
genomic: -AAGATGAAGAAGTACGCATCTATTGTTCGTGTTATT-GCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGG
EST: gi|29660813|gb|CB657088.1|CB657088
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|29680798|gb|CB677073.1|CB677073
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|33682543|gb|CF310782.1|CF310782
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAGAGATG
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATG
EST: gi|218495500|gb|FG951241.1|FG951241
EST:     CGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: CGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|218493886|gb|FG946859.1|FG946859
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|58663821|gb|CK052507.1|CK052507
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|58611003|gb|CK038715.1|CK038715
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAG
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAG
EST: gi|33793525|gb|CF322646.1|CF322646
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|14192019|gb|AU184230.1|AU184230
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|33685065|gb|CF313304.1|CF313304
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|33655921|gb|CF278535.1|CF278535
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGC
EST: gi|58603191|gb|CK013719.1|CK013719
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCCGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|218501376|gb|FG950516.1|FG950516
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCA
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCA
EST: gi|3107792|gb|D43532.1|D43532
EST:     CCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: CCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|33808500|gb|CF330137.1|CF330137
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCATAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|58683823|gb|CK072510.1|CK072510
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGGTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|287230|gb|D16034.1|D16034
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|33810802|gb|CF331292.1|CF331292
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAG
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAG
EST: gi|58604432|gb|CK014960.1|CK014960
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|58585664|gb|CF983972.1|CF983972
EST:     GAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAGGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: GAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|218480893|gb|FG950408.1|FG950408
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|117251520|gb|CT844200.1|CT844200
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|218485951|gb|FG959525.1|FG959525
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAA-AAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|58682184|gb|CK070871.1|CK070871
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|33806231|gb|CF328996.1|CF328996
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
EST: gi|700801|gb|D47092.1|D47092
EST:     AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAG                         ATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC
genomic: AGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 75 (upstream exon), 73 (downstream exon)
Score: 217

CCTTAAGTATGACAGTGATGCTGGCAAGAAAGAAATCCAGATGCAACTTGAGAAGATGAAGAAGTACGCATCTATTGTTCGTGTTATTGCCCACACTCAGgtgagattgc ... caaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATCCAGATCAACGGTGGCACTATCGCCGACAAGGTGGACTATGGCTACAAGT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttctgaattctgaagagttatcatgttttgctgaccatttcttgtcaaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATCCAGATCAACGGTGGCACTATCGCCGACAAGGTGGACTATGGCTACAAGT
                            tgctgac  putative branch site (score: 217)
                                     tttcttgtc  putative PPT
                                            tcaaaatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagattgccattgcctcttgtactcttctgaattctgaagagttatcatgttttgctgaccatttcttgtcaaaattcagATCAGAAAGATGAAGGGCTTGAAGCAGAAGAAGGCTCACCTCATGGAGATCCAGATCAACGGTGGCACTATCGCCGACAAGGTGGACTATGGCTACAAGT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGATCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGTGGA