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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...atgtttggtatatgtatatgccaattatttaatttcatttaatcttataagaaattacatgggtttgtatatgctgttaatatgtatatttgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAG

Basic information

species Oryza sativa
transcript LOC_Os11g28300.1
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os11g28300.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GRMZM2G151863_T01 (Zea mays), 3'ss of exon 4
atgtttggtatatgtatatgccaa-ttatttaatttcat---ttaatct--tataagaaattacatgggtttgtatatgctgttaatatgtatatttgttctc--------aagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAG
              | |||||||| || |||  ||| ||    ||| ||  |   | || ||   |  | || | ||||||| | | || | |       |||        |||||| | |||||||||||||| || ||||| ||||||||||||||||||||||||||||| |||||||||||||
--------------tctatgccaaattctttgtttttatagtctaagctgttgccacaatttttttttgattctctatgctgcttacatatttgcccaccctctccccaacaagGTATTGTCAACTGGACTTGGCAGAATAGTGCCCAACTTGATATCAAGGAAGCACACTAATAGTGCTGCTACAG

upper sequence: LOC_Os11g28300.1 (Oryza sativa), 3'ss of exon 4
lower sequence: AT5G36290.1 (Arabidopsis thaliana), 3'ss of exon 5
atgtttggtatatgtatatgccaattatttaatttcatttaatcttataagaaattacatgggtttgtatatgctgttaatatgtatatttgttc--tcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAG
 |   | | | || ||  ||    |   || |||      ||  | ||   |    | | ||  |||| ||  |||||   || | | |   ||   |  || |||| || |||||||||||||||||||| || ||||||||||| ||||||||||| || |||||||||||||
gtaagttgaaaat-tacttgtatgtatattcattgtgggaaaaatcatcgtactacagagggtattgtcta-actgttgtgatttttctgaattgagtttagATACTTTCTACTGGACTTGGTAGGATAGTGCCAAACTTGATATCGAGGAAGCACACCAATAGTGCTGCTACAG

upper sequence: LOC_Os11g28300.1 (Oryza sativa), 3'ss of exon 4
lower sequence: Vv04s0023g00380.t01 (Vitis vinifera), 3'ss of exon 4
atgtttggtatatgtatatgccaattatttaatttcatttaatcttataagaaattacatgggtttgtatatgct---gttaatatgtatatttgttctca--agGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAG
  ||||  | ||  |  |    || |||  |     ||||| | ||  |||   |  | |    |    | ||     || ||  |   | ||  | |  |  ||||||| || ||||| ||||| ||||| || || || ||||||||||||||||| || || ||||| |||||||
-ggttttctgtagtttcaga--aactatgaagcacgatttattatt--aagttttcccttattctcagttttgtcaaggtcaaagttgttgttcatgccaattagGTACTCTCCACTGGGCTTGGCAGGATTGTGCCAAATTTGATATCAAGGAAGCATACAAATAGTGCAGCTACAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|33804188|gb|CF327969.1|CF327969
EST:     CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACG                         GTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
genomic: CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGgtgagctcct ... tgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
EST: gi|88390701|gb|CI609326.1|CI609326
EST:     CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGAGCTCTAAGTGATGACG                         GTACTATCAACTGGA
genomic: CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGgtgagctcct ... tgttctcaagGTACTATCAACTGGA
EST: gi|44678067|gb|CR291501.1|CR291501
EST:     CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACG                         GTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
genomic: CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGgtgagctcct ... tgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
EST: gi|218479784|gb|FG960708.1|FG960708
EST:     CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACG                         GTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
genomic: CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGgtgagctcct ... tgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
EST: gi|33677157|gb|CF305396.1|CF305396
EST:     CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACG                         GTACTATCAACTGGAC
genomic: CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGgtgagctcct ... tgttctcaagGTACTATCAACTGGAC
EST: gi|66912648|gb|CX099496.1|CX099496
EST:     CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACG                         GTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
genomic: CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGgtgagctcct ... tgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
EST: gi|58589289|gb|CF987597.1|CF987597
EST:     CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACG                         GTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
genomic: CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGgtgagctcct ... tgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
EST: gi|42592205|gb|CK738691.1|CK738691
EST:     CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACG                         GTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
genomic: CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGgtgagctcct ... tgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
EST: gi|33687017|gb|CF315256.1|CF315256
EST:     CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACG                         GTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG
genomic: CCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGgtgagctcct ... tgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 74 (upstream exon), 56 (downstream exon)
Score: 18

ATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGgtgagctcct ... tgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tataagaaattacatgggtttgtatatgctgttaatatgtatatttgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAG
                             tgttaat  putative branch site (score: 18)
                                           tttgttctc  putative PPT
                               ttaatatgtatatttg  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
atgtttggtatatgtatatgccaattatttaatttcatttaatcttataagaaattacatgggtttgtatatgctgttaatatgtatatttgttctcaagGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGCT
atgtttg
- - - - - tatgtat
- - - - - - - - - - - - - - - - tttcatt
- - - - - - - - - - - - - - - - - - - - - - - ataagaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - ttacatg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgctgtt