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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ctttgttaggcatggattctcttagattatttgacttccttccttttaaagactaggcatgacacgtttctctacgccattaacatattcacctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT

Basic information

species Oryza sativa
transcript LOC_Os08g15040.1
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os08g15040.2 (Oryza sativa), 3'ss of exon 4
lower sequence: GRMZM2G147726_T01 (Zea mays), 3'ss of exon 6
-ctttgttaggcatggattctcttagattatttgacttccttccttttaaagactag-gcatgacacgtttctcta-cgccattaacatattcacctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT
    | | ||   |  |  |  |    ||   |||  | | |  ||| |   ||||   |||   |  || |  ||    ||||  ||| |   | |||  |||||||||||| | |||||||||||||| |||||||||||||| |||||||| |||||||| |||||||||||||||||||| ||||||||||| || |||
tgaatatcagaactataaccattacttttgcatga--tgcattttttcatgaactaatacatttaattttccattaatatcattgtcatgtgtgcatttc-agGCCATACTGGCGGTGCTACTTTCCAAACACCCAGGCTATCATATATGTTGTTGATTCAAGCGATACAGATAGGCTTGTAACAGCAAAAGAAGAATTCCAT

upper sequence: LOC_Os08g15040.2 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA17G07390.1 (Glycine max), 3'ss of exon 4
ctttgttaggcatggattctcttagattatttgacttcct-tccttttaaagactaggcatgacacgtttctctacgccattaacatattcacctttt-----tagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT
            || |||  |||    ||| | |     | |   | ||| | | |   ||  | |     | |      ||    | || || ||||     ||||||||| ||||| || ||||||||||||||||| || || || |||||||| |||||||||||    ||||||||||| |  || || || ||||| |||
------acactttgtattgcctttatatatgttatacattatagatgtaatgtccatttatttctcccacttttttttggtttgattttttactttttgtccatagGCCATATTGGAGGTGTTACTTTCCAAATACCCAAGCAATAATCTATGTTGTTGATTCAAGTGACGTTGATAGGCTTGTGATAGCTAAGGAGGAGTTCCAT

upper sequence: LOC_Os08g15040.2 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA13G01270.1 (Glycine max), 3'ss of exon 4
--ctttgt-taggcatggattctcttagattatttgacttccttccttttaaagactaggcatgacacgtttctctacgccattaacatattcacctttt-----tagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT
  |||||| |   |||    | |  ||||||||  ||  |  |  |      |   ||    |  ||| ||| | |     |||    | || || ||||     ||||||||| ||||| || |||||||||||||| || || || || |||||||| |||||||||||    ||||||||||| |  || || || ||||| |||
cactttgtattaccattatgtgttatagattatc-gatgtaatgtc------aatttatttctcccacttttttttgtttgattgtt-tttttactttttgtccatagGCCATATTGGAGGTGTTACTTTCCAAATACTCAAGCAATAATCTATGTTGTTGATTCAAGTGACGTTGATAGGCTTGTGATAGCTAAGGAGGAGTTCCAT

upper sequence: LOC_Os08g15040.2 (Oryza sativa), 3'ss of exon 4
lower sequence: Vv11s0052g01720.t01 (Vitis vinifera), 3'ss of exon 5
--ctttgttaggcatggattctcttag--attatttgacttccttccttttaaagactaggcatgacacgtttctctacgccattaacatattcacctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT
   | | | |        |||| || |  || |||||  |   ||    | || | |   | || |   | | || |      || |  | ||         |||||||| ||||||||||| ||||||||||| || || || || |||||||| |||||||||||||| |||||  | ||||  || ||||| |||||||||
caatgtttcaatttacctttcttttgggcataatttg--taagtttgcatcaagggcacaggatcatcagctgct-tgtttggtttatgt-tttttgggcacagGCCATATTGGAGATGCTATTTTCCAAATACTCAAGCCATAATATATGTTGTTGATTCAAGTGATACTGATAGATTGGTAATAGCTAAAGATGAGTTTCAT

upper sequence: LOC_Os08g15040.2 (Oryza sativa), 3'ss of exon 4
lower sequence: Vv04s0008g07360.t01 (Vitis vinifera), 3'ss of exon 4
ctttgttaggcatggattctcttagattatttgacttccttccttttaaagactaggcatgacacgtttctctacgccattaacatattcaccttttta--gGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT
   ||||   |   ||  ||  |||||||||   ||   | | ||      | ||    ||  |    |     |  ||      | ||||  || |||  |||||||||||||||| || || |||||||| || || || ||||||||||| || |||||||| || |||||  ||||||  || || || || ||||||
--ctgttgttctgtgaagcttatagattattatgctgattgctttaaggttattatctctgcaattcattggctcatcaactgtttcttcattttgttacagGCCATACTGGAGATGTTATTTCCCAAATACTCAAGCAATAATTTATGTTGTTGACTCAAGTGACACTGATAGAATTGTAATAGCCAAGGATGAATTTCAT

upper sequence: LOC_Os08g15040.2 (Oryza sativa), 3'ss of exon 4
lower sequence: EFJ37645 (Selaginella moellendorffii), 3'ss of exon 4
ctttgttaggcatggattctcttagattatttgacttccttccttttaaagactaggcatgacacgtttctctacgccattaacatattcacctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT
            ||  |  ||   | | ||||   ||| ||  ||| ||| |||  |        ||||    ||           | ||| | ||  || || ||||||||||||||||||||||| || |||||| | ||||| ||||| ||||| | ||| || || ||  | | ||| ||||| || ||  | || 
-----------gtgagtgatcgcgg-tcattt--tttcttttgtttcaaacactttg--------gtttgctgac-----------actcatcattc-agACCTTACTGGAGATGCTACTTTCCAAACACACAGGCTGTGATTTACGTTGTTGATTCGACTGACACGGAAAGAATGGCAACTGCAAAGGACGAACTCCAC

upper sequence: LOC_Os08g15040.2 (Oryza sativa), 3'ss of exon 4
lower sequence: EFJ14920 (Selaginella moellendorffii), 3'ss of exon 4
ctttgttaggcatggattctcttagattatttgacttccttccttttaaagactaggcatgacacgtttctctacgccattaacatattcacctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT
                               ||| |  |||   ||| ||           |||| ||  | | |     | |||   ||| | ||  || || ||||||||||||||||||||||| || |||||| | ||||| ||||| ||||| | ||| || || ||  | | ||| ||||| || ||  | || 
------------------------------gtgagtatcttttgtttcaa-----------acactttggtttgc-----tgaca--ctcatcattc-agACCTTACTGGAGATGCTACTTTCCAAACACACAGGCTGTGATTTACGTTGTTGATTCGACTGACACGGAAAGAATGGCAACTGCAAAGGACGAACTCCAC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|86478438|gb|CI121071.1|CI121071
EST:     ATCTCGGTGGTCAAACAAGCATCAG                         GCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
genomic: ATCTCGGTGGTCAAACAAGCATCAGgtaccatctc ... acctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
EST: gi|8956150|gb|BE229954.1|BE229954
EST:     ATCTCGGTGGTCAAACAAGCATCAG                         GCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
genomic: ATCTCGGTGGTCAAACAAGCATCAGgtaccatctc ... acctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
EST: gi|27554607|gb|CA998507.1|CA998507
EST:     ATCTCGGTGGTCAAACAAGCATCAA                         GCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
genomic: ATCTCGGTGGTCAAACAAGCATCAGgtaccatctc ... acctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
EST: gi|88383993|gb|CI631883.1|CI631883
EST:     ATCTCGGTGGTCAAACAAGCATCAG                         GCCATACTGGAGATGCTACTTTCCAACATACC-AGGCTATCATTTATGTTG
genomic: ATCTCGGTGGTCAAACAAGCATCAGgtaccatctc ... acctttttagGCCATACTGGAGATGCTACTTTCCAA-ATACCCAGGCTATCATTTATGTTG
EST: gi|218486133|gb|FG956315.1|FG956315
EST:     ATCTCGGTGGTCAAACAAGCATCAG                         GCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
genomic: ATCTCGGTGGTCAAACAAGCATCAGgtaccatctc ... acctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
EST: gi|87011925|gb|CI283812.1|CI283812
EST:     AAACAAGCATCAG                         GCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
genomic: AAACAAGCATCAGgtaccatctc ... acctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
EST: gi|27554323|gb|CA998223.1|CA998223
EST:     ATCTCGGTGGTCAAACAAGCATCAG                         GCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT
genomic: ATCTCGGTGGTCAAACAAGCATCAGgtaccatctc ... acctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 19 (upstream exon), 70 (downstream exon)
Score: 33

GTGGTCAAACAAGCATCAGgtaccatctc ... acctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttaaagactaggcatgacacgtttctctacgccattaacatattcacctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT
                                cattaac  putative branch site (score: 33)
                                          ttcaccttttt  putative PPT
                                 attaacatatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ctttgttaggcatggattctcttagattatttgacttccttccttttaaagactaggcatgacacgtttctctacgccattaacatattcacctttttagGCCATACTGGAGATGCTACTTTCCAAATACCCAGGCTATCATTTATGTTGTCGATTCAAGTGATACAGATAGGCTTGTAACGGCAAAAGAAGAGTTTCAT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG
- - - - -gcatgga
- - - - - - - - - - - - - - - - - - -ccttcct
- - - - - - - - - - - - - - - - - - - - - - - - - - - -ggcatga