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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ctttaaaggggcttctccttcagtttttccctcctaggaaatagctgtccaaaacaaccgattaatttttctaatgagcaaatttctttgtgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGTCCCTGTTGGAATGTCCATCTTAGAAGCTGCTCATGAAAATGACATCGAA

Basic information

species Oryza sativa
transcript LOC_Os07g01930.1
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os07g01930.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GRMZM2G013607_T02 (Zea mays), 3'ss of exon 4
ctttaaaggggcttctccttcagtttttccctcctaggaaatagct--gtccaaaacaaccgattaatttttctaatgagcaaatttctttgtgctgc--acagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGTCCCTGTTGGAATGTCCATCTTAGAAGCTGCTCATGAAAATGACATCGAA
    |      ||      | ||| ||   ||   |  || ||  |  |    || ||  |   | ||  || |   |   |  |||||   | |||   |||| ||||| ||||||||||| |||||||||||| ||||  |||||||  |||| || |||||||||||||| |||||||||||||||||||| ||||| || 
----agttttactgaattgttagtgttgagctttcaataattatttagggtagaagcagtcctctgatgattgttgaggttatttttctccatactgtgaacagAATCTCGGTAACATTTGTCAACAAGGATGGCTCAGAAAAGACAATATGTGTTCCAGTTGGAATGTCCATGTTAGAAGCTGCTCATGAAAACGACATAGAG

upper sequence: LOC_Os07g01930.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA08G07780.1 (Glycine max), 3'ss of exon 1
ctttaaaggggcttctccttcagtttttccctcctaggaaatagctgtccaaaacaaccgattaatttttctaatgagcaaatttctttgtg------ctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGTCCCTGTTGGAATGTCCATCTTAGAAGCTGCTCATGAAAATGACATCGAA
           || ||  |  |  ||     |   | |  |      |  |||| ||  |   | || |  |  ||  |     | |||| |       |   |||||| |||||||| ||| |  ||||||||||    ||| ||   ||||  ||||||||||||||||| || |||||||| ||||||||||| ||||| |||
------cacaactactattgtaccttgcttatatcatgtgagtttattaaaaaaaaaatgcacagttgtcattctgctccttactttttgaggcaacattatgcagGATATCTGTAACTTTTATTGACAAGGATGGTGAGGAGAAGCATATCAAAGTCCCTGTTGGAATGTCTATGTTAGAAGCAGCTCATGAAAACGACATTGAA

upper sequence: LOC_Os07g01930.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA13G17370.2 (Glycine max), 3'ss of exon 3
-ctttaaaggggcttctccttcagtttttccctcctaggaaatagctgtccaaaacaaccgattaatttttctaatgagcaaatttctttgtgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGTCCCTGTTGGAATGTCCATCTTAGAAGCTGCTCATGAAAATGACATCGAA
      || |  |  |  ||   ||  ||  ||| ||     ||   |     || |    || | ||   ||  | |     |          |   |||||| |||||||| ||| |  | ||||||||    ||| ||   ||||  ||||||||||||||||| || |||||||||||||||||||||||||| |||
agacagaatgcacaacgactgttgtactttgctcgtatattttactagatggcaatagtgcatca-ttccgctccttactttttgaggcaacattatgcagGATATCTGTAACTTTTATTGATAAGGATGGTGAGGAGAAGCATATCAAAGTCCCTGTTGGAATGTCTATGTTAGAAGCTGCTCATGAAAATGACATTGAA

upper sequence: LOC_Os07g01930.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA17G05130.1 (Glycine max), 3'ss of exon 3
ctttaaaggggcttctccttcagtttttccctcctaggaaatagctgtccaaaacaaccgat-taatttttctaatgagcaaatttctttgtgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGTCCCTGTTGGAATGTCCATCTTAGAAGCTGCTCATGAAAATGACATCGAA
  |  | |    |  | | ||| | |     |  ||  ||| |        | |  || | | | ||| | ||  | |     |          |   |||||| |||||||| ||| |  | ||||| ||    ||| ||   ||||  ||||||||||||||||| || |||||||||||||||||||| ||||| |||
actagatgtcaatagtgcatca-tgtgagtttattaaaaaaaaaaaaaaagagagcacagttgtcattctgctccttactttttgaggcaacattatgcagGATATCTGTAACTTTTATTGATAAGGACGGTGAGGAGAAGCATATCAAAGTCCCTGTTGGAATGTCTATGTTAGAAGCTGCTCATGAAAACGACATAGAA

upper sequence: LOC_Os07g01930.1 (Oryza sativa), 3'ss of exon 4
lower sequence: Vv11s0016g00530.t01 (Vitis vinifera), 3'ss of exon 4
---ctttaaaggggcttctccttcagtttttccctcctaggaaatagctg--tccaaaacaaccgattaatttttctaatgagcaaatttctttgtgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGTCCCTGTTGGAATGTCCATCTTAGAAGCTGCTCATGAAAATGACATCGAA
     |||      | ||    |      || |||| ||    ||  |||  |  | |  | ||   |  || |||| ||    | ||||  ||||||     ||||| |||||  |||||||  | ||||||||    || ||    || || |||||  |||||||||| || ||||||||||||||||||||||| || |||
caatcttagttacacatcatggttctacatttcctcttaactcatttctgcatgtagattaccctggtgtttcttctcattcatatatttaattgtgc-----agGATATCTGTGTCATTTGTTGATAAGGATGGAGAGGAACATCACATTAGGGTCCCCATTGGAATGTCTATGTTAGAAGCTGCTCATGAAAATGATATAGAA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|10794608|gb|AU108574.1|AU108574
EST:     ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAA                         GATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
genomic: ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAAgtaagtatat ... tgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
EST: gi|218490073|gb|FG948574.1|FG948574
EST:     ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCA-GGAGAA                         GATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
genomic: ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAAgtaagtatat ... tgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
EST: gi|88461626|gb|CI695464.1|CI695464
EST:     ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAA                         GATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
genomic: ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAAgtaagtatat ... tgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
EST: gi|66917010|gb|CX103858.1|CX103858
EST:     ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAA                         GATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
genomic: ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAAgtaagtatat ... tgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
EST: gi|58601999|gb|CK012527.1|CK012527
EST:     ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAA                         GATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
genomic: ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAAgtaagtatat ... tgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
EST: gi|24468825|gb|CA305774.1|CA305774
EST:     ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAA                         GATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
genomic: ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAAgtaagtatat ... tgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
EST: gi|33680135|gb|CF308374.1|CF308374
EST:     ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAA                         GATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
genomic: ACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAAgtaagtatat ... tgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
EST: gi|11443010|gb|BF430897.1|BF430897
EST:     GGCAGTCAGAGCAAGGAGAA                         GATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT
genomic: GGCAGTCAGAGCAAGGAGAAgtaagtatat ... tgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 68 (upstream exon), 66 (downstream exon)
Score: 9

AAGAACATCTTATTCTCCACAGCAACAACTAGTAGTGATCGTGACGATGGCAGTCAGAGCAAGGAGAAgtaagtatat ... tgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGTCCCTGTTGGAATGTCCATCTTAGAAGCTGCTCATGAAAATGACATCGAA




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgtccaaaacaaccgattaatttttctaatgagcaaatttctttgtgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGTCCCTGTTGGAATGTCCATCTTAGAAGCTGCTCATGAAAATGACATCGAA
                       ttctaat  putative branch site (score: 9)
                                     tttctttgt  putative PPT
               attaatttttctaatg  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ctttaaaggggcttctccttcagtttttccctcctaggaaatagctgtccaaaacaaccgattaatttttctaatgagcaaatttctttgtgctgcacagGATCTCTGTAACATTTGTGAACAAGGATGGCACAGAGCAGACAATCAGTGTCCCTGTTGGAATGTCCATCTTAGAAGCTGCTCATGAAAATGACATCGAA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGCT
- - - - -ggcttct
- - - - - - - - ccttcag
- - - - - - - - - - - - - - - - - -aggaaata
- - - - - - - - - - - - - - - - - - - - - - - - - aaaacaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gagcaaa