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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtatagtacctgtgcatgctaatttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG

Basic information

species Oryza sativa
transcript LOC_Os06g05080.1
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os06g05080.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA13G43980.1 (Glycine max), 3'ss of exon 3
gtatagtacctgtgcatgctaat-ttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatatttt-cagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG
                 |  | | |||||||| |||      | | ||| ||    |  || |||| | | ||  ||||     || ||            ||  ||||  | | || | ||| | ||     || ||       | || | |||| |  ||||||||||| || ||||||||| ||||||||||||||||    |||||||| || ||| | ||||||||   |
-----------------gtaagtgttccttgtctttctttatttatgaatct----gtgtgcaatccttttcttaatctg----atgaa------------aaatacca--gaagttga-ttatattgtgtggtgaaaaccccctttttctgcagAAGGTGAGCATGATGTTGTCTGGTTTTGGCTGGAGAAAGGCAAGCCTATAGAATGCCCAGTGTGCTCACAGTATTTTAAG

upper sequence: LOC_Os06g05080.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA07G03680.2 (Glycine max), 3'ss of exon 4
gtatagtacctgtgcatgctaatttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG
   | ||   | | ||   ||  |||||||||| |      ||| |   ||||| | |||  | ||||| |   |  |     || || |    | || |  |     ||||  || | | |    |  ||   |     |||||   | ||||||| ||||||||||| || ||||||||| ||||||||| |||||| |||||||| || || ||| | ||||||||    
--gtggtgagtat-cagaata--ttccttgtttct----cttaatg--tcttcttgcatgtgaacttcttcccaaggtg----atgaaaaaagctagaatt-ctggttatggtgttta-tgagggggtgaacaacttgttttgat---tacagAGGATGAGCATGATGTTGTCTGGTTTTGGCTGGAGAAAGACAAGCCCCATGAATGTCCAGTGTGCGCACAGTATTTTGTT

upper sequence: LOC_Os06g05080.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA07G03680.3 (Glycine max), 3'ss of exon 4
gtatagtacctgtgcatgctaat-ttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG
             | ||   ||| |||||||||| |      ||| |   ||||| | |||  | ||||| |   |  |     || || |    | || |  |     ||||  || | | |    |  ||   |     |||||   | ||||||| ||||||||||| || ||||||||| ||||||||| |||||| |||||||| || || ||| | ||||||||    
-------------gtatcagaatattccttgtttct----cttaatg--tcttcttgcatgtgaacttcttcccaaggtg----atgaaaaaagctagaatt-ctggttatggtgttta-tgagggggtgaacaacttgttttgat---tacagagGATGAGCATGATGTTGTCTGGTTTTGGCTGGAGAAAGACAAGCCCCATGAATGTCCAGTGTGCGCACAGTATTTTGTT

upper sequence: LOC_Os06g05080.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA15G01360.1 (Glycine max), 3'ss of exon 4
gtatagtacctgtgcatgctaat-ttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgca-aattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG
                 |  | | |||||||| |||      | | ||| ||    |  || || | | | ||  ||||     || ||            || |||||  | | || | ||| | ||     ||  ||       | | | |||| || ||||||||||| || ||||||||| ||||||||||||||||  | ||||| || || ||| | ||||||||   |
-----------------gtaagtgttccttgtcttt----ctttacgaatct----gtgtgcaagccttttcttaatctg----atgaa------------aaacacca--gaagttga-ttatattgtgtggttaaaaccccctttttctgcagAAGATGAGCATGATGTTGTCTGGTTTTGGCTGGAGAAAGGCAAGCCTAAAGAATGTCCAGTGTGCTCACAGTATTTTAAG

upper sequence: LOC_Os06g05080.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA07G03680.1 (Glycine max), 3'ss of exon 4
gtatagtacctgtgcatgctaat-ttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG
          || | ||   ||| |||||||||| |      ||| |   ||||| | |||  | ||||| |   |  |     || || |    | || |  |     ||||  || | | |    |  ||   |     |||||   | ||||||| ||||||||||| || ||||||||| ||||||||| |||||| |||||||| || || ||| | ||||||||    
---------gtgagtatcagaatattccttgtttct----cttaatg--tcttcttgcatgtgaacttcttcccaaggtg----atgaaaaaagctagaatt-ctggttatggtgttta-tgagggggtgaacaacttgttttgat---tacagAGGATGAGCATGATGTTGTCTGGTTTTGGCTGGAGAAAGACAAGCCCCATGAATGTCCAGTGTGCGCACAGTATTTTGTT

upper sequence: LOC_Os06g05080.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA08G22410.1 (Glycine max), 3'ss of exon 4
gtatagtacctgtgcatgctaatttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG
           |||  | | |   |  |  || ||   || ||| |   ||||| | |||  | ||||| |      |     || || |    | || |        ||||  || | | |    |  ||   |     |||||   ||||||||| ||||||||||| || ||||||||| ||||||||| |||||| || ||||| || || ||| | ||||||||    
-----------gtgagtaccagaatattccttgtttctct-taatg--tcttcttgcatgtgaccttcttcccagggtg----atgaaaaaggctagaattcttggttatggtgttta-tgagggggtgaacgacttgttttgat---ttcagAGGATGAGCATGATGTTGTCTGGTTTTGGCTGGAGAAAGACAAGCCCCACGAATGTCCAGTGTGCGCACAGTATTTTGTT

upper sequence: LOC_Os06g05080.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA07G03680.4 (Glycine max), 3'ss of exon 4
gtatagtacctgtgcatgctaat-ttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTC-TCG-------------------
          || | ||   ||| |||||||||| |      ||| |   ||||| | |||  | ||||| |   |  |     || || |    | || |  |     ||||  || | | |    |  ||   |     |||||   | ||||||| ||||||||||| || ||||||||| ||||||||| |||||| |||||||| || || ||| | ||||||||  | |                   
---------gtgagtatcagaatattccttgtttct----cttaatg--tcttcttgcatgtgaacttcttcccaaggtg----atgaaaaaagctagaatt-ctggttatggtgttta-tgagggggtgaacaacttgttttgat---tacagAGGATGAGCATGATGTTGTCTGGTTTTGGCTGGAGAAAGACAAGCCCCATGAATGTCCAGTGTGCGCACAGTATTTTGTTGTAAGTTCATAATGACTTTT

upper sequence: LOC_Os06g05080.1 (Oryza sativa), 3'ss of exon 4
lower sequence: AT1G80230.1 (Arabidopsis thaliana), 3'ss of exon 4
gtatagtacctgtgcatgctaatttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatatttt-cagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG
                                                        |  |   | || |  |     |||  | |    || |||    |       | |   | ||    |||    | ||     |  || ||  | | ||||||| ||||||||||| || ||||||||| |||||||||||||| |   |||||||||||| || || ||||| |||  |
--------------------------------------------------------gtgggtcttttttattttgctttcattgtgtgcat-catgatttggtt--tatttttagtagaccaaaggagatatgatggtttttcattctctacagAGGATGAGCATGATGTTGTCTGGTTTTGGCTGGAGAAAGGCAAGTCTTTTGAATGCCCTGTGTGTACACAGTACTTCAAG

upper sequence: LOC_Os06g05080.1 (Oryza sativa), 3'ss of exon 4
lower sequence: AT3G15640.1 (Arabidopsis thaliana), 3'ss of exon 4
gtatagtacctgtgcatgctaatttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG
             | | | |   |||| | | ||  | || ||        |   |||  |  | |  ||    ||| |   | |       ||| || ||   | |||   |  | | |||     | || |        ||| |  | ||||||||||||| ||||| || ||||| ||| |||||||||| ||| |   ||||||||| || ||||||||||| ||   |
-------------gtaagtt--cttccct-tcttcaaactttagtcgcatattatggacaatctttaatcggcaatcgttgttgt-------cattgaata--tatcaagacacattatttaccaatctaacttt--ctcctgaaaactgcagAGGACGAGCACGATGTTGTCTGGTTCTGGCTGGAGAAAGGAAAGTCCTTTGAATGCCCGGTTTGCACTCAGTACTTTGAG

upper sequence: LOC_Os06g05080.1 (Oryza sativa), 3'ss of exon 4
lower sequence: Vv09s0002g06000.t01 (Vitis vinifera), 3'ss of exon 4
gtatagtacctgtgcatgctaatttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattc-atcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG
                 |  |   ||  |   ||   |   | ||  |   | |   ||  | |  | || | ||  |  |  ||    || | ||  | |     | |||     |  ||     |    |||    || ||    | | ||||||| ||||||||||| || ||||||||| |||||||||||||| |||||||||||||||| ||| | ||||||||| ||
-----------------gtaagcctctgtcacttactctctttaattattattttccatttatttatttcttacaattaattaatttgcatcccattaaattctttctaattttggttgctctg-ttatttatttatttgtttttcgtctgcagAGGATGAGCATGATGTTGTCTGGTTTTGGCTGGAGAAAGGCAAGTCACATGAATGCCCTGTGTGCTCACAGTATTTCACG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|86512602|gb|CI155235.1|CI155235
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|38478025|gb|CF962158.1|CF962158
EST:     GTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAGAGGCAAGCCACATG
genomic: GTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|86511030|gb|CI153663.1|CI153663
EST:     CATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: CATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|86599818|gb|CI255980.1|CI255980
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|86432882|gb|CI086493.1|CI086493
EST:     CCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: CCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|27920981|gb|CB096789.1|CB096789
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|86854015|gb|CI252006.1|CI252006
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|14713858|gb|AU197818.1|AU197818
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|88205449|gb|CI205298.1|CI205298
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|88242794|gb|CI240645.1|CI240645
EST:     TGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|25996302|gb|CA767047.1|CA767047
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|86512611|gb|CI155244.1|CI155244
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|87018425|gb|CI252179.1|CI252179
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|38482983|gb|CF966785.1|CF966785
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|86497846|gb|CI140479.1|CI140479
EST:     TCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|58600462|gb|CK010990.1|CK010990
EST:     CAGTNCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: CAGT-CATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|4716340|gb|AU057456.1|AU057456
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
EST: gi|86822729|gb|CI251809.1|CI251809
EST:     TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAG                         AGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG
genomic: TCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 51 (upstream exon), 58 (downstream exon)
Score: 8

GAAGCTCCAGCTGTTATTCAGTCATACTACAACAAAAGGATTGTTGGTTGTCCCGGTGGTGAAGGAGgtatagtacc ... atattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ctaaccaccaatggaattaacttaaactgcaaattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG
                                              tattttc  CT-rich tract
                                     aatgattgatatttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatagtacctgtgcatgctaatttccttgtttttgacctataaagaagcttctaggatgaaatcttctcctgcatcttctctataaatattcatcgactaaccaccaatggaattaacttaaactgcaaattgcaatgattgatattttcagAGGACGAGCATGATGTCGTGTGGTTTTGGTTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGCACTCAGTATTTCTCG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGAA