Skip to another splice site:
1  
 5'  3'   		2  
 5'  3'   		3  
 5'  3'   		4  
 5'  3'   		5  
 5'  3'   		6  
 5'  3'   		7  
 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tatcttgaattctagcttatttcttattttagatccttttatagttgcaagcttagtagttggttctgacttctgaggctcaaactttctgttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA

Basic information

species Oryza sativa
transcript LOC_Os05g25180.1
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os05g25180.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GRMZM2G061980_T01 (Zea mays), 3'ss of exon 4
tatcttgaattctagct-tatttcttattttagatccttttatagttgcaagc--ttagtagttggttctgacttctgaggctcaaactttctgttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA
 |  |||   |  ||    || | | | |||    |||| | |     ||| |  |||||| |      ||       |    |||   ||||   ||  ||||| |||||| ||||||||||||| ||||||||||| || |||||||| |||||||||||| |||||||||| ||||||||||||||||||||||||||||
aacattgttgtacagtaacatgtgtgaattttttcccttctgttagaacaaccaattagtattgcagaatgttacactaactacaa---ttcttcctttttagGGCCAAATGTTAACCCTTGTGGAGTACCTTCTGCTTCTTTATCGTGCTTTGTTGCCAACTTCTGTTTGGTATCGTTTCTTTCTGAACAAGGAATATGGGA

upper sequence: LOC_Os05g25180.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA04G40220.1 (Glycine max), 3'ss of exon 5
tatcttgaatt-ctagcttatttcttattttagatccttttatagttgcaagcttagtagttggttctgacttctga--ggctcaaactttctgttttgct--agGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA
  | || | |  ||  || | || ||     |       |   |  |        ||||  | |||    | |   |    || | | | | |   ||  |  ||||||||||| |||| || || || ||   |||||| || |||||||||||||||||||| || ||||||||||| || ||  |||||||||| ||||| |
-gtttttagtagcttcctcactttttccaacaagcatagtagcatatatttttcaagta--tagtttcctcctacaatcatctgatattatttaacttatttcagGGTCAAATGTTAACTCTGGTCGAGTATACTCTGCTGCTGTATCGTGCCTTGTTGCCAACACCAGTTTGGTACCGATTTTTCTTGAACAAGGATTATGGAA

upper sequence: LOC_Os05g25180.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA18G45010.1 (Glycine max), 3'ss of exon 4
tatcttgaattctagcttatttcttattttagatccttttatagttgcaagcttagtagttggttctgac-ttctgaggctcaaactttctgttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA
| |  |   || |     ||||| |    |      ||    |  |  |   |   |     ||  | || |||||| | |   |   | | |||||  ||||||||||||| ||  | || |||||||| || || || || ||||||||||||||||| || |||||||| |||||||| |||||||| |||||||| |
tctgctatcttttgtaacatttcctggagttatgggttcaggatctatatcatctttctcatgtcattacattctgatggtactatcattt-ttttgtcagGGTCAAATGCTGACTTTAGTTGAATACCTCCTCCTGCTTTACCGTGCCTTGTTGCCAACACCAGTTTGGTATCGTTTCTTCCTGAACAAAGAATATGGAA

upper sequence: LOC_Os05g25180.1 (Oryza sativa), 3'ss of exon 4
lower sequence: GLYMA06G14590.1 (Glycine max), 3'ss of exon 4
--tatcttgaattctagcttatttcttattt-tagatccttttatagttg-caagcttagtagttggttctgacttctgaggctcaaactttctgttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA
  | |  |   |||     | ||||| |     | |      |||| ||  ||||  ||||  ||  | ||    ||    | |   | ||    | || | ||||||||||| ||||  |  | || ||   |||||| || |||||||| ||||||||||| || ||||||||||| || ||  |||||||||| ||||| |
gttttagtagcttcctttctttttctgacagacaaaatagcatatatttttcaagtatagt--ttcctcctacaatcatctgatagtatttaacttatttcaagGGTCAAATGTTAACTTTGATTGAGTATACTCTGCTGCTGTATCGTGCTTTGTTGCCAACACCAGTTTGGTACCGATTTTTCTTGAACAAGGATTATGGAA

upper sequence: LOC_Os05g25180.1 (Oryza sativa), 3'ss of exon 4
lower sequence: AT5G01960.1 (Arabidopsis thaliana), 3'ss of exon 5
--tatcttgaattctagcttatttcttattttagatccttttatagttgcaagcttagtagttggttctgac--ttctgaggctca-aactttctgttttgct--------agGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA
   || | |||   ||   |  |   |    |  | |  | |  |||| ||       | | |   | || |   |||| |   || || ||  |  ||  ||        |||||||| |||||||  | || |||||  |  |||| || |||||  | ||  |||| || ||||| ||||||||||||||| |||||||||| ||||| |
gtgatgtagaaaaatattcttctctgtcccctttagctgtattcagtttcatttggcatggctcactgtggcgtctctgggatacataatttggttattcacttccaatgcagGGTCAATTGCTAACTTTGGTTGAATATTTCATGCTTCTGTATCGCTCTTTACTGCCTACCCCTGTATGGTACCGTTTCTTTTTGAACAAGGATTATGGAA

upper sequence: LOC_Os05g25180.1 (Oryza sativa), 3'ss of exon 4
lower sequence: Vv08s0056g00440.t01 (Vitis vinifera), 3'ss of exon 4
--------------tatcttgaattctagcttattt-cttattttagatccttttatagttgcaagcttagtagttggttctgacttctgaggctcaaactttctgttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA
               ||  ||  ||  ||   ||||  |||  ||||   | |||  | ||  |  |||  |   ||    |     ||||  ||| |    | | |||| | |||||||| |||||||  | || |||||     | ||  | |||||||||||||||||| | ||||| ||||| ||||| ||  ||||||| ||||||||||
gtaaggtaatatgaaattatgtgttaaagtagatttatttaggttagtatcatttgcatttatattctttttgtctgccattacacactga--ctccagacatttatttt-ccagGGTCAACTGCTAACTTTGGTTGAATATACATTACTGTTGTATCGTGCCTTGTTGCCAGCACCTGTCTGGTATCGTTTTTTCTTGAACAAAGAATATGGGA

upper sequence: LOC_Os05g25180.1 (Oryza sativa), 3'ss of exon 4
lower sequence: Vv06s0004g08300.t01 (Vitis vinifera), 3'ss of exon 5
-tatcttgaattctagcttatttcttattttagatcct-tttatagttgcaagcttagtagttggttct----gacttctgaggctcaaactttctgttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA
 || ||| |||  |   ||  | |  |  |||  |  | |||||  ||  |  |||     |   || |     ||||||    | || |  || |||||| | ||||||| |||||||| ||||| || || ||  || | || || |||||||||||||| || || || ||||| || |||||| ||||||| |||||||| |
gtaacttaaataatgaatt--tcccaaagttatttagtgtttatgcttctagtctttcctttatattttccagaacttcttgaccacatatgttttgtttttccagGGTCAGATGCTAACTCTTGTTGAGTATCTGTTGTTGCTGTACCGTGCCTTGTTGCCTACACCAGTCTGGTATCGGTTCTTTTTGAACAAAGAATATGGAA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|88223400|gb|CI223249.1|CI223249
EST:     AACTACCGTAAGCAG                         GGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTG
genomic: AACTACCGTAAGCAGgtgacatttt ... gttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTG
EST: gi|88243065|gb|CI244187.1|CI244187
EST:     ATGTACTATAAGAATAGCAGAGGTCGTAACTACCGTAAGCAG                         GGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTG
genomic: ATGTACTATAAGAATAGCAGAGGTCGTAACTACCGTAAGCAGgtgacatttt ... gttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTG
EST: gi|66920417|gb|CX107265.1|CX107265
EST:     TGCTACTTATGTACTATAAGAATAGCAGAGGTCGTAACTACCGTAAGCAG                         GGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTG
genomic: TGCTACTTATGTACTATAAGAATAGCAGAGGTCGTAACTACCGTAAGCAGgtgacatttt ... gttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTG
EST: gi|86493390|gb|CI136023.1|CI136023
EST:     TGCTACTTATGTACTATAAGAATAGCAGAGGCCGTAACTACCGTAAGCAG                         GGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTG
genomic: TGCTACTTATGTACTATAAGAATAGCAGAGGTCGTAACTACCGTAAGCAGgtgacatttt ... gttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 74 (upstream exon), 74 (downstream exon)
Score: 35

ATACAATGGTTCGCCAGGCAGCAATGGCCATTAAGTGCATGCTACTTATGTACTATAAGAATAGCAGAGGTCGTAACTACCGTAAGCAGgtgacatttt ... gttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA




<











<


<











 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgcaagcttagtagttggttctgacttctgaggctcaaactttctgttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA
                  ttctgac  putative branch site (score: 35)
                                       ctttctgttttgct  putative PPT
                                    aaacttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tatcttgaattctagcttatttcttattttagatccttttatagttgcaagcttagtagttggttctgacttctgaggctcaaactttctgttttgctagGGTCAAATGCTAACCCTTGTGGAATACCTTCTGCTCCTATATCGTGCCTTGTTGCCAACTCCTGTTTGGTACCGTTTCTTTCTGAACAAGGAATATGGGA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAA
- tcttgaa
- - - - - - - - - - - - - - - - - - - - - - -tgcaagc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ggttctg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ctgaggc