ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtttgtgaattgttgctaatttgatgatcagattgatgctgcagttcatttaagttatggtggtattaagcaatattctaccgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACGTATGCACTTTTGGTGCTCTTCTGGGCCGAGATTTATTATCAG

Basic information

species	Oryza sativa
transcript	LOC_Os10g39220.1
intron #	3
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os10g39220.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GRMZM2G174286_T03 (Zea mays), 3'ss of exon 4

gtttgtgaattgttgctaatttgatgatcagattgatgctgca------gttcatttaagtt-----atggtggtattaagcaatattctaccgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACGTATGCACTTTTGGTGCTCTTCTGGGCCGAGATTTATTATCAG
         |||| ||| |||||||||| | | |      |||       ||  |   || |      |  |||| | ||||||| |  || | |||||| | || |||||||||||||||||||| ||||| ||||||||||| || ||||||||| | |||||||||||||| |||||||||||||||
-------gtttgtagctgatttgatgattatactacactcgcataatgaatttgtgggaggtcttgagtaatggtttgaagcaatgtggtaacacagATAATACAGCATGTGCTTCTAGATATGCCTGGGCTTGCATTCTTCACAACATATGCACTTCTAGTGCTCTTCTGGGCAGAGATTTATTATCAG

upper sequence: LOC_Os10g39220.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GRMZM2G003076_T02 (Zea mays), 3'ss of exon 3

gtttgt----gaattgttgctaatttgatgatca---gattgatgctgcagttcatttaagttatggtggtattaagcaatattctaccgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACGTATGCACTTTTGGTGCTCTTCTGGGCCGAGATTTATTATCAG
||||||    || ||| |  | ||   ||  ||    |||  ||      |     || |||  |  |||| | ||||||| |  |||| |||||| | |||||||||||||| |||||||||||||| |||||||||||||| ||||||||||| |||||||||||||| |||||||||||||||
gtttgtaactgatttgattattatactatactcgtgtgatgaatttgttggaggtctttagtactaatggtttgaagcaatgtggtaccacagATAATACAACATGTGCTTCTGGATATGCCCGGGCTTGCATTCTTCACGACATATGCACTTTTAGTGCTCTTCTGGGCTGAGATTTATTATCAG

upper sequence: LOC_Os10g39220.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GLYMA07G28990.1 (Glycine max), 3'ss of exon 3

----------------------------------------gtttgtgaat----tgttgctaatttg--atgatcagattgatgctgcagttcatttaagttatggtggtattaagcaatattctacc-gcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACGTATGCACTTTTGGTGCTCTTCTGGGCCGAGATTTATTATCAG
                                         ||| ||||     | ||  |||||||  ||  ||  |||        | |  | | |  | ||| |     |        || |    | ||||  |||||||  |  | || ||  ||||  | || || ||||| || || |||||||||||||| || |||||||| |||||||||||||||
gtattggagtatagggaatgtggcatttatttttgagttattttttgaacctaatattaataatttgttattttctaattagaatgtgattagactcacttaatgctcactctgcatttgcttgtgtttgtagATTGTTCAACACATCTTGCTTGACGTGCCGAGTCTTGCTTTCTTTACAACATATGCACTTTTGGTCCTGTTCTGGGCTGAGATTTATTATCAG

upper sequence: LOC_Os10g39220.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GLYMA20G01360.1 (Glycine max), 3'ss of exon 3

------------------------------------------------------gtttgtgaattgttgctaatttgatgatcagattg-atgctgcagttcatttaagttatggtggtattaagcaatattctac---cgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACGTATGCACTTTTGGTGCTCTTCTGGGCCGAGATTTATTATCAG
                                                         |  || | |||| | |||  ||  ||  |||  |   ||  |||    | | ||||  |        ||  |    ||     | ||||  |||||||  |  | || ||  ||||  | || || ||||| || || |||||||||||||| || |||||||| ||||||||||| |||
gtattcgagtatagggaatggaatgtaacatttaatttgagtaattttttcttaacataagattagttgtttatt--attttctaattacagaatgtggttagactcacttatactcactcactgcgtttgcttatgtttgtagATTGTTCAACACATCTTGCTTGACGTGCCGAGTCTTGCTTTCTTTACAACATATGCACTTTTGGTCCTGTTCTGGGCTGAGATTTATTACCAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|29664023|gb|CB660298.1|CB660298

EST:     AAATGTGCAGCTCATACATCCAGAG                         ATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACG
genomic: AAATGTGCAGCTCATACATCCAGAGgtttgtgaat ... tctaccgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACG

EST: gi|218501789|gb|FG963428.1|FG963428

EST:     GATCCATAGTGTTTGTGCTGCGACGAAATGTGCAGCTCATACATCCAGAG                         ATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACG
genomic: GATCCATAGTGTTTGTGCTGCGACGAAATGTGCAGCTCATACATCCAGAGgtttgtgaat ... tctaccgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACG

EST: gi|29663945|gb|CB660220.1|CB660220

EST:     ACGAAATGTGCAGCTCATACATCCAGAG                         ATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACG
genomic: ACGAAATGTGCAGCTCATACATCCAGAGgtttgtgaat ... tctaccgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACG

EST: gi|218494016|gb|FG950655.1|FG950655

EST:     GATCCATAGTGTTTGTGCTGCGACGAAATGTGCAGCTCATACATCCAGAG                         ATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACG
genomic: GATCCATAGTGTTTGTGCTGCGACGAAATGTGCAGCTCATACATCCAGAGgtttgtgaat ... tctaccgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACG

EST: gi|218483378|gb|FG949318.1|FG949318

EST:     GATCCATAGTGTTTCCCCTGCGACGAAATGTGCAGCTCATACATCCAGAG                         ATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACG
genomic: GATCCATAGTGTTTGTGCTGCGACGAAATGTGCAGCTCATACATCCAGAGgtttgtgaat ... tctaccgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 53 (upstream exon), 74 (downstream exon)
Score: 64

TGCGATCCATAGTGTTTGTGCTGCGACGAAATGTGCAGCTCATACATCCAGAGgtttgtgaat ... tctaccgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACGTATGCACTTTTGGTGCTCTTCTGGGCCGAGATTTATTATCAG

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atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

attgatgctgcagttcatttaagttatggtggtattaagcaatattctaccgcagATACTTCAACATGTGCTTCTAGATATGCCCGGGCTCGCATTCTTCACGACGTATGCACTTTTGGTGCTCTTCTGGGCCGAGATTTATTATCAG