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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tccccttaaaatctggttcgttgttatattattttatttttcgttgctggtggcaataattgactttacaaaccatggggaatgttttgggttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGAGCTCCATGATCTTCTGAGCAAGCCTTCTTTGACTGGGATCCCATTACTA

Basic information

species Oryza sativa
transcript LOC_Os03g10370.1
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os03g10370.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GRMZM2G081622_T01 (Zea mays), 3'ss of exon 3
tccccttaaaatctggttcgttgttatattattttatttttcgttgctggtggcaa--taattgactttacaaacc----atggggaatgttttgg-------gttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGAGCTCCATGATCTTCTGAGCAAGCCTTCTTTGACTGGGATCCCATTACTA
             || | | |  || | ||| | |     |||| |||| | | ||   |||      |   |||      ||    ||||||          ||| ||||||||||||||| || || || |||||||| |||||||| ||||| ||  | || |||||||| ||  |||||||||| |||||||| ||||| || ||||||
-------------tgctgcatgattttcttaatctgaaggtcgtagctgatagaaaatcaataaggaatgagaacttctagtgtcagatgtttaaattgttttgtttctgcagGTATGTTGTTGACGCGGCAGATCGAGAAAACATGGCCATTGCAAAGGGGGAACTCCATGACCTCTTGAGCAAGCCATCTTTGACCGGGATTCCGTTACTA

upper sequence: LOC_Os03g10370.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GRMZM2G007188_T01 (Zea mays), 3'ss of exon 3
tccccttaaaatctggttcgttgttatattattttatttttcgttgctggtggcaa--taattgactttacaaaccatgggga---atgtt-ttgggttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGAGCTCCATGATCTTCTGAGCAAGCCTTCTTTGACTGGGATCCCATTACTA
      | ||| ||| | | |     | ||||| |     |||| |||| |   ||   |||      | | |    |||  |   || || ||  ||| ||||||||||||||| || || || ||||| || |||||||| ||||| ||| | ||||||||||||||  |||||||||| |||||||| || || |||||||||
------tgaaacctgctgcatgaccttcttattctgaaggtcgtagctgatacaaaatcaataagaacttctagtgttggatatttatattattttgtttctgcagGTATGTTGTTGACGCGGCAGATCGTGAAAACATGGCCATTGCAAAAGGGGAGCTCCATGATCTCTTGAGCAAGCCATCTTTGACCGGAATACCATTACTA
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|38470884|gb|CF955015.1|CF955015
EST:     AGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGT                         GTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
genomic: AGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGTgtaagttcct ... gttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
EST: gi|163920353|gb|EG710600.1|EG710600
EST:     AGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGT                         GTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
genomic: AGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGTgtaagttcct ... gttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
EST: gi|117230769|gb|CT854061.1|CT854061
EST:     AGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGT                         GTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
genomic: AGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGTgtaagttcct ... gttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
EST: gi|88202578|gb|CI200933.1|CI200933
EST:     AGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGT                         GTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
genomic: AGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGTgtaagttcct ... gttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
EST: gi|88962532|gb|CI097684.1|CI097684
EST:     GCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGT                         GTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
genomic: GCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGTgtaagttcct ... gttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
EST: gi|58680551|gb|CK069238.1|CK069238
EST:     AGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGT                         GTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA
genomic: AGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGTgtaagttcct ... gttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 70 (upstream exon), 62 (downstream exon)
Score: 36

TGACCAAAGGTAATGTCACAATAAAATTGTGGGATCTTGGAGGCCAGCCAAGATTCCGTAGCATGTGGGAGAGATACTGTCGTGCAGTTTCTGCAATTGTgtaagttcct ... gttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGAGCTCCATGATCTTCTGAGCAAGCCTTCTTTGACTGGGATCCCATTACTA




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gctggtggcaataattgactttacaaaccatggggaatgttttgggttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGAGCTCCATGATCTTCTGAGCAAGCCTTCTTTGACTGGGATCCCATTACTA
            aattgac  putative branch site (score: 36)
                                              ttcctgc  putative PPT
         aataattgacttta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tccccttaaaatctggttcgttgttatattattttatttttcgttgctggtggcaataattgactttacaaaccatggggaatgttttgggttcctgcagGTATGTTGTGGATGCAGCCGATCGAGAGAACATGGCTATTGCCAAAAGCGAGCTCCATGATCTTCTGAGCAAGCCTTCTTTGACTGGGATCCCATTACTA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCAAG
- - - - - - ctggttc
- - - - - - - - - -gttgtta
- - - - - - - - - - - - - - - - - - - - - gttgctg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -aaaccat
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tgttttg