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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaattcagcatccaactgaaacattttgtcagtggtaaaagaaaagcttcaataactcataactcaaattgtatgatgaattgatgactttacacatcataagactgacatgttcccatgataactccaattcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGCAGAGGAAGGAACATTCCTTGGTATCGAGGCTGCACTTGCAGGTTCACAG

Basic information

species Oryza sativa
transcript LOC_Os02g52650.1
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os02g52650.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GRMZM2G103101_T02 (Zea mays), 3'ss of exon 3
gtaattcagcatccaactgaaacattttgtcagtggtaaaagaaaagcttcaataactcataactcaaattgtatgatgaattgatgactttacacatcataagactgacatgttcccatgataactccaattcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGCAGAGGAAGGAACATTCCTTGGTATCGAGGCTGCACTTGCAGGTTCACAG
                                                     |||  |  |||  |||    |   |||  || ||| || |    ||||   |   |       |||  |||        ||   |||| || |||||||||||||||||||||||||| |  ||||||||||| |||||||||||||| |||||| | ||  | ||||||||||| ||||||  ||||
----------------------------------------------------gtaaacctcaacctaaaca--actgtgacctg-tgatttgatt--tcatggaa---attcaactccaatata--------tcatgacagGTTCGCTGAAACCAAGAGGTACATGGACTTCAAAAATCCAGGATCGCAAGCAGAGGAAGGGACATTCATAGGGTTAGAGGCTGCACTCGCAGGTCAACAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|8859261|gb|AU096579.1|AU096579
EST:     GA-TTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGG                         ATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
genomic: GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
EST: gi|38475135|gb|CF959268.1|CF959268
EST:     GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGG                         ATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
genomic: GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
EST: gi|88988697|gb|CI039116.1|CI039116
EST:     GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGG                         ATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
genomic: GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
EST: gi|33673864|gb|CF302103.1|CF302103
EST:     GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGG                         ATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
genomic: GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
EST: gi|88508359|gb|CI435865.1|CI435865
EST:     TAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGG                         ATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
genomic: TAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
EST: gi|33683439|gb|CF311678.1|CF311678
EST:     GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGG                         ATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
genomic: GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
EST: gi|88847374|gb|CI011317.1|CI011317
EST:     ACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGG                         ATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
genomic: ACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
EST: gi|58590186|gb|CF988494.1|CF988494
EST:     GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGG                         ATTTGCTGAAACCAAGAGGTACATGGACTTTATCAA
genomic: GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAA
EST: gi|86521070|gb|CI161276.1|CI161276
EST:     GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGG                         ATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
genomic: GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
EST: gi|33683440|gb|CF311679.1|CF311679
EST:     GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGG                         ATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC
genomic: GACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 75 (upstream exon), 71 (downstream exon)
Score: 36

CAGGAATCAACAACCTGCCAGTGTGGTTTGAAGCAGGTGCAACAAAATTCGACTTCGCTAACACCACGGCACTCTTCTTTGTCCAGCTTCTCTTGATGGGgtaattcagc ... ttcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGCAGAGGAAGGAACATTCCTTGGTATCGAGGCTGCACTTGCAGGTTCACAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gactttacacatcataagactgacatgttcccatgataactccaattcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGCAGAGGAAGGAACATTCCTTGGTATCGAGGCTGCACTTGCAGGTTCACAG
                                 tgataac  putative branch site (score: 36)
                                             ttccc  putative PPT
          atcataa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaattcagcatccaactgaaacattttgtcagtggtaaaagaaaagcttcaataactcataactcaaattgtatgatgaattgatgactttacacatcataagactgacatgttcccatgataactccaattcccaacagATTTGCTGAAACCAAGAGGTACATGGACTTTATCAATCCAGGATCACAAGCAGAGGAAGGAACATTCCTTGGTATCGAGGCTGCACTTGCAGGTTCACAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAA