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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tttgaaatgtcctgttcctcaccatgcttgaaaagttgaaacggaaagccacatctaagtaatcctttactgaactgcaacctttctctgcttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTTGCTGAGAAAGACATACTTCATGAAATGGTGAAAATAGTCAAGAAAAGG

Basic information

species Oryza sativa
transcript LOC_Os02g17350.1
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os02g17350.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GRMZM2G053925_T01 (Zea mays), 3'ss of exon 2
--------tttgaaatgtcctgttcctcaccatgcttgaaaagttgaaacggaaagccacatctaagtaatcctttactgaactgcaacctttctctgcttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTTGCTGAGAAAGACATACTTCATGAAATGGTGAAAATAGTCAAGAAAAGG
         ||||| ||  ||| | |   || | || ||||  |||   | ||   |||||| ||| ||| ||||||||||| | || | ||  ||  |||| ||||||| |||||||||||||| ||||| ||||||||| |||| ||||||   |||||||||| ||||||||||||||| ||||| || ||||| |||||||||
taggagaccttgaactggtctggtgcaagccctactggaaa--ttg---ctga---ccacatttaaataaccctttactgaattacagcattgttcgacttgtttcagTTGCTGGAGACGATGATAAAAAATTGTGGGGACGTTGTGCATATGGTTGTTGCTGAGAGAGACATACTTCATGAGATGGTCAAGATAGTAAAGAAAAGG

upper sequence: LOC_Os02g17350.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GLYMA06G08710.1 (Glycine max), 3'ss of exon 2
tttgaaatgtcctgttcc-tcaccatgcttgaaaagttgaaacggaaagccacatctaagtaatcctttactgaactgcaacctttctctgct-tgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTTGCTGAGAAAGACATACTTCATGAAATGGTGAAAATAGTCAAGAAAAGG
|  | |   | || |    ||  | |      ||| |         |      ||    | ||    | | |    | | |  |||  ||||| |  || || | |||||||| || || || || |||||||||||| |||| ||||| ||||| |||| |||  | |||||||| |||||||||||||| ||||| | |
tacggaggattctatctagtctgcgtataaagaaaatgtctgtatcatctttaataattggaaaggctcatttgttttcga--tttgactgctatattttagCTGCTGGAGACAATCATAAAGAATTGTGGGGACATTATCCACATGCATGTTGCCGAGAGAGATGTTCTTCATGAGATGGTGAAAATAGTAAAGAAGAAG

upper sequence: LOC_Os02g17350.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GLYMA17G26440.2 (Glycine max), 3'ss of exon 2
tttgaaatgtcctgttcctcacc-----atgcttgaaaagttgaaacggaaagccacatctaagtaatcctttactgaactgcaacctttctctgcttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTTGCTGAGAAAGACATACTTCATGAAATGGTGAAAATAGTCAAGAAAAGG
  | | |||   | |  || | |     ||  || |   ||  | |      |     |  ||  | || |||  |   |||         | |  |  |    |||  ||||||| || || || |||||||| || ||||| || ||||| ||||| || | |||  | |||||||| |||||||||||||| ||||||| |
-ctaagatgcattctctctgatctgcatataatttacttgtctagatcatttgatgatttgaaaaattcatttggttttctgttggtccattgtattaaa----gTTGTTGGAGACAATCATAAAGAACTGTGGAGATATTGTTCACATGCATGTTGCAGAAAGAGATGTGCTTCATGAGATGGTGAAAATAGTAAAGAAAAAG

upper sequence: LOC_Os02g17350.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GLYMA04G08600.1 (Glycine max), 3'ss of exon 2
tttgaaatgtcctgttcc-tcaccatgcttgaaaagttgaaacggaaagccacatctaagtaatcctttactgaactgcaacctttctctgct-tgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTTGCTGAGAAAGACATACTTCATGAAATGGTGAAAATAGTCAAGAAAAGG
   | |   | ||||    ||    |      ||| ||        |      ||    |  |    | | |    | | |  |||  ||||| |  || || | |||||||| || || || || ||||||||||||||||| ||||| ||||| |||| |||  | |||||||| |||||||||||||| ||||| | |
cagggaggattctgtctagtctgtgtataaagaaaatttctgtatcatctttaataattgagaaagctcatttgttttcca--tttgactgctatattttagCTGCTGGAGACAATCATAAAGAATTGTGGGGACATTGTCCACATGCATGTTGCCGAGAGAGATGTTCTTCATGAGATGGTGAAAATAGTAAAGAAGAAG

upper sequence: LOC_Os02g17350.1 (Oryza sativa), 3'ss of exon 3
lower sequence: Vv04s0008g03720.t01 (Vitis vinifera), 3'ss of exon 2
-tttgaaatgtcctgttcctcaccatgcttgaaaagttgaaacggaaagccacatctaagtaatcctttactgaactgcaacctttctctgcttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTTGCTGAGAAAGACATACTTCATGAAATGGTGAAAATAGTCAAGAAAAGG
  || |  |||    ||| |        |||     ||     |     |   |||| |   |   || | |    |    | ||| |||   |  |  || || |||||||  |  | ||||||||||| || |||||||||||||| || || |||| ||| || |||||||| |||||||||||||| ||||||| |
ccttcagttgtagatttcattcaatcaattgttgcattctcctgcttgcctttatctgatagaggattaaattggtt-ttgcttttgtctttctactatagCTATTGGAGACAGTCGTAAAAAACTGTGGAGATATTGTCCATATGCATGTAGCAGAGAGAGATATTCTTCATGAGATGGTGAAAATAGTAAAGAAAAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|88761841|gb|CI771397.1|CI771397
EST:     AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACG                         TTACTGGAGACGATGATCAAAAACTGTGGGG
genomic: AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACGgtgagtataa ... cttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGG
EST: gi|86901767|gb|CI310529.1|CI310529
EST:     AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACG                         TTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTT
genomic: AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACGgtgagtataa ... cttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTT
EST: gi|88399187|gb|CI618377.1|CI618377
EST:     AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACG                         TTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAA
genomic: AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACGgtgagtataa ... cttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAA
EST: gi|29667653|gb|CB663928.1|CB663928
EST:     AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACG                         TTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTT
genomic: AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACGgtgagtataa ... cttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTT
EST: gi|163925682|gb|EG710847.1|EG710847
EST:     AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACG                         TTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTT
genomic: AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACGgtgagtataa ... cttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTT
EST: gi|88419305|gb|CI599138.1|CI599138
EST:     AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACG                         TTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTT
genomic: AGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACGgtgagtataa ... cttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 67 (upstream exon), 60 (downstream exon)
Score: 15

GCAAGCAAAAGATGTCGTGAAGTCTATCAAGAAACGAATTGCACACAGGAATGCGAAGGTCCAGCTTCTTGCTCTCACGgtgagtataa ... cttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTTGCTGAGAAAGACATACTTCATGAAATGGTGAAAATAGTCAAGAAAAGG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aagccacatctaagtaatcctttactgaactgcaacctttctctgcttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTTGCTGAGAAAGACATACTTCATGAAATGGTGAAAATAGTCAAGAAAAGG
                                            gcttgat  putative branch site (score: 15)
                                   cctttctctgctt  CT-rich tract
          taagtaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tttgaaatgtcctgttcctcaccatgcttgaaaagttgaaacggaaagccacatctaagtaatcctttactgaactgcaacctttctctgcttgattcagTTACTGGAGACGATGATCAAAAACTGTGGGGACATTGTCCATATGCAAGTTGCTGAGAAAGACATACTTCATGAAATGGTGAAAATAGTCAAGAAAAGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAA
- - - -tgtcctg
- - - - - - - - - - - -atgcttg
- - - - - - - - - - - - - - - - - - - -aacggaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tttactg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - aactgca