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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tgttcctttatttgtctgtttatctgtgtttacatatttggtcatcagcaacataatcttagatatcgtttgatgacatttgatatttccttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGATCTTCAGAACGATGAGGATCAGGCGTACCGTAAGATCAGACTACGTGCCG

Basic information

species Oryza sativa
transcript LOC_Os12g21798.1
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os12g21798.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GLYMA07G10920.1 (Glycine max), 3'ss of exon 2
---------------------------------------tgttcctttatttgtctgtttatctgtgtttacatatttggtcatcagcaacataatctta--gatatcgtttgatgacatttgatat--ttccttcattttag-
                                        |||  |  || | ||   ||  || | ||||    |||  |  |  |  |  |  |  ||   |  | || || | |  |    |||  ||  || |  | |   |||||||  || || ||||| ||  | ||||||||||||||||||| |||||||||    ||||||||||| || | |   ||||| ||  |  | || |                          
gtgtgtgcttattgcattttgtttctatgggagaacgagggtttatgaatctttcaagttgcctatttttatgggttttatggtatgtgatttggtgatatgaactttgtatgcttatttagactatggttgtttgaaatgacttattgcttatgaaggactcaaacatagAGTGTTTGAGGTCTCCTTGGTTGATCTTCAAGGAGATGAGGATCATGCATTCAAGAAGATTAGGTTGAGAGCTGAAGATGTGCAAGGGAAAAATGTTCTG

upper sequence: LOC_Os12g21798.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GLYMA06G11940.1 (Glycine max), 3'ss of exon 2
----tgttcctttatttgtctgtttatctgtgtttac-atatttggtcatcagcaacataatcttagatatcgtttgatgacat--ttgatatttccttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGATCTTCAGAACGATGAGGATCAGGCGTACCGTAAGATCAGACTACGTGCCG
    ||||    | ||||  | | ||   ||  || | |    || |  | |    |  |   ||| |||     || ||||||  | | |||||  |  |    ||| |||||| || || || || || || |||||||||||||| ||||||||||||||    |||||||| || || | |   |||||  |  |  | ||||
aatgtgttttactctttgaatttataatagtaattgctacgagtgatt-taaattgctgagctttatata---gatggtgacatgatggctattttttaaac---agACTGCTTCGGATGGACTAAAACATCGAGTGTTTGAGGTCTCATTGGCTGATCTTCAAGGTGATGAGGACCATGCATTCAAGAAGATTCGTTTGAGAGCCG

upper sequence: LOC_Os12g21798.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GLYMA10G39050.1 (Glycine max), 3'ss of exon 2
--tgttcctttatttgtctgtttatctgtgtttacatatttggtcatcagcaacataatcttagatatcgtttgatgacatttgatatttccttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGATCTTCAGAACGATGAGGATCAGGCGTACCGTAAGATCAGACTACGTGCCG
  | | | |    | |  | |||    |  | |     | ||    |  |    || | ||| | |||  |    |    | |||| | |     ||   |||||||||| || || ||||||||  | |||||||||||||||||||||||||||||    ||||||||||| || | |   ||||| ||  |  | || |
ggtctgcttactgtcgcttattttcaagcatattatggtgtgtgacttggtgttatgaactttg-tatgctcatgtagactgtgat-tgtttcaaataacagATTGCTTCTGAAGGACTCAAGCATAGAGTGTTTGAGGTCTCCTTGGCTGATCTTCAAGGGGATGAGGATCATGCATTCAAGAAGATTAGGTTGAGAGCTG

upper sequence: LOC_Os12g21798.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GLYMA20G28780.3 (Glycine max), 3'ss of exon 2
----tgttcctttatttgtctgtttatctgtgtttacatatttggtcatcagcaacataatcttagatatcgtttgatgacatttgatatttccttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGATCTTCAGAACGATGAGGATCAGGCGTACCGTAAGATCAGACTACGTGCCG
      |      | ||| || |||   || ||||     | ||    |  |  | || | ||| | ||| | ||  | | | | | | ||||    ||   |||||||||| || || || || ||  | |||||||||||||||||||||||||||||    ||||||||||| || | |   ||||| ||  |  | || |
taagaatcttccaagttgcctattttcatgggttttatggtgtgtgatttggtgatatgaactttg-tat-gcttatttagactggttctttcaa--atgacagATTGCTTCTGAAGGACTTAAACATAGAGTGTTTGAGGTCTCCTTGGCTGATCTTCAAGGGGATGAGGATCATGCATTCAAGAAGATTAGGTTGAGAGCTG

upper sequence: LOC_Os12g21798.1 (Oryza sativa), 3'ss of exon 2
lower sequence: Vv14s0030g01810.t01 (Vitis vinifera), 3'ss of exon 3
tgttcctttatttgtctgtttatctgtgtttacatatttggtcatcagca-acataatcttagatatcgtttgatga-catttgatatttccttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGATCTTCAGAACGATGAGGATCAGGCGTACCGTAAGATCAGACTACGTGCCG
  ||  ||||      | ||| |   | ||   | |      | |   ||  | |  | ||| |||| ||||  |||  || | || |    |   |   ||||||| || || || ||||||||| | ||||||||| | ||| |||||||||||||||||||||||||||| || ||| | |||||| ||||  | || |
-tttaatttaaggaaatatttgttcttattctaaaagaaaaacgtttacatgccttttattacatat-gtttactgaatatgttatgtgaattatgtgacagATTGCCTCTGAAGGGCTCAAGCACAGAGTGTTTGAGATTTCCCTGGCTGATCTTCAGAACGATGAGGATCATGCCTACAGGAAGATCCGACTGAGAGCTG

upper sequence: LOC_Os12g21798.1 (Oryza sativa), 3'ss of exon 2
lower sequence: PP1S17_306V6.1 (Physcomitrella patens), 3'ss of exon 2
-------------------------tgttcctttatttgtctgtttatctgtgtttacatatttggtcat--cagcaacataatcttagatatcgtttgatgacatttgatatttcct-----tcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGATCTTCAGAACGATGAGGATCAGGCGTACCGTAAGATCAGACTACGTGCCG
                         |  |  ||||||| |  | |       | ||   |  | |  |       | || |       |  |||| | | ||| |   | |  | | |     || | | ||||||| || || ||  | |||   |||||||| |||||||||||||||||| | ||||| || || |||||||| | ||| ||||| |  ||  | ||||
gtattgagggttgagttttttatttttattttttatttatgggatgggtgtcgctttattgctggagcgcgctgtccactttgctcacggaatcgatagctgataaaaggttctacttgatggtcttatcagATTGCCTCCGACGGGTTGAAGGGTCGTGTGTTCGAGGTCTCCTTGGCTGATTTGCAGAATGACGAAGATCAGGCTTTCCGCAAGATTAAGCTGAGGGCCG

upper sequence: LOC_Os12g21798.1 (Oryza sativa), 3'ss of exon 2
lower sequence: PP1S92_47V6.2 (Physcomitrella patens), 3'ss of exon 2
tgttcctttatttgtctgtttatctgtgtttacatatttggtcatca-gcaacataatcttagatatcgtttgatgacatttgatattt---ccttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGATCTTCAGAACGATGAGGATCAGGCGTACCGTAAGATCAGACTACGTGCCG
   |    ||||| |   ||||||    | |  || |||   || |  ||    |||    | |      ||| |||||| |  |  ||      |  | | ||||||| || || ||  | |||  |||||||||||||||||||||||||||| | || || || ||||| ||||| | ||| ||||| |  ||  | || |
caatttgctatttatgaatttatccagttatctatctttcagcaccttgccttctaa----aaacggtagttgttgacatgttgttcttgaatggtggtatcagATTGCCTCCGATGGTTTGAAGGGCCGTGTGTTTGAGGTCTCCTTGGCTGATTTGCAAAATGACGAGGACCAGGCTTTCCGCAAGATTAAGCTGAGGGCTG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|33795240|gb|CF323492.1|CF323492
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|58684406|gb|CK073093.1|CK073093
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|87006042|gb|CI289459.1|CI289459
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|117216849|gb|CT845233.1|CT845233
EST:     ACGTGAGAAACGGGGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|88352366|gb|CI615620.1|CI615620
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|88398529|gb|CI595930.1|CI595930
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|44675333|gb|CR288767.1|CR288767
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|58686699|gb|CK075386.1|CK075386
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|88396444|gb|CI600927.1|CI600927
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGGTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|87023682|gb|CI286433.1|CI286433
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|88390567|gb|CI609192.1|CI609192
EST:     CGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGA
genomic: CGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGC-ACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGA
EST: gi|33812109|gb|CF331944.1|CF331944
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTAAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|88388997|gb|CI595231.1|CI595231
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|218498217|gb|FG958345.1|FG958345
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|58670980|gb|CK059666.1|CK059666
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|88426821|gb|CI584184.1|CI584184
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|218490212|gb|FG960812.1|FG960812
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|38477979|gb|CF962112.1|CF962112
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGAGGCAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|88424522|gb|CI608108.1|CI608108
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|38473073|gb|CF957205.1|CF957205
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|218500733|gb|FG957743.1|FG957743
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
EST: gi|9884533|gb|BE607366.1|BE607366
EST:     ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAG                         ATTGCTTCAGATGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT
genomic: ACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 74 (upstream exon), 72 (downstream exon)
Score: 69

TCCTTTTGCGAAGAAGGATTGGTATGATATCAAGGCCCCGTCGGTGTTCAACGTGAGAAACGTCGGGAAGACGCTCGTGTCCAGGACACAGGGCACAAAGgtatcagcct ... ttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGATCTTCAGAACGATGAGGATCAGGCGTACCGTAAGATCAGACTACGTGCCG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cagcaacataatcttagatatcgtttgatgacatttgatatttccttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGATCTTCAGAACGATGAGGATCAGGCGTACCGTAAGATCAGACTACGTGCCG
                                atttgat  putative branch site (score: 69)
                                      tatttccttcatttt  CT-rich tract
       ataatcttagatat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgttcctttatttgtctgtttatctgtgtttacatatttggtcatcagcaacataatcttagatatcgtttgatgacatttgatatttccttcattttagATTGCTTCAGAGGGCCTCAAGCACCGTGTGTTTGAGGTCTCCTTGGCTGATCTTCAGAACGATGAGGATCAGGCGTACCGTAAGATCAGACTACGTGCCG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGATCA
- - - - - - - tctgttt
- - - - - - - - - - - - tgtgttt
- - - - - - - - - - - - - - - - - - - - - - - - caacata
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttgatga