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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...catgtttgacagtttgagagtccacctagctcctcttgtactgcttatataaaatattattaacttttactaagtgtctgtttcttcctcaatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGACTACCCAGAGAAGCCACCATCTGTCCGGTTTCATTCAAGAATCAATATGT

Basic information

species Oryza sativa
transcript LOC_Os09g12570.1
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os09g12570.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GRMZM2G056501_T01 (Zea mays), 3'ss of exon 2
------------------------------------------------catgtttgacagtttgagagtccacctagctcctcttgtactgcttatataaaatattattaacttttactaagtgtctgtttcttcctcaatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGACTACCCAGAGAAGCCACCATCTGTCCGGTTTCATTCAAGAATCAATATGT
                                                 || |||||| | | ||   |  || |  || |  || |  ||||||| | |  |  | |  | || || ||| | ||| |||||| ||  |      |||  |||||||| ||||||||||||||||||||| ||||||| |||||||||||||| ||||                                       
aatcaaatgtgcagcattttgcctcatgtttgtttacatgacacttgaaatctttgactgat-gaagctgtactttcctacatttcttttgcttatttcagTTTATGTCGAGTTGTATTAA-TCTCT-TTTCTTACTTGA------AGACCGTCCATGAGGGTCGCATCTACCAGCTGAAGTTGTTCTGCGACAAGGACTACCCTGAGA---------------------------------------

upper sequence: LOC_Os09g12570.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GRMZM2G056501_T02 (Zea mays), 3'ss of exon 2
---------catgtttgacagtttgagagtccacctagctcctcttgtactgcttatataaaatattattaacttttactaagtgtctgtttcttcctcaatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGACTACCCAGAGAAGCCACCATCTGTCCGGTTTCATTCAAGAATCAATATGT
          || |||||| | | ||   |  || |  || |  || |  ||||||| | |  |  | |  | || || ||| | ||| |||||| ||  |      |||  |||||||| ||||||||||||||||||||| ||||||| |||||||||||||| |||||||||||||| || || |||||||||||||| ||  |  
gacacttgaaatctttgactgat-gaagctgtactttcctacatttcttttgcttatttcagtttatgtcgagttgtattaa-tctct-tttcttacttga------agACCGTCCATGAGGGTCGCATCTACCAGCTGAAGTTGTTCTGCGACAAGGACTACCCTGAGAAGCCACCATCAGTTCGATTTCATTCAAGAATAAACTTAA

upper sequence: LOC_Os09g12570.1 (Oryza sativa), 3'ss of exon 2
lower sequence: PP1S490_20V6.1 (Physcomitrella patens), 3'ss of exon 3
-catgtttgacagtttgagagtccacctagctcctcttgtactgcttatataaaatattattaacttttactaagtgtctgtttcttcctcaatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGACTACCCAGAGAAGCCACCATCTGTCCGGTTTCATTCAAGAATCAATATGT
  |  | ||| |         ||    |   |  | | | |||   | ||     | ||| | | || |          | |      ||   |    |||   || ||||||||||| ||||||    |||| |||||||| |||||||||||||| || ||||||||| ||||  | || |||||  | ||||| ||| 
ttacttgtgataaaccccatttcattgtttttgatttcgcactctgtctaagttgtgttactgagttgtcaccgacacat-tcaagctcttgtttgtacagTCAGTTCATGAAGGTCGTATCTACACATTGAAACTGTTCTGCGACAAGGACTACCCCGATAAGCCACCAACTGTTAGATTCCATTCTCGGATCAACATGA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|88463486|gb|CI695950.1|CI695950
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|86897150|gb|CI294270.1|CI294270
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|88401056|gb|CI685038.1|CI685038
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|86599346|gb|CI251304.1|CI251304
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|2280993|gb|C25488.1|C25488
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|33659068|gb|CF281681.1|CF281681
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|55410529|gb|CV722905.1|CV722905
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|88361792|gb|CI681952.1|CI681952
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|10930784|gb|AU161058.1|AU161058
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|88349347|gb|CI678329.1|CI678329
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|55439494|gb|CV733056.1|CV733056
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|29644928|gb|CB649935.1|CB649935
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|88741058|gb|CI764566.1|CI764566
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
EST: gi|27548074|gb|CA767449.2|CA767449
EST:     ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAAT                         AGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC
genomic: ATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 73 (upstream exon), 70 (downstream exon)
Score: 87

TGAAAAGGGCATTGGAGATGGAACCGTGAGCTATGGAATGGATGATGCAGATGACATCTACATGCGGTCTTGGACTGGCACCATCATTGGTCCACATAATgtgagattat ... aatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGACTACCCAGAGAAGCCACCATCTGTCCGGTTTCATTCAAGAATCAATATGT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tatataaaatattattaacttttactaagtgtctgtttcttcctcaatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGACTACCCAGAGAAGCCACCATCTGTCCGGTTTCATTCAAGAATCAATATGT
            tattaac  putative branch site (score: 87)
                               tctgtttcttcctca  putative PPT
     aaaatattattaactt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
catgtttgacagtttgagagtccacctagctcctcttgtactgcttatataaaatattattaacttttactaagtgtctgtttcttcctcaatctggcagAGTGTCCATGAAGGTCGCATCTACCAGCTGAAGCTGTTCTGTGACAAGGACTACCCAGAGAAGCCACCATCTGTCCGGTTTCATTCAAGAATCAATATGT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGC
- tgtttga
- - - - - - - - - - - - - -agctcct
- - - - - - - - - - - - - - - - - - - - ctgctta