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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...agatgaaaccatacatataaaagacatacattgtagcagtaatgtaaatccgagactgaacttacctgtcaatctgttattatttttttctcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGTCGAAAG

Basic information

species Oryza sativa
transcript LOC_Os08g41460.8
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os08g41460.8 (Oryza sativa), 3'ss of exon 2
lower sequence: GRMZM5G885392_T01 (Zea mays), 3'ss of exon 3
agatgaaaccatacatataaaagacatacattgtagcagta-atgtaaatccgagactgaacttacctgtcaatc--tgttattatttttttctcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGTCGAAAG
     |  || ||    ||||   |  ||||  | |||  | | | | ||       |   ||||  |  ||||   | || ||  |  |||| | |  | ||||||| ||||| || || ||||| ||||||||||||||||| || || |||||||||
---aaattccctattactaaatatcccacatgattgcaccataagcatataaagtgttattcttaatttgcaattatttttcttgataatttcccctctctagAAAGAAGGAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|29625905|gb|CB630916.1|CB630916
EST:     ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGG                         AAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
genomic: ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGGgttggtcttt ... tcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
EST: gi|29683115|gb|CB679390.1|CB679390
EST:     ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGG                         AAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
genomic: ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGGgttggtcttt ... tcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
EST: gi|29684942|gb|CB681217.1|CB681217
EST:     ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGG                         AAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
genomic: ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGGgttggtcttt ... tcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
EST: gi|29662553|gb|CB658828.1|CB658828
EST:     ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGG                         AAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
genomic: ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGGgttggtcttt ... tcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
EST: gi|29663222|gb|CB659497.1|CB659497
EST:     ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGG                         AAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
genomic: ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGGgttggtcttt ... tcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
EST: gi|29615644|gb|CB620656.1|CB620656
EST:     ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGG                         AAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT
genomic: ACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGGgttggtcttt ... tcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 72 (upstream exon), 57 (downstream exon)
Score: 97

CCCTGATGAGCAAAGACTTTTGGAAGCATCCATGGCTTATGTGGCTGGCAACCCCATTATGACAGATGCTGAATTTGATGAACTAAAGCTGAGATTAAGGgttggtcttt ... tcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGTCGAAAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aaatccgagactgaacttacctgtcaatctgttattatttttttctcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGTCGAAAG
             aacttac  putative branch site (score: 97)
                           tctgttattatttttt  putative PPT
                       tcaatctgttattatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
agatgaaaccatacatataaaagacatacattgtagcagtaatgtaaatccgagactgaacttacctgtcaatctgttattatttttttctcttggccagAAAGAGGGAAGTGAAATCGTACAAGAAGGTCCAAGATGCAGTCTTCGAAGTCGAAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGATG