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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgcgagatccccaccaaaatctctcttcttttttgctactgtttttcgaattactagtatctgcttgaaaatatcatattttgattttgaatgaaaaagaaagatctcgtcttgtccttttgttttctcaccttgctttgctcttcttctgcttatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAGCTCCTCCAGGCCGACGCAAAT

Basic information

species Oryza sativa
transcript LOC_Os05g02130.1
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os05g02130.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GRMZM2G141922_T01 (Zea mays), 3'ss of exon 2
gtgcgagatccccaccaaaatctctcttcttttttgctactgtttttcgaattactagtatctgcttgaaaatatcatattttgattttga-atgaaaaagaaagatctcgtcttgtccttttgttttctcaccttgctttgctcttcttctgcttatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAGCTCCTCCAGGCCGACGCAAAT
||||| ||| ||  | |     | ||||  ||| | || |   |   | | | || || |||| |||     || |  | ||  ||      | | |  | ||| |||||  |||  ||       | |||   ||  ||||||||                ||||||||||||||||||||||| |||||||||||||||||||||| ||  ||||||||  |||||| ||| |||
gtgcgggatgccatcgacgccttttctttctttctcctccccctccgcaactcacaag-atct-cttttttttacc-cacttcaatcaaatcaaggaggacaaaaatctcacctttacccccc---tgctcgtttt--tttgctctatc-------------cagACGCCGCTGATGCTCGCGGCGATGCACGGCAAGATCGACTGCGCGCGGAAGCTCCTGGAGGCCGGCGCCAAT

upper sequence: LOC_Os05g02130.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GRMZM2G069162_T02 (Zea mays), 3'ss of exon 2
gtgcgagatccccaccaaaatctctcttcttttttgctactgtttttcgaattactagtatctgcttgaaaatatcatattttgattttgaatgaaaaagaaagatctcgtcttgtccttttgttttctcaccttgctttgctcttcttctgcttatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAGCTCCTCCAGGCCGACGCAAAT
                                                                                                    ||| ||||| |||           | |  |  |||||| ||||  | ||   ||     | ||||||||||||||||||||||| ||||||||||||| |||||||||||| |||||||| ||||||| ||| |||
--------------------------------------------------------------------------------------------------taaaaaatctcatctc----------tctaccggcttgctatgcttgtttttctctg----tccagacgccgctgatgctcgcggcgatgcacggcaagaccgactgcgtgctgAAGCTCCTGCAGGCCGGCGCCAAT

upper sequence: LOC_Os05g02130.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GRMZM2G069162_T04 (Zea mays), 3'ss of exon 2
gtgcgagatccccaccaa--aatctctcttcttttttgctactgtttttcgaattactagtatctgcttgaaaatatcatattttgattttgaatgaaaaagaa-------agatctcgtcttgtccttttgttt--------------------tctcaccttgct------ttgctcttcttctgcttatgc-tgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAGCTCCTCCAGGCCGACGCAAAT
||||  ||||||  || |   |    | || || |||  | ||   ||||      |   |  | ||| | ||  |||     | |  |   |     ||| |        |  | |  ||||  ||| ||   |                    ||||| ||  ||      ||||| | ||| |  || |   | ||||||||||||||||||||||| ||||||||||||| |||||||||||| |||||||| ||||||| ||| |||
gtgctggatcccgtcccatcgacgcttttttttctttctttcttcctttctttccgccctttgccgctcgcaaggatctctcgtgggctaccagcttcaaaaatctcacctattttttttcttaccctcttcaatcaaatcaacaaggtaaaaaatctcatctctctaccggcttgctatgcttgtttttctctgtccagACGCCGCTGATGCTCGCGGCGATGCACGGCAAGACCGACTGCGTGCTGAAGCTCCTGCAGGCCGGCGCCAAT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|58667172|gb|CK055858.1|CK055858
EST:     TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAG                         ACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAG
genomic: TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAGgtgcgagatc ... tatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAG
EST: gi|88367923|gb|CI572644.1|CI572644
EST:     TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGGACAAGCAG                         ACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGGGTGCTCAAG
genomic: TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAGgtgcgagatc ... tatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAG
EST: gi|88368292|gb|CI577563.1|CI577563
EST:     TGTTCTTGGATCGGGGGGCGCCGGCGGACGCGGTGAATCGGCACAAGCAG                         ACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGGTCAAG
genomic: TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAGgtgcgagatc ... tatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAG
EST: gi|86908937|gb|CI302648.1|CI302648
EST:     TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAG                         ACGCCGCTGATGCTCGCGGCCATGCA
genomic: TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAGgtgcgagatc ... tatgctgcagACGCCGCTGATGCTCGCGGCCATGCA
EST: gi|87011260|gb|CI289530.1|CI289530
EST:     TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAG                         ACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAG
genomic: TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAGgtgcgagatc ... tatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAG
EST: gi|87015462|gb|CI303068.1|CI303068
EST:     TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAG                         ACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAG
genomic: TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAGgtgcgagatc ... tatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAG
EST: gi|88403350|gb|CI573073.1|CI573073
EST:     TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATTGGCACAAGCAG                         ACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAG
genomic: TGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAGgtgcgagatc ... tatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 75 (upstream exon), 53 (downstream exon)
Score: 17

GTGCTCTCCATGTTCTTGGATCGCGGGGCGCCGCCGGACGCGGTGAATCGGCACAAGCAGgtgcgagatc ... tatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAGCTCCTCCAGGCCGACGCAAAT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gtcttgtccttttgttttctcaccttgctttgctcttcttctgcttatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAGCTCCTCCAGGCCGACGCAAAT
                ttctcac  putative branch site (score: 17)
       ccttttgttttctcac  CT-rich tract
         ttttgtttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgcgagatccccaccaaaatctctcttcttttttgctactgtttttcgaattactagtatctgcttgaaaatatcatattttgattttgaatgaaaaagaaagatctcgtcttgtccttttgttttctcaccttgctttgctcttcttctgcttatgctgcagACGCCGCTGATGCTCGCGGCCATGCACGGCAAGATCGACTGCGTGCTCAAGCTCCTCCAGGCCGACGCAAAT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTCCTC