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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttgtacttaaatttatctagaatacttattctcaaaattttagttttctttctccaaaaaggtggcattctaacttgacaaccaatttctacctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG

Basic information

species Oryza sativa
transcript LOC_Os03g52840.3
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os03g52840.2 (Oryza sativa), 3'ss of exon 5
lower sequence: GRMZM2G135283_T03 (Zea mays), 3'ss of exon 5
-ttgtacttaaattta--tctagaatac-ttattctcaaaattttagttttctttctccaaaaaggtggcattctaacttgacaaccaatttctacctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
    || |||  |||   |   ||  |  ||  ||||| ||| |||| |  ||||   | ||||  |    |  |||||    | | | |||| |||| |  ||||||||||||||||||||| || || || |||||||||||||| |||||||| || ||||| |||||||| || ||||||||||||||||||||||| ||
tcaatatttatcttttgtttctgaccatatttctctcataatgttagatgccttttcaccaaaattt----tcttaactcatgaccaattttcaacctttatagTGAATGTGCAACCTCTATCCGGTTCGCCCGCCAACTTCCATGTATACACTGCACTCTTGAAGCCACATGAAAGGATCATGGCTTTGGATCTTCCTCACGG

upper sequence: LOC_Os03g52840.2 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA14G36280.2 (Glycine max), 3'ss of exon 5
ttgtacttaaatttatctagaatacttattctcaaaattttagttttcttt-ctccaaaaaggtggcattctaacttgacaaccaatttctacctct--gcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
  || | ||  | | | |  | || ||     || |  ||  |    |     |   | || |||   |  |       |      | ||| | | |   |||| ||||||||| |  |||| || || ||| | |||||||| |||||||||||  |||||||||| ||||||||||| |||||  | |||||||| |||||
--gttcatatgtct-tttctattattttcctccacagcttgggggaacaaaattttgataatgtgctttcttggagaatcttatgttctctgcattttgacagTCAATGTGCAATCATTATCTGGATCCCCTTCTAACTTCCAAGTTTACACTGCTTTATTGAAACCTCATGAGAGAATTATGGCACTTGATCTTCCCCATGG

upper sequence: LOC_Os03g52840.2 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA08G37270.1 (Glycine max), 3'ss of exon 5
---------ttgtacttaaatttatctagaatacttattctcaaaattttagttttctttctccaaaaaggtggcattctaacttgacaaccaatttctacctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
             | ||   || || |  ||||  |    | ||| | |||| | | | | |           |  |||| |  || ||        |      ||||||||| ||||| ||||| |||||||| ||||| || ||||| |||||||||||  |  | ||||| ||||| |||||||||||  | ||||||||||||||
ataatcactgaagagttttcttgatgttcaatagatccattaaaagtattaggtgtttgttt---------aagtcttctgatatgtcactgttaccccttaattgcagTGAACGTGCAGCCTCTGTCAGGGTCTCCTGCAAATTTCCACGTTTACACTGCATTGCTTAAACCTCATGAAAGAATCATGGCACTTGATCTTCCTCATGG

upper sequence: LOC_Os03g52840.2 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA18G27710.1 (Glycine max), 3'ss of exon 6
--ttgtacttaaatttatctagaatactt---attctcaaaatttt--agttttctttctccaaaaaggtggcattctaacttgacaaccaatttctacctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
  |        |  || |  |   | ||    ||||   |||||||  | |    ||| |||||       |  |||| |  || ||        |      ||||||||| ||||| ||||| |||||||| ||||| || |||||||| ||||||||  |  | ||||| ||||| |||||||||||  | ||||||||||||||
aatcacttaagagctttttcaatgtcctatagattcattaaattttgaaataaattttgtccaa-------gtcttctgatatgccactgttaccccttaattgcagTGAACGTGCAGCCTCTGTCAGGGTCTCCTGCAAATTTCCATGTCTACACTGCATTGCTTAAACCTCATGAAAGAATCATGGCACTTGATCTTCCTCATGG

upper sequence: LOC_Os03g52840.2 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA02G38160.2 (Glycine max), 3'ss of exon 5
ttgtacttaaatttatctagaatacttattctcaaaattttagttttcttt-ctccaaaaaggtggcattctaacttgacaaccaatttctacctct--gcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
  || | ||  | | | |  | || ||     || || ||  |    |     |   | || |||   |  |       |      | ||| | | |   |||| ||||||||| |  |||| || || ||| | |||||||| |||||||||||  |||||||||| ||||||||||| |||||  | |||||||| |||||
--gttcatatgtct-tttctattattttcctccacaacttgggggaacaaaattttgagaatgtgctttcttggagaatcttatgttctctgctttttgacagTCAATGTGCAATCATTATCTGGATCCCCTTCTAACTTCCAAGTTTACACTGCTTTATTGAAACCTCATGAGAGAATTATGGCACTTGATCTTCCCCATGG

upper sequence: LOC_Os03g52840.2 (Oryza sativa), 3'ss of exon 5
lower sequence: AT4G37930.1 (Arabidopsis thaliana), 3'ss of exon 5
ttgtacttaaatttatctagaatacttattctcaaaattttagttttctttctccaaaaaggtggcattctaact-------tgacaaccaattt--ctacctct-gcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
                                 ||| |  || |||  | | |   | ||    | | ||| | |       |||   |  ||||  ||   |||  |||||||||| |||||| | || || || ||||||||||||||||| ||||||||  | || || || ||||| |||||||||||  | ||||||||||||||
-------------------------------gtaaattactactttcatgtatatgagaaacaagaaatctgattagtcctttgatggcttatttgactggatcttacagTGAATGTTCAACCTTTGTCTGGATCTCCTGCCAACTTCCATGTGTACACTGCATTGTTAAAGCCTCATGAAAGAATCATGGCACTTGATCTTCCTCATGG

upper sequence: LOC_Os03g52840.2 (Oryza sativa), 3'ss of exon 5
lower sequence: Vv00s0211g00120.t01 (Vitis vinifera), 3'ss of exon 5
ttgtacttaaatttatctagaatacttattctcaaaattttagttttctttctccaaaaaggtggcattctaacttgacaaccaatttc---tacctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
     |      || |||    | ||| ||       |  |  |||||       || |  ||| ||   |   |  |     |||  |   |   | ||||||||| |||||  || |||| || || ||||| || || || ||||||||||| ||||| ||| | ||||| ||||| |||||  | ||||||||||||||
ccaggcgctgtgttttct---cttcttctttctcctttcctttttttcagcaggtaatagtgtgtcagctttgataaatgtttaatcacccttggttatgcagTGAACGTGCAGTCTTTATCTGGCTCGCCTGCAAATTTTCAAGTTTACACTGCACTATTAAAAGCTCATGAAAGAATTATGGCACTTGATCTTCCTCATGG

upper sequence: LOC_Os03g52840.2 (Oryza sativa), 3'ss of exon 5
lower sequence: Vv18s0001g07960.t01 (Vitis vinifera), 3'ss of exon 5
ttgtacttaaat---ttatctagaatacttattctcaaaattttagttttctttctccaaaaaggtggcattctaa-cttgacaaccaatttctacctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
 | |  ||||||   |||||||   |      || ||       ||   | |||||  | |        |||  || |||    |   |||| |   |  ||||| ||||||||  |  |||| || || ||||||||||| || | |||||| ||  |||| ||||| |||||||||||||||||  | ||||||||||||||
-tttgtttaaatcatttatctatt-tgtccgctcccacgtaaagagacatatttctgaacaccaactttatttaaagcttatttatttattttt--tttggcagTCAATGTGCAGTCATTATCTGGATCCCCTGCCAACTTTCAAGCTTACACGGCATTATTAAAACCTCATGAGAGAATCATGGCACTTGATCTTCCTCATGG

upper sequence: LOC_Os03g52840.2 (Oryza sativa), 3'ss of exon 5
lower sequence: Vv00s0211g00100.t01 (Vitis vinifera), 3'ss of exon 5
ttgtacttaaatttatctagaatacttattctcaaaattttagttttctttctccaaaaaggtggcattctaacttgacaaccaatttctacctct---gcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
 |||  ||    || |      | |||  ||      |  |  |||||    |  || |  ||| ||   |   |  |     |||  |    | |   |||||||| |||||  || |||| || || ||||| || || || ||||||||||| ||||| ||||| ||||| ||||| |||||  | ||||||||||||||
ctgtgttttctcttcttcttctttctttctcctt---tcctttttttcagcatgtaatagtgtgtcagctttgataaatgtttaatcaccctttgttatgcagTGAACGTGCAGTCTTTATCTGGCTCGCCTGCAAATTTTCAAGTTTACACTGCACTATTAAAACCTCATGAAAGAATTATGGCACTTGATCTTCCTCATGG

upper sequence: LOC_Os03g52840.2 (Oryza sativa), 3'ss of exon 5
lower sequence: PP1S21_270V6.1 (Physcomitrella patens), 3'ss of exon 5
--------------------------------------ttgtacttaaatttat---ctagaatacttattctcaaaattt-----tagttttctttctccaaaaaggtggcattctaacttgacaaccaatttctacctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
                                       |||  | ||||   |   ||  | | |   | ||    | |       ||| ||| |  |    | || || |  ||   ||  ||   |||  |||   |   ||||||| |||||  | || || || || || || ||||| || || ||||| || ||  |||| ||||||||| | |||||||| |||||||||||||||||
gtcagtcctctctgcttatgcatactcagttaccgaatctgttttcaaatacgtttgctgaagttcccttcctgcttactcggtagcagtattcgtcttttctagagatgcctatcatgctc-actttcaacgtctttttgaacagTGAACGTGCAGTCCCTGTCTGGATCGCCAGCAAACTTTCAGGTGTACACAGCTCTCCTGAAGCCACATGAGCGGATCATGGCGTTGGATCTTCCTCATGG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|25798315|gb|CA754276.1|CA754276
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|58689542|gb|CK078229.1|CK078229
EST:     CAGAAACGTGCTTTGGAGGCCTTCCGCTTGTGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CAGAAACGTGCTTTGGAGGCCTTCCGCTTG-GACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|58661735|gb|CK050415.1|CK050415
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|58691585|gb|CK080272.1|CK080272
EST:     CCAGAAACGTGC-TTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|29681406|gb|CB677681.1|CB677681
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|14571290|gb|BI118658.1|BI118658
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|38472219|gb|CF956350.1|CF956350
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|29674038|gb|CB670313.1|CB670313
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|33800499|gb|CF326119.1|CF326119
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|29681405|gb|CB677680.1|CB677680
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|29672938|gb|CB669213.1|CB669213
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|84117692|gb|CD285458.1|CD285458
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|33381615|gb|CF196958.1|CF196958
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|4714773|gb|AU055890.1|AU055890
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|88413328|gb|CI581343.1|CI581343
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|29674139|gb|CB670414.1|CB670414
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|58609394|gb|CK037427.1|CK037427
EST:     CAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
EST: gi|11442623|gb|BF430526.1|BF430526
EST:     CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAG                         TGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG
genomic: CCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 74 (upstream exon), 73 (downstream exon)
Score: 285

ATACATTGATATGGCCGAGTCATTGTGCCAGAAACGTGCTTTGGAGGCCTTCCGCTTGGACCCAGCGAAATGGGGAGgtaaatcatt ... acctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttctttctccaaaaaggtggcattctaacttgacaaccaatttctacctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG
                                        tttctacctct  CT-rich tract
                                      aatttcta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttgtacttaaatttatctagaatacttattctcaaaattttagttttctttctccaaaaaggtggcattctaacttgacaaccaatttctacctctgcagTGAATGTGCAACCTCTATCAGGGTCACCTGCCAACTTCCATGTTTACACTGCCCTATTGAAACCACATGAGAGAATCATGGCTTTGGATCTTCCTCATGG

- - - - - - - - - - - - - - - ctcaaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ggcattc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - caaccaa