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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gtttcagtcatatgatatgctatatgactctttcttatttttatatgttatatattagagaagtggagataaataaactcatcctgaaactactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGAATGGATTGACTACTTTATTTTGTCATCTTGTACAAGCATCTTATTGCGG

Basic information

species Oryza sativa
transcript LOC_Os05g38150.2
intron # 19
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|86851167|gb|CI312568.1|CI312568
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGG---                         -TCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86600986|gb|CI356743.1|CI356743
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88261913|gb|CI365489.1|CI365489
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|89187311|gb|CI047378.1|CI047378
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGG---                         -TCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86431434|gb|CI085045.1|CI085045
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88843208|gb|CI372108.1|CI372108
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86892380|gb|CI332315.1|CI332315
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88270030|gb|CI381328.1|CI381328
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|58662806|gb|CK051492.1|CK051492
EST:     GATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86872937|gb|CI326934.1|CI326934
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|66915778|gb|CX102626.1|CX102626
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86843774|gb|CI336595.1|CI336595
EST:     GACAAGGATGTCGTGTACACCCATAAGAATCTTCCTTTGCAATGAGGCAA                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88253457|gb|CI352083.1|CI352083
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88253863|gb|CI352489.1|CI352489
EST:     ACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAAATGAGG---                         -TCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCACTTTTAA
genomic: ACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAA-TGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATC-CTTTTAA
EST: gi|88485232|gb|CI422503.1|CI422503
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|25798187|gb|CA754084.1|CA754084
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGG---                         -TCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88411696|gb|CI521419.1|CI521419
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88304629|gb|CI520484.1|CI520484
EST:     GACAAGGATTTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88262625|gb|CI366399.1|CI366399
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGG---                         -TCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88274920|gb|CI388378.1|CI388378
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86841880|gb|CI337513.1|CI337513
EST:     GACAAGGATTTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         TTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86887353|gb|CI313256.1|CI313256
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86868617|gb|CI332994.1|CI332994
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTGTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|89178281|gb|CI391460.1|CI391460
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGG---                         -TCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|87029670|gb|CI257465.1|CI257465
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGG---                         -TCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88272298|gb|CI386752.1|CI386752
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86844715|gb|CI338654.1|CI338654
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|89193653|gb|CI116286.1|CI116286
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86846262|gb|CI339853.1|CI339853
EST:     ACCAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: A-CAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88266038|gb|CI376940.1|CI376940
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTAAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88977866|gb|CI384348.1|CI384348
EST:     GACAAGGATGTCGTTTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88262188|gb|CI365764.1|CI365764
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCC-TTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTTTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86889316|gb|CI317116.1|CI317116
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88262529|gb|CI366303.1|CI366303
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTCTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88382250|gb|CI533454.1|CI533454
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86425184|gb|CI070281.1|CI070281
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGGGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|86833055|gb|CI333654.1|CI333654
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|89196048|gb|CI395466.1|CI395466
EST:     GAC-AGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGA
EST: gi|88252559|gb|CI350987.1|CI350987
EST:     GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGA-G---                         GTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCACTTTTAAG
genomic: GACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATC-CTTTTAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 72 (upstream exon), 75 (downstream exon)
Score: 16

GATCTTGCGAGGGCGTGGGCAAGTGGTGAATGGTGACAAGGATGTCGTGTACACCCATAAGAGTCTTCCTTTGCAATGAGGCAAgtaatacact ... tactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGAATGGATTGACTACTTTATTTTGTCATCTTGTACAAGCATCTTATTGCGG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgttatatattagagaagtggagataaataaactcatcctgaaactactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGAATGGATTGACTACTTTATTTTGTCATCTTGTACAAGCATCTTATTGCGG
                                            ctacttt  CT-rich tract
                       ataaataaactcat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtttcagtcatatgatatgctatatgactctttcttatttttatatgttatatattagagaagtggagataaataaactcatcctgaaactactttgcagGTCAAATGCTCCTTTTAGCCTGTTCAGGAGTAGGTGAATATCCTTTTAAGAATGGATTGACTACTTTATTTTGTCATCTTGTACAAGCATCTTATTGCGG

- - - - - - - -tatgcta
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - aaataaa