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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...aacttggaagcagatgttaaccaggctgtaagcatgagtaattatagctgttgatacattctgaaaccattgaaattcagggctaagagatttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGCAAGATTGGTGATGCTGGCCAAAAG

Basic information

species Oryza sativa
transcript LOC_Os09g39590.1
intron # 16
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os09g39590.1 (Oryza sativa), 3'ss of exon 16
lower sequence: GRMZM2G036050_T03 (Zea mays), 3'ss of exon 17
-------------------------aacttggaagcagatgttaaccaggctgtaagcatgagtaattatagctgttgatacattctgaaaccattgaaat--tcagggctaagagatttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGCAAGATTGGTGATGCTGGCCAAAAG
                          | ||       |||  ||   ||   | | | || | ||||  |   ||||| |  || ||||||||||||| ||  ||       | |||  | ||||||||||||||||| || ||||||||||||||||| ||||||||||   |||||||  |||||||| ||  || ||||
ttggttgatgaaaggtgcagcatatgatttttggttggataatattaagcaggcagggataaataatctt---tgttggtgtatactgaaaccattgacatagTCGCTAAGGAAAGAGCTGTTGCAGGTTGAATTTGCCGGAGGTGCTTTACGGTGTGGCGAATACATCATGGTGCGCAAAGTTGGTGATTCTATCCTAAAG

upper sequence: LOC_Os09g39590.1 (Oryza sativa), 3'ss of exon 16
lower sequence: GRMZM2G036050_T04 (Zea mays), 3'ss of exon 16
-aacttggaagcagatgttaaccaggctgtaagcatgagtaattatagctgttgatacattctgaaaccattgaaat--tcagggctaagagatttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGCAAGATTGGTGATGCTGGCCAAAAG
  | ||       |||  ||   ||   | | | || | ||||  |   ||||| |  || ||||||||||||| ||  ||       | |||  | ||||||||||||||||| || ||||||||||||||||| ||||||||||   |||||||  |||||||| ||  || ||||
tgatttttggttggataatattaagcaggcagggataaataatctt---tgttggtgtatactgaaaccattgacatagtcgctaaggaaagagctgttgcagGTTGAATTTGCCGGAGGTGCTTTACGGTGTGGCGAATACATCATGGTGCGCAAAGTTGGTGATTCTATCCTAAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|88541812|gb|CI471105.1|CI471105
EST:     TTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAG                         GTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
genomic: TTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAGgtaagttttc ... tttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
EST: gi|88829475|gb|CI074345.1|CI074345
EST:     ATTTGAAATTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAG                         GTTGAATTTGCTGGTGGTGCTATACGGTGTGGTGAATACATCACACTGCGC
genomic: ATTTGAAATTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAGgtaagttttc ... tttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
EST: gi|88527394|gb|CI455552.1|CI455552
EST:     ATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAG                         GTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
genomic: ATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAGgtaagttttc ... tttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
EST: gi|88535348|gb|CI464143.1|CI464143
EST:     CAATTCCTGGCAAATAAAGGTTTGCAG                         GTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
genomic: CAATTCCTGGCAAATAAAGGTTTGCAGgtaagttttc ... tttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
EST: gi|88531680|gb|CI460090.1|CI460090
EST:     TTTGAAATTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAG                         GTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
genomic: TTTGAAATTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAGgtaagttttc ... tttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
EST: gi|88964230|gb|CI045423.1|CI045423
EST:     ATTTGAAATTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAG                         GTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
genomic: ATTTGAAATTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAGgtaagttttc ... tttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
EST: gi|29672566|gb|CB668841.1|CB668841
EST:     ATTTGAAATTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAG                         GTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
genomic: ATTTGAAATTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAGgtaagttttc ... tttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
EST: gi|88560284|gb|CI490933.1|CI490933
EST:     CAATTCCTGGCAAATAAAGGTTTGCAG                         GTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC
genomic: CAATTCCTGGCAAATAAAGGTTTGCAGgtaagttttc ... tttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 74 (upstream exon), 49 (downstream exon)
Score: 11

GTTGCCCCCGTCGTCAACTCCAGCAGCCCACAAGTCAGTTCTTGTGGGTGATTTGAAATTGGCTGATTTTAAGCAATTCCTGGCAAATAAAGGTTTGCAGgtaagttttc ... tttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGCAAGATTGGTGATGCTGGCCAAAAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agctgttgatacattctgaaaccattgaaattcagggctaagagatttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGCAAGATTGGTGATGCTGGCCAAAAG
   tgttgat  putative branch site (score: 11)
                                             tttcttgc  putative PPT
                       attgaaatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
aacttggaagcagatgttaaccaggctgtaagcatgagtaattatagctgttgatacattctgaaaccattgaaattcagggctaagagatttcttgcagGTTGAATTTGCTGGTGGTGCTTTACGGTGTGGTGAATACATCACACTGCGCAAGATTGGTGATGCTGGCCAAAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  ATGCTG
- - - - -gcagatg
- - - - - - - - - - -caggctg
- - - - - - - - - - - - - - -aagcatg
- - - - - - - - - - - - - - - - - - - - - - - gctgttg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - cattctg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -aaaccat