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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaaacgcgcgcaccacgccgaccgatatgctgcacccttcgccgtcggctcgtctgatttgttgttctccttcttccatacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGCGACGTGTACAGCTTCGGGGTGGTGATGCTCGAGCTGATCATCGCCAAGC

Basic information

species Oryza sativa
transcript LOC_Os03g21230.1
intron # 16
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os03g21230.1 (Oryza sativa), 3'ss of exon 16
lower sequence: GRMZM2G023715_T02 (Zea mays), 3'ss of exon 18
----------------------------gtaaacgcgcgcaccac----gccgaccgatatgctgcacccttcg-----ccgtcggctcgtctgatttg---ttgttctccttcttcc--atacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGCGACGTGTACAGCTTCGGGGTGGTGATGCTCGAGCTGATCATCGCCAAGC
                               | |||   |||  |    | |  |||||  |||||| ||  ||         ||  ||   |||| ||   |||   | |||| |||  | || |||||||||| ||||| || ||||||||||||||||||||||||||||||||||||||||| ||||||||||| ||||| ||||| ||||||||| |||| ||||
gtacgccgccgactagttttcatcgatcagcagcgctgtcactgcatccgtcatccgatccgctgcagccaacgaacgattcccgcttccggtgatctgaccttgcgttgcttcatccggaaacgtgcagGGCTATCTGGACCCTGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGCGACGTCTACAGCTTCGGCGTGGTCATGCTGGAGCTGATCGTCGCGAAGC

upper sequence: LOC_Os03g21230.1 (Oryza sativa), 3'ss of exon 16
lower sequence: GLYMA16G18090.1 (Glycine max), 3'ss of exon 18
----------gtaaacgcgcgc--accacgccgaccgatatgctgcacccttcgccgtcggctcgtctgatttgttgttctccttcttccatacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGCGACGTGTACAGCTTCGGGGTGGTGATGCTCGAGCTGATCATCGCCAAGC
          |||||  | |         | |   |  | || |    | ||     | |||        |||| |  |     |  |  | |  ||||||| || |||||||||||||| |||||| | || || || || |||||||| || |||||||| || ||||| || ||||| || || || |   | |  |
atcactaattgtaaaatctccatggttgtgtctttcattttgatttcacttttatttttggcaacaactgtttgataattgaaatgcttaacatctgcagGGTTATCTGGATCCCGAGTATTACATGACGCAACAACTCACAGAGAAGAGTGATGTGTACAGTTTTGGGGTAGTCATGCTTGAACTAATAACTTCAAGAC
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|87039254|gb|CI193876.1|CI193876
EST:     CAAAGGAACACTG                         GGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
genomic: CAAAGGAACACTGgtaaacgcgc ... atacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
EST: gi|87022820|gb|CI269756.1|CI269756
EST:     TGTCCGACACCGAGAGGGGCCACGTCAGCACCCAAGTCAAAGGAACACTG                         GGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
genomic: TGTCCGACACCGAGAGGGGCCACGTCAGCACCCAAGTCAAAGGAACACTGgtaaacgcgc ... atacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
EST: gi|87038372|gb|CI192994.1|CI192994
EST:     CAAAGGAACACTG                         GGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
genomic: CAAAGGAACACTGgtaaacgcgc ... atacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
EST: gi|86517985|gb|CI158191.1|CI158191
EST:     AAGGAACACTG                         GGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
genomic: AAGGAACACTGgtaaacgcgc ... atacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
EST: gi|87040179|gb|CI194801.1|CI194801
EST:     AAAGGAACACTG                         GGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
genomic: AAAGGAACACTGgtaaacgcgc ... atacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
EST: gi|88198695|gb|CI198545.1|CI198545
EST:     CAAAGGAACACTG                         GGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
genomic: CAAAGGAACACTGgtaaacgcgc ... atacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
EST: gi|29660306|gb|CB656581.1|CB656581
EST:     TGTCCGACACCGAGAGGGGCCACGTCAGCACCCAAGTCAAAGGAACACTG                         GGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC
genomic: TGTCCGACACCGAGAGGGGCCACGTCAGCACCCAAGTCAAAGGAACACTGgtaaacgcgc ... atacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 66 (upstream exon), 67 (downstream exon)
Score: 11

GGACGAGCACCTCACGGCCAAGGTCGCCGACTTCGGCCTCTCCAAGCTAGTGTCCGACACCGAGAGGGGCCACGTCAGCACCCAAGTCAAAGGAACACTGgtaaacgcgc ... atacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGCGACGTGTACAGCTTCGGGGTGGTGATGCTCGAGCTGATCATCGCCAAGC




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cacccttcgccgtcggctcgtctgatttgttgttctccttcttccatacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGCGACGTGTACAGCTTCGGGGTGGTGATGCTCGAGCTGATCATCGCCAAGC
                   gtctgat  putative branch site (score: 11)
                         tttgttgttctccttc  CT-rich tract
                        atttgtt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaaacgcgcgcaccacgccgaccgatatgctgcacccttcgccgtcggctcgtctgatttgttgttctccttcttccatacttgcagGGCTACCTGGATCCCGAGTACTACATGTCCCAGCAGCTGACCGAGAAGAGCGACGTGTACAGCTTCGGGGTGGTGATGCTCGAGCTGATCATCGCCAAGC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGAT