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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttgttagatgcactcttcagttgcaattttggtgtgtttggatcaagtttgatacacgattttgttaatttggagttcttttttcctttttgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGTGGAACATTGGGTGGTATTGTTACAACAGTTGAAGGTTTAATTGTGAAAA

Basic information

species Oryza sativa
transcript LOC_Os08g36774.1
intron # 12
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os08g36774.1 (Oryza sativa), 3'ss of exon 12
lower sequence: GRMZM2G351582_T01 (Zea mays), 3'ss of exon 13
-----ttgttagatgcactcttcagttgcaattttggtgtgtttggatcaagtttgatacacgattttgttaatttggagttcttttttcctttttgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGTGGAACATTGGGTGGTATTGTTACAACAGTTGAAGGTTTAATTGTGAAAA
      | || || ||||       |    | |   |    | |    || |    ||   | | | |   | ||||      || |||   ||||||||| ||||||||||||||| ||||||||||||||||||| |||||||| ||  || ||||||| ||||||||||| || ||||| ||||||||||||||||||||||
tgattctattggaggcacatacttatatagactaaagaacttgttagacaggacccatcttctaatatacaactttg-----tttatttgggttttgtttttcagTCAGATTCAGCTAGCGTAAAAGTACCTGAACTTGAGTTGGAACTTTCATGTGGAACGTTGGGTGGTATGGTCACAACTGTTGAAGGTTTAATTGTGAAAA

upper sequence: LOC_Os08g36774.1 (Oryza sativa), 3'ss of exon 12
lower sequence: GLYMA07G34890.3 (Glycine max), 3'ss of exon 6
ttgttagatgcactcttcagttgcaattttggtgtgtttggatcaagtttgatacacgattttgttaatttggagttcttttttcctt--tttgtttt--ccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGTGGAACATTGGGTGGTATTGTTACAACAGTTGAAGGTTTAATTGTGAAAA
 || | ||| | |   |  |    |   ||    | |||    ||| | ||| |||      ||| | ||   |  | ||   |   |  |||| |||    |||| ||| | ||  | |||||||| |||||||| || |||||||| ||||| |||||  | || || |||||||| || |||||||||||||||   ||||
-tgat-gatacccatataggcctgatcatttaaattttttctgcaa-tctgagacatagaaatgt-atttcatatctattgactgagtagtttggtttgattagTCTGATACTGCAAGTGTAAAAGTTCCTGAACTTGACTTGGAGCTGGCAAGCGGAACCCTTGGAGGAATTGTTACTACTGTTGAAGGTTTAATTACAAAAA

upper sequence: LOC_Os08g36774.1 (Oryza sativa), 3'ss of exon 12
lower sequence: GLYMA20G02690.1 (Glycine max), 3'ss of exon 12
ttgttagatgcactcttcagttgcaattttggtgtgtttggatcaagtttgatacacgattttg---ttaatttggagttcttttttcctttttgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGTGGAACATTGGGTGGTATTGTTACAACAGTTGAAGGTTTAATTGTGAAAA
 || | ||| | |   |  |    |   || |  |||||    ||| | ||| ||     | ||   || || |  | |   |   |  |||   ||    |||| ||| | ||  | |||||||| |||||||| || |||||||| ||||| |||||  | || || ||||||||||| |||||||||||||||   ||||
-tgat-gatacccatataggcctgaccatttgaatgttttctgcaa-tctgagacgtagatatgcatttcatatcaattgactgagtagtttggtttgattagTCTGATACTGCAAGTGTAAAAGTTCCTGAACTTGACTTGGAGCTGGCAAGCGGAACCCTTGGAGGAATTGTTACAACTGTTGAAGGTTTAATTACAAAAA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|29642058|gb|CB647065.1|CB647065
EST:     TTACACTACGTGTGCAGAACGGAAAAGATCTTACACGTGATGTCATAAAG                         TCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGT
genomic: TTACACTACGTGTGCAGAACGGAAAAGATCTTACACGTGATGTCATAAAGgtcagaaatt ... tgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGT
EST: gi|88225974|gb|CI225890.1|CI225890
EST:     TTACACTACGTGTGCAGAACGGAAAAGATCTTACACGTGATGTCATAAAG                         TCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGT
genomic: TTACACTACGTGTGCAGAACGGAAAAGATCTTACACGTGATGTCATAAAGgtcagaaatt ... tgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGT
EST: gi|88219195|gb|CI220468.1|CI220468
EST:     GAAAAGATCTTACACGTGATGTCATAAAG                         TCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGT
genomic: GAAAAGATCTTACACGTGATGTCATAAAGgtcagaaatt ... tgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGT
EST: gi|218492385|gb|FG960643.1|FG960643
EST:     TTACACTACGTGTGCAGAACGGAAAAGATCT-ACACGTGATGTCATAAAG                         TCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGT
genomic: TTACACTACGTGTGCAGAACGGAAAAGATCTTACACGTGATGTCATAAAGgtcagaaatt ... tgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGT
EST: gi|29633610|gb|CB638619.1|CB638619
EST:     TTACACTACGTGTGCAGAACGGAAAAGATCTTACACGTGATGTCATAAAG                         TCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGT
genomic: TTACACTACGTGTGCAGAACGGAAAAGATCTTACACGTGATGTCATAAAGgtcagaaatt ... tgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 45 (upstream exon), 41 (downstream exon)
Score: 9

TTGAAGCCTGGTGGTGAAATCCCAGCCAAAGGAAAGAAAATTACACTACGTGTGCAGAACGGAAAAGATCTTACACGTGATGTCATAAAGgtcagaaatt ... tgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGTGGAACATTGGGTGGTATTGTTACAACAGTTGAAGGTTTAATTGTGAAAA




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agtttgatacacgattttgttaatttggagttcttttttcctttttgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGTGGAACATTGGGTGGTATTGTTACAACAGTTGAAGGTTTAATTGTGAAAA
                 tgttaat  putative branch site (score: 9)
                              ttcttttttccttttt  putative PPT
             attttgttaattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttgttagatgcactcttcagttgcaattttggtgtgtttggatcaagtttgatacacgattttgttaatttggagttcttttttcctttttgttttccagTCAGATTCAGCTGGCGTAAAAGTACCTGAACTCGAGTTGGAGCTAGCAAGTGGAACATTGGGTGGTATTGTTACAACAGTTGAAGGTTTAATTGTGAAAA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TTGAAG
- - - gatgcac
- - - - - - - - -cagttgc
- - - - - - - - - - - - - - - - -gtgtttg
- - - - - - - - - - - - - - - - - - - - - - - - - gatacac