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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ctctagcttatacctggaaggttttgccgaattcacctattttcatgtttgatcttctcttgtacatggagagtcactttctgtatcttatattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGATGTTCTATCTGTTCTAATGGAGCAGCATGAGCTATTTCTAAGCTCAACTC

Basic information

species Oryza sativa
transcript LOC_Os04g58130.1
intron # 12
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os04g58130.1 (Oryza sativa), 3'ss of exon 12
lower sequence: GRMZM2G160702_T01 (Zea mays), 3'ss of exon 11
ctctagcttatacctggaaggttttgccgaattcacctattttcatgtttgatcttct----cttgtacatggagagtcactttctgtatcttatattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGATGTTCTATCTGTTCTAATGGAGCAGCATGAGCTATTTCTAAGCTCAACTC
  || |  | ||  ||  |           || ||   | || ||||||||  | |||     || ||||  || |   |       | ||||   || ||||||||||||||| ||||||| ||||| ||||||  ||||| |||||||| |||||||||||| || | |||||||  ||||| |||||| ||||||||||| 
--cttgactttatttg--acacaacagtttatacatggaattgcatgtttggccatctatgctttttacacagatatatatgaaacctttcttcccttctgcagAGAGGAAGCAATCAAGCTTCTGAAGCTCCAATCATAGCTAGTGATGAAGATGTTCTATCTCTTATCATGGAGCGACATGATCTATTTTTAAGCTCAACTA

upper sequence: LOC_Os04g58130.1 (Oryza sativa), 3'ss of exon 12
lower sequence: GRMZM2G406553_T02 (Zea mays), 3'ss of exon 12
ctctagcttatacctggaaggttttgccgaattcacctattttcatgtttgatcttctct--tgtacatggagagtcactttctgtatcttatattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGATGTTCTATCTGTTCTAATGGAGCAGCATGAGCTATTTCTAAGCTCAACTC
   ||   | ||  |  ||    |      || |    | || ||| ||||  | ||| |  | |  |   |||   || |      | |  | || ||||||||||||||||||||||| ||||||||||||  ||||| | |||||| |||||| ||||| || | ||||| || |||||| ||||||||||||| ||  
tcttaaactttattt--aacacatcagtttatacctggaattgcatatttggccatctatgctttttacacagatctacatgaaacctttcctcttctgcagAGAGGAAGCAGTCAAGCTTCTGAAACTCCAATCATAGCTAATGATGAAGATGTTATATCTCTTTTCATGGAACAACATGAGTTATTTCTAAGCTCGACAA
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|58684637|gb|CK073324.1|CK073324
EST:     CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCT                         AGAGGAAGCAGTCCAAGCTGCTG
genomic: CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCTgtatgtgtga ... tattttgcagAGAGGAAGCAGTC-AAGCTGCTG
EST: gi|112786515|gb|EE590909.1|EE590909
EST:     CAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCT                         AGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGAT
genomic: CAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCTgtatgtgtga ... tattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGAT
EST: gi|29644693|gb|CB649700.1|CB649700
EST:     CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCT                         AGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGAT
genomic: CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCTgtatgtgtga ... tattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGAT
EST: gi|58670430|gb|CK059116.1|CK059116
EST:     CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCT                         AGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATATCAAGTGATGAGGAT
genomic: CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCTgtatgtgtga ... tattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGAT
EST: gi|29660533|gb|CB656808.1|CB656808
EST:     CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCT                         AGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGAT
genomic: CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCTgtatgtgtga ... tattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGAT
EST: gi|33796149|gb|CF323940.1|CF323940
EST:     CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCT                         AGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGAT
genomic: CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCTgtatgtgtga ... tattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGAT
EST: gi|58694610|gb|CK083297.1|CK083297
EST:     CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCT                         AGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATATCAAGTGATGAGGAT
genomic: CAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCTgtatgtgtga ... tattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 57 (upstream exon), 66 (downstream exon)
Score: 23

TGCTTCTAATTGGGAAAGCAGAGATCAGTCACCTGGTAATGAAGAACCGACAACAAGCAATTCTTCTTCGATGGCTCCTACAGGCCGATTATATTCATCTgtatgtgtga ... tattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGATGTTCTATCTGTTCTAATGGAGCAGCATGAGCTATTTCTAAGCTCAACTC




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgtttgatcttctcttgtacatggagagtcactttctgtatcttatattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGATGTTCTATCTGTTCTAATGGAGCAGCATGAGCTATTTCTAAGCTCAACTC
 gtttgat  putative branch site (score: 23)
                                    tgtatcttatatttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ctctagcttatacctggaaggttttgccgaattcacctattttcatgtttgatcttctcttgtacatggagagtcactttctgtatcttatattttgcagAGAGGAAGCAGTCAAGCTGCTGAAACTCCAACGATAGCAAGTGATGAGGATGTTCTATCTGTTCTAATGGAGCAGCATGAGCTATTTCTAAGCTCAACTC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTCA
- - - - - tacctgg
- - - - - - - - - - - tttgccg
- - - - - - - - - - - - - - - - - - - - tttcatg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gtacatg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ctgtatc