ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtaactatggtatttcgtttatgatggtatttatgtaggattattcccaaattattcttacctgagttttcctgctgtacataagGGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAGATGAGCTGGACCGAACGAAATATCCTCGCATGCCTTGGCATGATGTTCAGTGTGCTCTCTAC

Basic information

species	Oryza sativa
transcript	LOC_Os05g29050.2
intron #	11
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os05g29050.2 (Oryza sativa), 3'ss of exon 11
lower sequence: GRMZM2G066485_T01 (Zea mays), 3'ss of exon 9

gtaactatggtatttcgtttatga-tggtatttatgtaggattattcc-caaattattcttacctgagttttcctgctgtacataagGGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAGATGAGCTGGACCGAACGAAATATCCTCGCATGCCTTGGCATGATGTTCAGTGTGCTCTCTAC
         |||||| |||  |   |  ||   ||  ||   |||    |||| |      |  | | ||   || ||||| ||| |||||||||| || ||||||||||||||||| ||||||||||||||||||||    |||||||| ||||||||||||||||||||||||||||||||||| 
---------gtattttgttgttatcttctacccattcagatgtatggggcaaagtccctaaatgtcacttcatct-ctgtaaata-gGGAATCTGAACCCAATTCCTGGGAGGACACCATGAAAGATGAGCTGGACCGTGATAAATATCCCCGCATGCCTTGGCATGATGTTCAGTGTGCTCTCTAT

upper sequence: LOC_Os05g29050.2 (Oryza sativa), 3'ss of exon 11
lower sequence: GLYMA09G04620.1 (Glycine max), 3'ss of exon 10

----------gtaactatggtatttcgtttatgatggtatttatgtaggattattcccaaattattct-------tacctgagttttcctgctgtacataagGGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAGATGAGCTGGACCGAACGAAATATCCTCGCATGCCTTGGCATGATGTTCAGTGTGCTCTCTAC
          ||   || | ||| || || || ||   ||| ||    || ||   |     | |||        ||   || |  |  |    |     || |||||||| |||||||| ||||| || ||||||||||||||  | || ||    ||||||||||| |||||||||||||||||||| ||||| || |  
cactttgaatgtg-ttaagatatgtcattcattatta-attgatcatagactagctctttcctgttcaacgatagtaatagattcattttcaattttgacagAGAATCTGAACCAAATTCATGGGAAGATACAATGAAAGATGAATTAGAACGTGAAAAATATCCTCGTATGCCTTGGCATGATGTTCATTGTGCCCTTTGG

upper sequence: LOC_Os05g29050.2 (Oryza sativa), 3'ss of exon 11
lower sequence: GLYMA15G16270.1 (Glycine max), 3'ss of exon 10

-------gtaactatggta---tttcgtttatgatgg--tatttatgtaggattattcccaaattattcttacctgagttttcctgctgtacata---agGGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAGATGAGCTGGACCGAACGAAATATCCTCGCATGCCTTGGCATGATGTTCAGTGTGCTCTCTAC
        | | | || ||   | || || || ||    || | ||  |    | || ||   | |   |||      | ||| |  |  |  |    || |||||||| || ||||| ||||| || ||||||||||||||  | || ||    ||||||||||| |||||||||||||||||||| ||||| || |  
ttgcactttgaatgtgttaagatgtcattcatcattaattaatcatagacagctctttcctgctgaacgttagtaatatattcattttcaattttgacagAGAATCTGAACCTAATTCATGGGAAGATACAATGAAAGATGAATTAGAACGTGAAAAATATCCTCGTATGCCTTGGCATGATGTTCATTGTGCCCTTTGG

upper sequence: LOC_Os05g29050.2 (Oryza sativa), 3'ss of exon 11
lower sequence: GLYMA20G38200.1 (Glycine max), 3'ss of exon 11

--------gtaactatggta----tttcgtttatgatggtatttatgtaggattattcccaaattattcttacctgagtttt---cctgctgtacataagGGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAGATGAGCTGGACCGAACGAAATATCCTCGCATGCCTTGGCATGATGTTCAGTGTGCTCTCTAC
         || |  || |      |||  |  || || |   | ||      ||    |  | || | |||  | | || |     || ||      || ||||||||||||||||| ||||| ||||| |||||||||||  |||| || | ||| ||||| || ||||| ||||| ||||| || |||||||| |  
cattacaattagccgtgttggagccttcactattgctgatgagtctgcttttctaagttcttagtaatattatataatttgtaattttgttggttgatagAGAATCTGAGCCAAATTCATGGGAAGACACCATGAAAGATGAATTGGATCGCAAGAAGTATCCCCGTATGCCATGGCACGATGTCCATTGTGCTCTTTGG

upper sequence: LOC_Os05g29050.2 (Oryza sativa), 3'ss of exon 11
lower sequence: Vv05s0077g01830.t01 (Vitis vinifera), 3'ss of exon 11

-----------gtaactatggtatttcgtttatgatggtatt-tatgtaggattattcccaaattattc----ttacctgagttttcctg----ctgtacataagGGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAGATGAGCTGGACCGAACGAAATATCCTCGCATGCCTTGGCATGATGTTCAGTGTGCTCTCTAC
            | || || || | |  |||   | |  |||  | ||  | ||   | |    | ||     ||   | | || |  ||     |  |  | ||||||||||| |||||||| ||||| || ||||||||||||||  |||| ||    ||||||||||| ||||| |||||||| || || ||||| || |  
gtgtgagaacattgacaatagtttcttcttttctaagtaattgcaagtttgttttcactctggctctttcacattggataacttgtaatgaattttcaatttcagGGAATCTGAACCAAATTCTTGGGAAGATACAATGAAAGATGAATTGGATCGTGGAAAATATCCTCGTATGCCATGGCATGACGTCCATTGTGCCCTATGG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|58669537|gb|CK058223.1|CK058223

EST:     TCAGATGTTCCTCCTGTGATATGGCCA-GGAAAGACTACTATAACCCCAG                         GGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAGATGAGCTGGACCG
genomic: TCAGATGTTCCTCCTGTGATATGGCCAGGGAAAGACTACTATAACCCCAGgtaactatgg ... tgtacataagGGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAGATGAGCTGGACCG

EST: gi|58692366|gb|CK081053.1|CK081053

EST:     TCAGATGTTCCTCCTGTGATATGGCCAGGGAAAGACTACTATAACCCCAG                         GGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAG
genomic: TCAGATGTTCCTCCTGTGATATGGCCAGGGAAAGACTACTATAACCCCAGgtaactatgg ... tgtacataagGGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 66 (upstream exon), 27 (downstream exon)
Score: 5

GAGGTCTTGATCTGTGCTTTGGTCGCTACGATAATTCTGCGCACAAACTTTCAGATGTTCCTCCTGTGATATGGCCAGGGAAAGACTACTATAACCCCAGgtaactatgg ... tgtacataagGGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAGATGAGCTGGACCGAACGAAATATCCTCGCATGCCTTGGCATGATGTTCAGTGTGCTCTCTAC

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atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttatgtaggattattcccaaattattcttacctgagttttcctgctgtacataagGGAATCTGAGCCAAATTCCTGGGAGGACACAATGAAAGATGAGCTGGACCGAACGAAATATCCTCGCATGCCTTGGCATGATGTTCAGTGTGCTCTCTAC