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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tctgtgccagcttggtcttgatagcattgctatcagtaactacttcatcgagtactttcatgttgcataaattttatcctgaattcgtaatgctttgcagGGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT

Basic information

species Oryza sativa
transcript LOC_Os12g40830.3
intron # 10
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os12g40830.3 (Oryza sativa), 3'ss of exon 10
lower sequence: GRMZM2G069758_T01 (Zea mays), 3'ss of exon 10
tctgtgc-cagcttggtcttgatag--cattgctatcagtaactacttcatcgagtactttcatgttgcataaattttatcctgaattcgtaatgctttgcagGGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT
|| | ||| | |||| |||| || ||| |||| | | || | | | | | |||||||| |||| | | ||||||| ||||||| ||||||||||| |||||||| || ||||||||||||||||||
--tgcccacagtaggttcttaatagattataattattagtatatgtataattcaat-cacctgtataaattaaattttcacctggctccataatgctatgcagGGAACTGGAGATTTGACAACTGCTCTCCTACTAGGATGGAGTAAT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|568331|gb|D39176.1|D39176
EST:     CACCAGAACAATTTAAGATTGAGATACCCAAGATACCTGCATATTTCACG                         GGGCACTGGAGATTTAACAACTG
genomic: CACCAGAACAATTTAAGATTGAGATACCCAAGATACCTGCATATTTCACGgtatgcactc ... tgctttgcagGG-CACTGGAGATTTAACAACTG
EST: gi|427643|gb|D23776.1|D23776
EST:     CACCAGAACAATTTAAGATTGNGATACCCAAGATACCTGCATATTTCACG                         GGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGNGTAAT
genomic: CACCAGAACAATTTAAGATTGAGATACCCAAGATACCTGCATATTTCACGgtatgcactc ... tgctttgcagGGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT
EST: gi|86885325|gb|CI317085.1|CI317085
EST:     ATACCTGCATATTTCACG                         GGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT
genomic: ATACCTGCATATTTCACGgtatgcactc ... tgctttgcagGGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT
EST: gi|13165352|gb|AU174149.1|AU174149
EST:     CACCAGAACAATTTAAGATTGAGATACCCAAGATACCTGCATATTTCACG                         GGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT
genomic: CACCAGAACAATTTAAGATTGAGATACCCAAGATACCTGCATATTTCACGgtatgcactc ... tgctttgcagGGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT
EST: gi|89185094|gb|CI049640.1|CI049640
EST:     CACCAGAACAATTTAAGATTGAGATACCCAAGATACCTGCATATTTCACG                         GGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT
genomic: CACCAGAACAATTTAAGATTGAGATACCCAAGATACCTGCATATTTCACGgtatgcactc ... tgctttgcagGGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT
EST: gi|88543303|gb|CI473300.1|CI473300
EST:     TTAAGATTGAGATACCCAAGATACCTGCATATTTCACG                         GGCACTGGAGATTTAACAACTGCCCTTTTACTAGGATGGAGTAAT
genomic: TTAAGATTGAGATACCCAAGATACCTGCATATTTCACGgtatgcactc ... tgctttgcagGGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 57 (upstream exon), 45 (downstream exon)
Score: 30

GAACAACCACCAGAACAATTTAAGATTGAGATACCCAAGATACCTGCATATTTCACGgtatgcactc ... tgctttgcagGGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

catcgagtactttcatgttgcataaattttatcctgaattcgtaatgctttgcagGGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT
                 ttgcataaattttat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tctgtgccagcttggtcttgatagcattgctatcagtaactacttcatcgagtactttcatgttgcataaattttatcctgaattcgtaatgctttgcagGGCACTGGAGATTTAACAACTGCCCTTCTACTAGGATGGAGTAAT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GATGGA
- -gtgccag
- - - - - - - - - - - -gcattgc
- - - - - - - - - - - - - - - - - - - - - cttcatc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - -atgttgc