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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tgttctgatttcattaccttaattgaacctaccaactaagtctggatttaaatatttttggcgctggtgctgataatggccttgtgccactgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAATGCACTTAAGAACAACTCTGATTCAAGATAAACAG

Basic information

species Oryza sativa
transcript LOC_Os05g08980.4
intron # 10
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os05g08980.4 (Oryza sativa), 3'ss of exon 10
lower sequence: GRMZM2G558539_T01 (Zea mays), 3'ss of exon 11
------tgttctgatttcattaccttaattgaacctaccaactaagtctggatttaaatatttttggcgctggtgctgataatggccttgtgccactgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAATGCACTTAAGAACAACTCTGATTC-------AAGATAAACAG
      | |   | | |  ||    | ||  ||| || |||  ||      |||| | ||   |    ||  ||  | | | |||    |    | | | |||||||| ||||||||||| |||||| ||||||||| || |||||| ||  |  ||   ||   |||  |  |||||||       || ||||||||
atcttatatctagttattcttgaactgatgaaacttaacaataaatatgccatttgactagcct----gcaagttgttacagtggaa--gctaaatttcatttcagGGATTGCAACTGGCAATAGAATCAGGGAATTGTGGCAAGGACACCATTGATATGCTGTTGAAAGATGCTGATTCCTCTGATAAAATAAACAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|86864610|gb|CI344188.1|CI344188
EST:     GGCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: GGCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|86420361|gb|CI056350.1|CI056350
EST:     GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|218505037|gb|FG952955.1|FG952955
EST:     AAGGCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: ----CTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|86414853|gb|CI069256.1|CI069256
EST:     GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|29663313|gb|CB659588.1|CB659588
EST:     AAGGCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: ----CTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|87032114|gb|CI362194.1|CI362194
EST:     GGCTCAAAAATGGCT-GGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: GGCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|29674385|gb|CB670660.1|CB670660
EST:     GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|88962073|gb|CI097226.1|CI097226
EST:     GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|86488427|gb|CI131060.1|CI131060
EST:     GGCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: GGCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|33684852|gb|CF313091.1|CF313091
EST:     GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|29671281|gb|CB667556.1|CB667556
EST:     AAGGCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: ----CTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|88417751|gb|CI535910.1|CI535910
EST:     ATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: ATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|29688958|gb|CB685233.1|CB685233
EST:     GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|33684851|gb|CF313090.1|CF313090
EST:     AAGGCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: ----CTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
EST: gi|88993942|gb|CI114232.1|CI114232
EST:     CTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAG                         GGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT
genomic: CTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 51 (upstream exon), 69 (downstream exon)
Score: 32

GCTCAAAAATGGCTGGGAAGCGTTTTGGAAGCCAAAATCTCATACAGgttagtagta ... tgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAATGCACTTAAGAACAACTCTGATTCAAGATAAACAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atttaaatatttttggcgctggtgctgataatggccttgtgccactgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAATGCACTTAAGAACAACTCTGATTCAAGATAAACAG
                      tgctgat  putative branch site (score: 32)
                                            ctgcttttc  putative PPT
     aatattttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgttctgatttcattaccttaattgaacctaccaactaagtctggatttaaatatttttggcgctggtgctgataatggccttgtgccactgcttttcagGGGTTGCAACTGGCCATAGAAACAGGGAATTTTGACAAGGAAACGTTCAATGCACTTAAGAACAACTCTGATTCAAGATAAACAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAC
tgttctg
- - - - tttcatt
- - - - - - - - - - - - - - - - caactaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ggcgctg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgctgat
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - cttgtgc