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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gatatgtgtaatcttgttagctaaatcataattttcgtatgtattcacatgacatttcgatgaatcaaactgatcgtagtacaattgtttctatagaaagATGGCGTCTCACATTGTTGGATACCCCCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCCCTGGAGTCTTTCTGGGATGGGAAGAGCAGCGCTGAGGATT

Basic information

species Oryza sativa
transcript LOC_Os12g42884.1
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os12g42884.1 (Oryza sativa), 3'ss of exon 1
lower sequence: GRMZM2G149751_T03 (Zea mays), 3'ss of exon 1
gatatgtgtaatcttgttagctaaatcat--aattttcgtatgtattcacatgacatttcgatgaatcaaactgatcgtagtacaattgtttctatagaaagATGGCGTCTCACATTGTTGGATACCCCCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCCCTGGAGTCTTTCTGGGATGGGAAGAGCAGCGCTGAGGATT
  |     ||||| | |       ||||   ||  |  ||||   ||   |  | | ||       |    ||||  ||| | | |||  |   | |||||||||||||| ||||||||||||||||| |||||||||||||||||||||||||||||||| || ||||||||||||||||||||||||| |||||||||| 
--tgaccttaatcatatcgttctcatcaataaacatctgtattccttgttacaatagttgacatggtatttctgactgtaattctatttatggcacagaaagATGGCGTCCCACATTGTTGGATACCCTCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCACTTGAGTCTTTCTGGGATGGGAAGAGCACCGCTGAGGATC

upper sequence: LOC_Os12g42884.1 (Oryza sativa), 3'ss of exon 1
lower sequence: GRMZM2G149751_T02 (Zea mays), 3'ss of exon 1
--gatatgtgtaatcttgttagctaaatcat--aattttcgtatgtattcacatgacatttcgatgaatcaaactgatcgtagtacaattgtttctatagaaagATGGCGTCTCACATTGTTGGATACCCCCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCCCTGGAGTCTTTCTGGGATGGGAAGAGCAGCGCTGAGGATT
   ||     ||||| | |       ||||   ||  |  ||||   ||   |  | | ||       |    ||||  ||| | | |||  |   | |||||||||||||| ||||||||||||||||| |||||||||||||||||||||||||||||||| || ||||||||||||||||||||||||| |||||||    
agcatgaccttaatcatatcgttctcatcaataaacatctgtattccttgttacaatagttgacatggtatttctgactgtaattctatttatggcacagAAAGATGGCGTCCCACATTGTTGGATACCCTCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCACTTGAGTCTTTCTGGGATGGGAAGAGCACCGCTGAG----

upper sequence: LOC_Os12g42884.1 (Oryza sativa), 3'ss of exon 1
lower sequence: PP1S33_110V6.1 (Physcomitrella patens), 3'ss of exon 1
--gatatgtgtaatcttgttagctaaatcataattttcgtatgtattcacatgacatttcgatgaatcaaactgatcgtagtacaattgtttctatag----aaagATGGCGTCTCACATTGTTGGATACCCCCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCCCTGGAGTCTTTCTGGGATGGGAAGAGCAGCGCTGAGGATT
  || | |  |   |    |       | ||   ||     ||     || | |  | |  |   |||| |    |    ||  | | ||||||  ||    || |||||| || ||| | |||||||||||||| |||||||||||||||||||||||||| || || |||||  | |||||||| |||| |   ||||      
cggacacgcttgtccagaattttcttgtgatgcattgatggtgcggatac-taaactatttactcatcagatatcttcctgtgtattcgtttctgcagcagAAACGATGGCTTCCCACGTGGTTGGATACCCCCGTATGGGCCCCAAGAGGGAGCTCAAGTTCGCTCTAGAGTCGATGTGGGATGGCAAGACCCAGGCTGC-----

upper sequence: LOC_Os12g42884.1 (Oryza sativa), 3'ss of exon 1
lower sequence: PP1S33_110V6.2 (Physcomitrella patens), 3'ss of exon 1
-----gatatgtgtaatcttgttagctaaatcataattttcgtatgtattcacatgacatttcgatgaatcaaactgatcgtagtacaattgtttctatag----aaagATGGCGTCTCACATTGTTGGATACCCCCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCCCTGGAGTCTTTCTGGGATGGGAAGAGCAGCGCTGAGGATT
     || | |  |   |    |       | ||   ||     ||     || | |  | |  |   |||| |    |    ||  | | ||||||  ||    || |||||| || ||| | |||||||||||||| |||||||||||||||||||||||||| || || |||||  | |||||||| |||| |   ||        
tgacggacacgcttgtccagaattttcttgtgatgcattgatggtgcggatac-taaactatttactcatcagatatcttcctgtgtattcgtttctgcagCAGAAACGATGGCTTCCCACGTGGTTGGATACCCCCGTATGGGCCCCAAGAGGGAGCTCAAGTTCGCTCTAGAGTCGATGTGGGATGGCAAGACCCAGGC--------

upper sequence: LOC_Os12g42884.1 (Oryza sativa), 3'ss of exon 1
lower sequence: PP1S27_81V6.1 (Physcomitrella patens), 3'ss of exon 1
----------gatatgtgta-atcttgttagctaaatcat-aattttcgtatgtattcacatgacatttcga--tgaatcaaactgatcgtagt--acaattgtttctatagaaag-
          | ||    |  ||||| ||  |   || || |   |  |    |||||| | ||  | ||    | |   ||| || ||   ||   ||   |||  ||   |||         ||||| || ||| | || ||||||||||| ||||||||||||||||||||||| || ||| |||||||| | ||||                        
acatggaaatggtagaattgcatcttattcacctgatgattagcgtgtgcgaatattcatacgatctctcaggctaacagaaattgttcactgtctgcagACGTTCTTAGTTAAATCATAAACCATGGCTTCCCACGTAGTCGGATACCCCCGGATGGGCCCCAAGAGGGAGCTCAAATTCGCCTTGGAGTCTATGTGGG------------------------

upper sequence: LOC_Os12g42884.1 (Oryza sativa), 3'ss of exon 1
lower sequence: PP1S399_19V6.3 (Physcomitrella patens), 3'ss of exon 1
-------gatatgtgtaatcttgtta---gctaaatcataattttcg-tatgtattcacatgacatttcgatgaatcaaactgatcgtagtacaattgtt--tctatagaaagATGGCGTCTCACATTGTTGGATACCCCCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCCCTGGAGTCTTTCTGGGATGGGAAGAGCAGCGCTGAGGATT
         | |  ||||     | |   | |    |||    |||| |||  |  |     | || |   |     ||  || | | |   |  |      | |  ||||  ||| | |||||| | |||||||| ||||||||||||||||||||||||||||||||||| || |||||  | |||||||| |||| |             
gtggtttagtgtacgtaactgaatgatgcgttggtgcatctcgttcggtatcgaaccgaggtatatcttcgttggctaatgtg-ttgcacctctctcacagATTTCAAGAACCATGACTTCTCACGTGGTTGGATATCCCCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCTCTTGAGTCGATGTGGGATGGCAAGACCC------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|88405893|gb|CI591288.1|CI591288
EST:     CTCCTCCCTCGCCGTCCGCCGCCGCCGCTCGCTTCCTCCCCTCTCCCACGAAAG                      
genomic: CTCCTCCCTCGCCGTCCGCCGCCGCCGCTCGCTTCCTCCCCTCTCCCAC----Ggtaagcccgc ... ctataga
EST: gi|58605690|gb|CK033723.1|CK033723
EST:     TTCCTCCCCTCTCCCACGAAAG                      
genomic: TTCCTCCCCTCTCCCAC----Ggtaagcccgc ... ctataga
EST: gi|58664144|gb|CK052830.1|CK052830
EST:     GCCGCCGCCGCTCGCTTCCTCCCCTCTCCCACGAAAG                      
genomic: GCCGCCGCCGCTCGCTTCCTCCCCTCTCCCAC----Ggtaagcccgc ... ctataga
EST: gi|569305|gb|D40154.1|D40154
EST:     CCTCCCTCGCCGTCCGCCGCCGCCGCTCGCTTCCTCCCCTCTCCCACGAAAG                      
genomic: CCTCCCTCGCCGTCCGCCGCCGCCGCTCGCTTCCTCCCCTCTCCCAC----Ggtaagcccgc ... ctataga
EST: gi|570088|gb|D40937.1|D40937
EST:     CGTCCGCCGCCGCCGCTCGCTTCCTCCCCTCTCCCACGAAAG                      
genomic: CGTCCGCCGCCGCCGCTCGCTTCCTCCCCTCTCCCAC----Ggtaagcccgc ... ctataga
EST: gi|300216097|gb|HO088723.1|HO088723
EST:     CTCCCTCGCCGTCCGCCGCCGCCGCTCGCTTCCTCCCCTCTCCCACGAAAG                      
genomic: CTCCCTCGCCGTCCGCCGCCGCCGCTCGCTTCCTCCCCTCTCCCAC----Ggtaagcccgc ... ctataga
EST: gi|58669089|gb|CK057775.1|CK057775
EST:     GCTCGCTTCCTCCCCTCTCCCACGAAAG                      
genomic: GCTCGCTTCCTCCCCTCTCCCAC----Ggtaagcccgc ... ctataga
EST: gi|29633667|gb|CB638676.1|CB638676
EST:     TCGCTTCCTCCCCTCTCCCACGAAAG                      
genomic: TCGCTTCCTCCCCTCTCCCAC----Ggtaagcccgc ... ctataga

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 30 (upstream exon), 75 (downstream exon)
Score: 3

GTCCTCCTCCTCCTCCTCCCTCGCCGTCCGCCGCCGCCGCTCGCTTCCTCCCCTCTCCCACGgtaagcccgc ... ctatagaaagATGGCGTCTCACATTGTTGGATACCCCCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCCCTGGAGTCTTTCTGGGATGGGAAGAGCAGCGCTGAGGATT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cacatgacatttcgatgaatcaaactgatcgtagtacaattgtttctatagaaagATGGCGTCTCACATTGTTGGATACCCCCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCCCTGGAGTCTTTCTGGGATGGGAAGAGCAGCGCTGAGGATT
                      aactgat  putative branch site (score: 3)
                                       ttgtttct  putative PPT
                                     aattgttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gatatgtgtaatcttgttagctaaatcataattttcgtatgtattcacatgacatttcgatgaatcaaactgatcgtagtacaattgtttctatagaaagATGGCGTCTCACATTGTTGGATACCCCCGCATGGGCCCCAAGAGGGAGCTCAAGTTTGCCCTGGAGTCTTTCTGGGATGGGAAGAGCAGCGCTGAGGATT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAGCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGGAT
- - - - - - - - - agctaaa
- - - - - - - - - - - - - - - - - - -tatgtat
- - - - - - - - - - - - - - - - - - - - - - -cacatga
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -atcaaac