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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tttcttgcatagagtgtagtctgtactagtactgttgagatcaaccatctgtagcaaagtagcaagcttgaattaatcaatggcgaaacaatggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACGTCGTCCAGGAACGCCAAGTGGTGGTACTCCGCCTTCCACAATGTCACCG

Basic information

species Oryza sativa
transcript LOC_Os08g03350.1
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os08g03350.1 (Oryza sativa), 3'ss of exon 1
lower sequence: GRMZM2G180659_T02 (Zea mays), 3'ss of exon 2
----------tttcttgcatagagtgtagtctgtactagtactgttgagatcaaccatctgtagcaaagtagcaagcttgaattaatcaatggcgaaacaatggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACGTCGTCCAGGAACGCCAAGTGGTGGTACTCCGCCTTCCACAATGTCACCG
            || ||||   | |  | | | ||    || |  || |  |||   | |||     |  ||| |      | |  |    ||| ||||||   | |||||| |   ||||||||| ||||||||| |||||||||||||| || ||||| |||||  ||||||||||||||||||||||||||||||||||||||||||
gagaccgcgacgtcctgca---attaaaatatatatgcttattcatgtg-ccaagactgtgtcagctaacagcgac-----aatggttgggggc-aaacaaaacttgcagGACGCGAGGAGCGCGCGGGAGAAGGCCATCGACGACTGGCTCCCGATCACCTCGTCGCGGAACGCCAAGTGGTGGTACTCCGCCTTCCACAATGTCACCG

upper sequence: LOC_Os08g03350.1 (Oryza sativa), 3'ss of exon 1
lower sequence: GRMZM2G046743_T01 (Zea mays), 3'ss of exon 1
----tttcttgcatagagtgtagtctgt----actagtact-gttgagatcaaccatctgtagcaaagtagcaagcttgaattaatcaatggcgaaacaatggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACGTCGTCCAGGAACGCCAAGTGGTGGTACTCCGCCTTCCACAATGTCACCG
    | |||||| | | |||| | |       |  || ||  |      ||| | | | |  ||||  | ||  |    ||  || |||   | |||      | ||||||||   ||||||| | ||||||||| |||||||| ||||| || ||||| |||||  ||||||||||||||||||||||||||||||||||||||||||
tctttctcttgctttgtgtgtgggcaaccaagatcagcacaagactgtgtcagctaacagcggcaa--tggcggg---aaaccaaacaaaaacaaaa----acttgcagGATGCGAGGAGCGCACGGGAGAAGGCCATCGACGAGTGGCTCCCGATCACCTCGTCGCGGAACGCCAAGTGGTGGTACTCCGCCTTCCACAATGTCACCG

upper sequence: LOC_Os08g03350.1 (Oryza sativa), 3'ss of exon 1
lower sequence: EFJ10950 (Selaginella moellendorffii), 3'ss of exon 1
--------------tttcttgca---tagagtgtagtct-gtactagtactgttgagatcaaccatctgtagcaaagtagcaagcttgaattaatcaatggcgaaacaatggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCAC--GTCG-TCCAGGAACGCCAAGTGGTGGTACTCCGCCTTCCACAATGTCACCG
               |||    |   | |     ||||  |   |    || ||     ||         |||    | |    || || | ||||  | |    |    |  | ||    ||||           | |||  |  | ||||| ||||| ||| |     || | |||||||||||||||||||||||||| |                  
gtaggaaagaatcccttcccaaaagctgggaaaaagtcccgagatccccctttttttcccagattctcacagctcc-tcgttttctggattgaatctgtagGAGTATGTCGAGGACAAA--GGCCACGCTCGAACTGTGAAATCCGTGGACGATTGGCTGCCCGTTGGCAGTGGCTCCAGGAACGCCAAGTGGTGGTACTCGG------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|29617404|gb|CB622416.1|CB622416
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|87005439|gb|CI285661.1|CI285661
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|13420701|gb|AU174791.1|AU174791
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|88347796|gb|CI655149.1|CI655149
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|88355012|gb|CI615893.1|CI615893
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|87004981|gb|CI308857.1|CI308857
EST:     ATCAGGTGATCATGGGGACT-AGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|88367905|gb|CI572626.1|CI572626
EST:     TCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCACAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCAC
genomic: TCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGC-CAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCAC
EST: gi|88334812|gb|CI647194.1|CI647194
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|29630537|gb|CB635546.1|CB635546
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|29616821|gb|CB621833.1|CB621833
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|29634583|gb|CB639592.1|CB639592
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|86826172|gb|CI311031.1|CI311031
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|29625305|gb|CB630316.1|CB630316
EST:     ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
EST: gi|44680081|gb|AB118076.1|AB118076
EST:     ACTACCCACCGGCCAAG                         GATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG
genomic: ACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 64 (upstream exon), 74 (downstream exon)
Score: 25

CTGATCCTCCATTGCTGCAAAAGAAGAAGAGGTAGACGAAGGAGGAGGAGATCAGGTGATCATGGGGACTCAGGTGGCAGATAACTACCCACCGGCCAAGgtatttgttt ... atggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACGTCGTCCAGGAACGCCAAGTGGTGGTACTCCGCCTTCCACAATGTCACCG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

catctgtagcaaagtagcaagcttgaattaatcaatggcgaaacaatggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACGTCGTCCAGGAACGCCAAGTGGTGGTACTCCGCCTTCCACAATGTCACCG
                         aattaat  putative branch site (score: 25)
                      ttgaattaatcaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tttcttgcatagagtgtagtctgtactagtactgttgagatcaaccatctgtagcaaagtagcaagcttgaattaatcaatggcgaaacaatggtggcagGATGGCCGGAGCGCGCAGGAGAAGGCGATCGACGACTGGCTTCCCATCACGTCGTCCAGGAACGCCAAGTGGTGGTACTCCGCCTTCCACAATGTCACCG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGTGG
- tcttgca
- - - - - - - - - - - - - - - -ctgttga
- - - - - - - - - - - - - - - - - - - - - - - - ctgtagc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - agcaagc