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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tttactgtattgcccccttttcatttctctcatttggattggttaaacgctatcaatatgtatttatcttgttaaaagaatctccttttctgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT

Basic information

species Oryza sativa
transcript LOC_Os05g30530.1
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os05g30530.1 (Oryza sativa), 3'ss of exon 1
lower sequence: PP1S461_1V6.1 (Physcomitrella patens), 3'ss of exon 1
tttactgtattgcccccttttcatttctctcatttggattggttaaacgctatcaatatgtatttatcttgttaaaagaatc--tccttttctgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT
  | |    ||             ||      |||| | | | |  | |  || |  | || |   || ||||   ||      | || || ||| |  |||||  | |||||||||||||||||||||||||||||||| ||||  |||||||||||||||||| | ||||| || || 
cctgcaagcttaaaatgggggtggtttagggttttgaagttggttgatg--atgaggaagtttgagtcgtgttctgagttctgtttctcttgtgcgttttagGCTCGTGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCGCCCAAGCATTGGATGCTTGACAAGTTGGGTGGTGCCTTC

upper sequence: LOC_Os05g30530.1 (Oryza sativa), 3'ss of exon 1
lower sequence: PP1S39_422V6.2 (Physcomitrella patens), 3'ss of exon 1
tttactgtattgcccccttttcatttctctcatttggattggttaaacgctatcaatatgtatttatc--ttgttaaaagaatctccttttctgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT
    |         |||| |  |  | | ||  || | |   ||  |   | |     |       ||     ||||         |     |  ||   ||||  ||||| | ||||||||| ||||| | || |||||||| || ||||||||||| |||||| | ||||| ||||| 
-aggcgaagacagaccctctggaggtgtgtcggtttg-tcaattggattttctttgcttccgaccttcggcacttaactatgatgtcacacttctttggcagGCTCGGGGTCTAAAGAAGCATTTGAAGCGACTGAATGCCCCTAGGCATTGGATGCTCGACAAGTTGGGTGGTGCATTC

upper sequence: LOC_Os05g30530.1 (Oryza sativa), 3'ss of exon 1
lower sequence: PP1S2_16V6.1 (Physcomitrella patens), 3'ss of exon 1
-----tttactgtattgcccccttttcatttctctcatttggattggttaaacgctatcaatatgtatttatcttgttaaaagaatctccttttctgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT
     |||||   ||||  | |||  ||||| | |  |  || ||    |   | | |     |||  |  |||  |  |   |   | |   |||   |   ||||  | ||||||||||||||| |||||||||||||||| ||||| || ||||||||||||||| | ||||| || |||
tttcgtttacgtcattgttcgctt--catttttgtggtc-ggtttatcgactggattttggcctgtcatggtctcctgtattcatctttccaatct--ctggcagGCTCGCGGTTTGAAGAAGCATTTGAAGAGGCTCAATGCGCCCAGGCACTGGATGCTTGACAAGTTGGGTGGTGCCTTT

upper sequence: LOC_Os05g30530.1 (Oryza sativa), 3'ss of exon 1
lower sequence: PP1S38_194V6.1 (Physcomitrella patens), 3'ss of exon 1
tttactgtattgcccccttttcatttctctcatttggattggttaaacgctatcaatatgtatttatcttgttaaaag--aatctccttttctgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT
 |||  |  || |      ||   ||  ||  |  | |  |       | |    |      | |  |||||||  |   |||    || |    |   |||||  | ||||||||||||||| |||||||||||||||| ||||| |||||||||||||||||| | ||||| || || 
-ttatcggtttacgtaaactt-gctttacttgtacgaagagtgcgtttgttcctgagtgacgtgtggcttgttacgaactaattgttttcttgtgtctctagGCTCGCGGTTTGAAGAAGCATTTGAAGAGGCTCAATGCTCCCAGACATTGGATGCTTGACAAGTTGGGTGGTGCCTTC

upper sequence: LOC_Os05g30530.1 (Oryza sativa), 3'ss of exon 1
lower sequence: PP1S81_172V6.1 (Physcomitrella patens), 3'ss of exon 1
tttactgtattgcccccttttcatttctctca--tttggattggttaaacgctatcaat-atgtatttatcttgttaaaagaatctccttttctgcttaat--agGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT
         |||    | |  ||| | | | |  | ||   || ||     || |   | || | ||||  |||||       | |   ||  |  ||  |  ||||  | ||||||||||||||| |||||||||||||||| ||||  ||||||||||| |||||  | ||||| || |||
-----gaggttggtttcgtagcatatttttgaaatgtgcgctgatttttttcttttttttatttttttacgttgtttttgttttttttgttatttttttcttcagGCTCGTGGTTTGAAGAAGCATTTGAAGAGGCTCAATGCGCCCAAGCATTGGATGCTCGACAAATTGGGTGGCGCCTTT

upper sequence: LOC_Os05g30530.1 (Oryza sativa), 3'ss of exon 1
lower sequence: PP1S282_25V6.1 (Physcomitrella patens), 3'ss of exon 1
tttactgtattgcccccttttcatttctctcatttgg-attggttaaacgctatcaatatgtatttatcttgttaaaagaatctccttttctgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT
  | ||| | ||            |||  |  ||||| | ||| | | | |  |    |  |  |     || ||||    | |     | |   |   ||||  | ||||||||||||||| |||||||||||||||| || |  |||||||||||||||||||| ||||| || || 
-atgctggagtgagaatcagggtcttcggtattttgggactggctgatcaccctgttcaattggtacgtgtgctaaacatgtgttgcggtgttgattccagGCTCGCGGTTTGAAGAAGCATTTGAAGAGGCTCAATGCGCCTAAGCATTGGATGCTTGACAAGCTGGGTGGCGCCTTC

upper sequence: LOC_Os05g30530.1 (Oryza sativa), 3'ss of exon 1
lower sequence: EFJ14824 (Selaginella moellendorffii), 3'ss of exon 1
tttactgtattgcccccttttcatttctctcatttggattggttaaacgctatcaatatgtatttatcttgttaaaagaatctccttttctgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT
                                                    | |    |      | | |    |     |   | || || |    ||| | | ||   |||||||||||| |||  ||||||||||||||  |||||||||||   |||||| || || || |||
---------------------------------------------------gtgagagcgcgcggctatcgcgggatcttacgattcttgtgttgtgcagGTGCGCGGGCCGAAGAAGCATCTCAAGGTGCTCAATGCCCCCAAGCATTGGATGCTGAGCAAGCTCGGCGGTGCCTTT

upper sequence: LOC_Os05g30530.1 (Oryza sativa), 3'ss of exon 1
lower sequence: EFJ26570 (Selaginella moellendorffii), 3'ss of exon 1
tttactgtattgcccccttttcatttctctcatttggattggttaaacgctatcaatatgtatttatcttgttaaaagaatctccttttctgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT
                                                    | |    |      | | |    |     |   | || || |    ||| | | ||   ||||||||||||||||  ||||||||||||||  |||||||||||   |||||| || || || || 
---------------------------------------------------gtgagagcgcgcggctatcgcgggatcttacgattcttgtgttgtgcagGTGCGCGGGCCGAAGAAGCATCTGAAGGTGCTCAATGCCCCCAAGCATTGGATGCTGAGCAAGCTCGGCGGTGCCTTC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|5003050|gb|AU068159.1|AU068159
EST:     TCGTTGTCCTCGCCCGCGCGCGAGGAGAACGCTAAACTCCGGTCAAGATG                         GCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGG
genomic: TCGTTGTCCTCGCCCGCGCGCGAGGAGAACGCTAAACTCCGGTCAAGATGgtgagatccg ... tgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGG
EST: gi|33685179|gb|CF313418.1|CF313418
EST:     TCGTTGTCCTCGCCCGCGCGCGAGGAGAACGCTAAACTCCGGTCAAGATG                         GCGAGGGGTTTGA
genomic: TCGTTGTCCTCGCCCGCGCGCGAGGAGAACGCTAAACTCCGGTCAAGATGgtgagatccg ... tgcttaatagGCGAGGGGTTTGA
EST: gi|5002967|gb|AU068076.1|AU068076
EST:     TCGTTGTCCTCGCCCGCGCGCGAGGAGANCGCTAAACTCCGGTCAAGATG                         GCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGG
genomic: TCGTTGTCCTCGCCCGCGCGCGAGGAGAACGCTAAACTCCGGTCAAGATGgtgagatccg ... tgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 64 (upstream exon), 58 (downstream exon)
Score: 21

GCCATTCTCTAGGGTTTATTCCACGCCTCCCGTCGCCGCCGCCGCCGCCGTCGTTGTCCTCGCCCGCGCGCGAGGAGAACGCTAAACTCCGGTCAAGATGgtgagatccg ... tgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aacgctatcaatatgtatttatcttgttaaaagaatctccttttctgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT
                                   tctccttttctgctt  CT-rich tract
     tatcaatatgtattta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tttactgtattgcccccttttcatttctctcatttggattggttaaacgctatcaatatgtatttatcttgttaaaagaatctccttttctgcttaatagGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTCAATGCCCCCAGCCATTGGATGCTTGACAAGCTTGGTGGAGCATTT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGTGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGCA
tttactg
- - - - - tgccccc
- - - - - - - - - tttcatt
- - - - - - - - - - - - - - -tcatttg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - tatgtat
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -aaaagaa