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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...atgttctgaaccaactgaaatcgatgttatgttgacgtactatgttggtttttgttactttcctttaatataccctttgttgtctggtattaattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACAATGTGTACATCTGCCGCTCTGGATCT

Basic information

species Oryza sativa
transcript LOC_Os02g53060.1
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os02g53060.2 (Oryza sativa), 3'ss of exon 1
lower sequence: GRMZM2G374881_T02 (Zea mays), 3'ss of exon 1
atg-ttctgaaccaactgaaatcgatgttatgttgacgtactatgttg--gtttttgttactttcctttaatataccctttgttgtctggtattaattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACAATGTGTACATCTGCCGCTCTGGATCT
 || || ||  ||   |     | |   ||  ||    || || | ||  || ||  ||| |||   ||     |     |    | |  |  |    || |||||||||||| || |||||||| || ||||||||||| ||  | || |||||||||||| | || ||||||||||||
-tgcttttgctcctgtttctgccaaa--taaatttcattattaagctgatgtattctttattttaaatttggccaagtgctaacatttcctgctccaactcagGAATGTATGTAGCCAACCGTGCTTCAGACAAGATTACCCAATTGACAGACAATGTGTACGTTTGTCGCTCTGGATCT

upper sequence: LOC_Os02g53060.2 (Oryza sativa), 3'ss of exon 1
lower sequence: GRMZM2G152775_T04 (Zea mays), 3'ss of exon 1
---atgttctgaaccaactgaaatcgatgttatgttgacgtactatgttggtttttgtt--actttcctttaatataccctttgttgtctggtattaattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACAATGTGTACATCTGCCGCTCTGGATCT
    ||||   | | ||  ||| ||| | ||| | ||| ||| | | ||  ||| | ||   |       ||||||    | ||||| ||| |   |   || |||||||||||| || |||||||| || |||||||||||||| || || |||||||||||  |||| ||||||||||||
ctcctgtttccaccaaatagaattcgttattaagctgatgtagtct-ttattttctatttggccaagtgctaatat-ttttgtgttgcctgctccaa---ctcagGAATGTATGTAGCCAACCGTGCTTCAGACAAGATTACTCAACTGACAGACAATGTGTATGTCTGTCGCTCTGGATCT

upper sequence: LOC_Os02g53060.2 (Oryza sativa), 3'ss of exon 1
lower sequence: GRMZM2G374881_T01 (Zea mays), 3'ss of exon 1
atg-ttctgaaccaactgaaatcgatgttatgttgacgtactatgttg--gtttttgttactttcctttaatataccctttgttgtctggtattaattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACAATGTGTACATCTGCCGCTCTGGATCT-----------------------
 || || ||  ||   |     | |   ||  ||    || || | ||  || ||  ||| |||   ||     |     |    | |  |  |    || |||||||||||| || |||||||| || ||||||||||| ||  | || |||||||||||| | || ||||||||||||                       
-tgcttttgctcctgtttctgccaaa--taaatttcattattaagctgatgtattctttattttaaatttggccaagtgctaacatttcctgctccaactcagGAATGTATGTAGCCAACCGTGCTTCAGACAAGATTACCCAATTGACAGACAATGTGTACGTTTGTCGCTCTGGATCTGTAAGTGTTGTTGTCTTCTAATG

upper sequence: LOC_Os02g53060.2 (Oryza sativa), 3'ss of exon 1
lower sequence: GRMZM2G152775_T03 (Zea mays), 3'ss of exon 1
---atgttctgaaccaactgaaatcgatgttatgttgacgtactatgttggtttttgtt--actttcctttaatataccctttgttgtctggtattaattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACAATGTGTACATCTGCCGCTCTGGATCT-----------------------
    ||||   | | ||  ||| ||| | ||| | ||| ||| | | ||  ||| | ||   |       ||||||    | ||||| ||| |   |   || |||||||||||| || |||||||| || |||||||||||||| || || |||||||||||  |||| ||||||||||||                       
ctcctgtttccaccaaatagaattcgttattaagctgatgtagtct-ttattttctatttggccaagtgctaatat-ttttgtgttgcctgctccaa---ctcagGAATGTATGTAGCCAACCGTGCTTCAGACAAGATTACTCAACTGACAGACAATGTGTATGTCTGTCGCTCTGGATCTGTAAGTGCCATTGTCTTCTAATG

upper sequence: LOC_Os02g53060.2 (Oryza sativa), 3'ss of exon 1
lower sequence: GLYMA04G08200.1 (Glycine max), 3'ss of exon 1
-----------------------------------------------------------------------------------atgttctgaaccaactgaaatcgatgttatgttgacgtactatgtt--ggtttttgttactttcctttaatataccctttgttgtctggtatta--attcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACAATGTGTACATCTGCCGCTCTGGATCT
                                                                                   ||  ||||    || |  || |    |  |||| | | | |  | |  | || | |||  |    | | |  ||   || ||||| |  | ||   | | | |||| ||||||| || ||||| || |||||||| || |||||||| ||||| ||||| ||| | || ||||||||||| 
gtacactctctttctcctttccaagccgatcccatattaatttagggttttcgttttttcttttgttttgttttgactgtttcattctctgggt-aattacaagct---tcttgtttaaggattgagattagattctcgttgttgatttgtgaagtaat-ttagttgtttattttttccactttcagGAGTGTATGTTGCCAACCGAGCATCGGACAAAATCACTCAGCTCACTGATAATGTATACGTGTGTCGCTCTGGATCG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|88330848|gb|CI636860.1|CI636860
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88421759|gb|CI621403.1|CI621403
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|218499779|gb|FG945547.1|FG945547
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|66924564|gb|CX111412.1|CX111412
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|58685118|gb|CK073805.1|CK073805
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTCTGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88721882|gb|CI743394.1|CI743394
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88573296|gb|CI664867.1|CI664867
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|58588610|gb|CF986918.1|CF986918
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88358083|gb|CI605008.1|CI605008
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|58692319|gb|CK081006.1|CK081006
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88325048|gb|CI646464.1|CI646464
EST:     GCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCANNGCACCG                         GAATGTATGTGGCAAACCGCGCGTCGGACAAGAT-ACTCAGCTA-CTGAC
genomic: GCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCA--GCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGAC
EST: gi|25799545|gb|CA755506.1|CA755506
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88309396|gb|CI655764.1|CI655764
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|38477245|gb|CF961378.1|CF961378
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|218498714|gb|FG963991.1|FG963991
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCA-CACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88390264|gb|CI605717.1|CI605717
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|218498950|gb|FG948071.1|FG948071
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|38472737|gb|CF956869.1|CF956869
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88720636|gb|CI743935.1|CI743935
EST:     TGCTACGACGGCGGCGTCGTCCTCGCCGCCGATNTC-AGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCACCTAACTGACA
genomic: TGCTACGACGGCGGCGTCGTCCTCGCCGCCGAT-TCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88584075|gb|CI701601.1|CI701601
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|33803228|gb|CF327486.1|CF327486
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88302060|gb|CI667906.1|CI667906
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|218504275|gb|FG947024.1|FG947024
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88338208|gb|CI633327.1|CI633327
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|13165514|gb|AU174311.1|AU174311
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88342591|gb|CI655593.1|CI655593
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|88692730|gb|CI736305.1|CI736305
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|218486985|gb|FG965418.1|FG965418
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GGATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
EST: gi|14191702|gb|AU183913.1|AU183913
EST:     CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCG                         GAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA
genomic: CTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 71 (upstream exon), 71 (downstream exon)
Score: 45

GACAATCCCACCTCCGGGGAGCACCGGATGGGGACCACCATCGTCGGCGTCTGCTACGACGGCGGCGTCGTCCTCGCCGCCGATTCCAGAACCAGCACCGgtatgcctcg ... taattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACAATGTGTACATCTGCCGCTCTGGATCT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tggtttttgttactttcctttaatataccctttgttgtctggtattaattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACAATGTGTACATCTGCCGCTCTGGATCT
                                          tattaat  putative branch site (score: 45)
                                                ttctt  CT-rich tract
             tttcctttaatata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
atgttctgaaccaactgaaatcgatgttatgttgacgtactatgttggtttttgttactttcctttaatataccctttgttgtctggtattaattcttagGAATGTATGTGGCAAACCGTGCGTCGGACAAGATTACTCAGCTAACTGACAATGTGTACATCTGCCGCTCTGGATCT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TCAGCT
-tgttctg
- - - - aaccaac
- - - - - - - - - - - - - -tatgttg
- - - - - - - - - - - - - - atgttga
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tgtctgg