ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtactgtacagttccacgagaaatattattaattgtttgggttttggtatgtcaggacatccttgatcaacttacttggttgtacatatagGCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCAATTTAATTAAGCATCCAAAGTTCATCAGTGATATCAAGTCCGCTATCG

Basic information

species	Oryza sativa
transcript	LOC_Os02g05630.4
intron #	1
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os02g05630.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GRMZM2G166035_T02 (Zea mays), 3'ss of exon 2

----gtactgtacagttccacgagaaatattattaatt-gtttgggttttggtatgtcag--------gacatccttgatcaacttactt----ggt--------------tgtacatatagGCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCAATTTAATTAAGCATCCAAAGTTCATCAGTGATATCAAGTCCGCTATCG
    ||| ||   |||  ||   |||||  ||| ||||   |||| | ||   ||| |||        ||   | |||||    | ||||    |||              |||   | |||| || || ||||   |||| ||||| || || |||||||| ||||||||||||||||| || || ||||||||||||| ||||| |||| ||||||| |
gtatgtattgcctagtgacatctgaaatcatatcaattcagttggttcttctaatgccagcgctccacgatggcattgatattttcacttataaggtgagcatgttttttctgtttctgtagGTCACGGAGGAGTCAGAGCAGCTGAGTACGTTAAGCAGCATCTTTTCAGCAATTTAATCAAACACCCAAAGTTCATCACTGATACCAAGGCCGCTATTG

upper sequence: LOC_Os02g05630.1 (Oryza sativa), 3'ss of exon 2
lower sequence: GRMZM5G829894_T02 (Zea mays), 3'ss of exon 1

----gtactgtacagttccacgagaaatattattaattgtttgggttttggta-tgtcaggacatccttgatcaacttacttg-----gttgtaca------------------------tatagGCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCAATTTAATTAAGCATCCAAAGTTCATCAGTGATATCAAGTCCGCTATCG
    ||| |    ||   ||   ||| |  ||| ||||     ||||||  || || ||  || ||  |||| || ||  | |     ||||||                          | |||| ||||| ||||  ||||| ||||| ||||| |||||||||||||||||||||||||| || || ||||||||||||| ||||| |||| | |||||||
gtatgtattcgctagcgacatctgaagtgatatcaattcccgcggttttcctaatgccaccac-tctctgatgaagttggtagtttgagttgtaaggttgagggcatggttttctgtttctgtagGTCATGGAGGAGTCCGAGCAGCTGAGTATGTTAAGCAGCACCTTTTCAGCAATTTAATCAAACACCCAAAGTTCATCACTGATACCAAGGCTGCTATCG

upper sequence: LOC_Os02g05630.1 (Oryza sativa), 3'ss of exon 2
lower sequence: Vv04s0008g07320.t01 (Vitis vinifera), 3'ss of exon 3

----gtactgtacagttccacgagaaatattattaattgtttgggttttggt-----atgtcaggacatccttgatcaacttacttggttgtacatat-agGCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCAATTTAATTAAGCATCCAAAGTTCATCAGTGATATCAAGTCCGCTATCG
    || | |  |      | | |   ||   || || | | | | ||         || | ||   || ||||   |   | || |  ||    | | ||| ||||| || |||||||| ||||||||||| || ||  ||||||| || ||||| || | |||||||||||||||   |||||  || || ||||| |
ccatgtgccgctcgcaaatatgggtt-tagctttgatagatcgagcttccaatgtcaatattagataatgcttgcgtattcttctggtatggttgtctcagGTCATGGAGGTGCTCGAGCAGCTGAATATGTTAAACAAAACCTTTTTAGTAATTTGATCAGGCATCCAAAGTTCATTTCTGATACAAAATCAGCTATAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|5003494|gb|AU068643.1|AU068643

EST:     AATCGATGGTGTTGATGGAGAGACCATTGGATTATTTGGCGTATTCGACG                         GCCATGGCGGA-CTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCA
genomic: AATCGATGGTGTTGATGGAGAGACCATTGGATTATTTGGCGTATTCGACGgtactgtaca ... gtacatatagGCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCA

EST: gi|33680351|gb|CF308590.1|CF308590

EST:     AATCGATGGTGTTGATGGAGAGACCATTGGATTATTTGGCGTATTCGACG                         GCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCA
genomic: AATCGATGGTGTTGATGGAGAGACCATTGGATTATTTGGCGTATTCGACGgtactgtaca ... gtacatatagGCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCA

EST: gi|218495837|gb|FG960245.1|FG960245

EST:     GGCGTA-TCGACG                         GCCATGGCGGAGCTCGAGCGGCTGAATATGTCAATGCAGCACCTTATTCAG
genomic: GGCGTATTCGACGgtactgtaca ... gtacatatagGCCATGGCGGAGCTCGAGCGGCTGAATATGTCAA-GCAGCACCTT-TTCAG

EST: gi|33675133|gb|CF303372.1|CF303372

EST:     AATCGATGGTGTTGATGGAGAGACCATTGGATTATTTGTCGTATTCGACG                         GCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCA
genomic: AATCGATGGTGTTGATGGAGAGACCATTGGATTATTTGGCGTATTCGACGgtactgtaca ... gtacatatagGCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 75 (upstream exon), 74 (downstream exon)
Score: 29

GCAAGTGCTCCTGGGAAAAGGGCTTCAATGGAGGATTTCTACGAGACGAGAATCGATGGTGTTGATGGAGAGACCATTGGATTATTTGGCGTATTCGACGgtactgtaca ... gtacatatagGCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCAATTTAATTAAGCATCCAAAGTTCATCAGTGATATCAAGTCCGCTATCG

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atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttgggttttggtatgtcaggacatccttgatcaacttacttggttgtacatatagGCCATGGCGGAGCTCGAGCGGCTGAATATGTCAAGCAGCACCTTTTCAGCAATTTAATTAAGCATCCAAAGTTCATCAGTGATATCAAGTCCGCTATCG