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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtttgttttcctgcgagtgctctctaatttgcactgattaagctttgatgatgcttgatttgtggtttgattgttcttcttgttgttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAAGGAGCTCAAGGATCTGCAGAAGGATCCCCCTACATCATGCAGTGCAG

Basic information

species Oryza sativa
transcript LOC_Os01g46926.1
intron # 1
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|86849404|gb|CI299631.1|CI299631
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|55431330|gb|CV728679.1|CV728679
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88729958|gb|CI753573.1|CI753573
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|55438244|gb|CV732417.1|CV732417
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|38471522|gb|CF955653.1|CF955653
EST:     GTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: GTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|86902035|gb|CI300274.1|CI300274
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|33671124|gb|CF299363.1|CF299363
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|38565753|gb|CK085206.1|CK085206
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88745084|gb|CI768444.1|CI768444
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|163920365|gb|EG711740.1|EG711740
EST:     GGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: GGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|117238030|gb|CT862028.1|CT862028
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|58594014|gb|CF992322.1|CF992322
EST:     GGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAGTTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: GGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|3759930|gb|C97188.1|C97188
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|2310784|gb|C26939.1|C26939
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88377844|gb|CI637978.1|CI637978
EST:     GAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: GAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88346503|gb|CI637349.1|CI637349
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|66913588|gb|CX100436.1|CX100436
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88570585|gb|CI661958.1|CI661958
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88730304|gb|CI753918.1|CI753918
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|58597143|gb|CK007671.1|CK007671
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88308997|gb|CI654419.1|CI654419
EST:     GAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: GAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|163928906|gb|EG709759.1|EG709759
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88762126|gb|CI771682.1|CI771682
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|58594773|gb|CF993081.1|CF993081
EST:     CGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         T
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatag-
EST: gi|58594773|gb|CF993081.1|CF993081
EST:     CGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         T
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatag-
EST: gi|88718883|gb|CI742393.1|CI742393
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|86898750|gb|CI296063.1|CI296063
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88730675|gb|CI754288.1|CI754288
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88294636|gb|CI602356.1|CI602356
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|86898783|gb|CI296080.1|CI296080
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|88692482|gb|CI736057.1|CI736057
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|58603424|gb|CK013952.1|CK013952
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTGGAGAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|55413329|gb|CV725705.1|CV725705
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAGAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|87019040|gb|CI297348.1|CI297348
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|3759748|gb|C97006.1|C97006
EST:     AAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|58591683|gb|CF989991.1|CF989991
EST:     GGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: GGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|38472298|gb|CF956429.1|CF956429
EST:     GGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: GGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
EST: gi|58672051|gb|CK060737.1|CK060737
EST:     AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAG                         GAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA
genomic: AGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 75 (upstream exon), 70 (downstream exon)
Score: 227

CTCTTGGTTCCTTTCCCCCAACTCCATTATTGATTCTTGCAAGGAGGAAGAGCAGCTAGAGGCGAGGCAAGAAAAGAAGTGAAATCTCTCCGTTAGACAGgtttgttttc ... ttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAAGGAGCTCAAGGATCTGCAGAAGGATCCCCCTACATCATGCAGTGCAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agctttgatgatgcttgatttgtggtttgattgttcttcttgttgttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAAGGAGCTCAAGGATCTGCAGAAGGATCCCCCTACATCATGCAGTGCAG
                        gtttgat  putative branch site (score: 227)
                                 ttcttcttgttgtt  putative PPT
              ttgattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtttgttttcctgcgagtgctctctaatttgcactgattaagctttgatgatgcttgatttgtggtttgattgttcttcttgttgttggagatagGAAGAGGAAAAGCAAGGGGGAATTGGGGATGGCGTCAAAGAGGATACAGAAGGAGCTCAAGGATCTGCAGAAGGATCCCCCTACATCATGCAGTGCAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGCAG