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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtttgtggctttcctaccttcatttatgctaatatgtgtgcatctatgccctcgtattccttcactgttaacctctgtgtgacacgttttgcttatttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGATCTCAAGAACAAGAATAAATACGAT

Basic information

species Glycine max
transcript GLYMA09G40060.2
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GLYMA09G40060.2 (Glycine max), 3'ss of exon 5
lower sequence: AT1G01820.1 (Arabidopsis thaliana), 3'ss of exon 5
gtttgtggctttcctaccttcatttatgctaatatgtgtgcatctatgccctcgtattccttcactgttaacctctgtgtgacacgttttgcttatttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGATCTCAAGAACAAGAATAAATACGAT
                  |   || |  ||   ||       ||| |  | ||   |||     |  |    |    ||| | |  ||   | | |||    || ||||||||||||| || || || |||||||| ||||||||||| |||||  |   ||||||| ||||| || | 
-----------------gtctgttca--ctgccattgaattgtctcttacttctggttcaaatgcaataccgcataatgtaataatttcgacatgttt----agGTTGGGGAGCTTGGTAGGCTGTCAGCATCAATTAAGAAGTTAGAAAAAGAGATAGGGAACAAGGATAAACACCAG

upper sequence: GLYMA09G40060.2 (Glycine max), 3'ss of exon 5
lower sequence: Vv15s0048g01890.t01 (Vitis vinifera), 3'ss of exon 5
gtttgtggctttcctacctt------catttatgctaatatgtgtgcatctatgccctcgtattccttcactg----ttaacctct---------gtgtgacacgttttgcttatttgata-agCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGATCTCAAGAACAAGAATAAATACGAT
|||||| | ||||  || ||      || |||  ||  | | ||   || | |  ||  |  ||| ||  ||     || |  | |         ||||  | | |    | | |||  |  |||||||||| |||||||| || |||||||||||||||||||||||||| || || |||   |   |||||||  | 
gtttgttgttttcaaacttttctcaacaattaaacttgtgtatgctaatttttctccctgatttcttttcctagtttttgatattttattttgatgtgttccccctcccccctctttatttcagCTTGGGGAACTTGGAAGGCTTTCTGCATCAATGAAGAAGTTAGAGAAGGAGCTTAAGGGTAGTGATAAATATCAA
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|254336246|gb|GR842425.1|GR842425
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGA
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTT-GGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGA
EST: gi|298179034|gb|HO024769.1|HO024769
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTT
EST: gi|58015330|gb|CX702072.1|CX702072
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|254343225|gb|GR856929.1|GR856929
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|254324273|gb|GR840590.1|GR840590
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|7691912|gb|AW760029.1|AW760029
EST:     GTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: GTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|192323627|gb|FK019038.1|FK019038
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|213589574|gb|DB973217.1|DB973217
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|254343224|gb|GR856928.1|GR856928
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|151394416|gb|EV264287.1|EV264287
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGCGCCACCTCGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|8403460|gb|BE059094.1|BE059094
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|120492016|gb|EH220183.1|EH220183
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|254324274|gb|GR840591.1|GR840591
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|7686223|gb|AW756934.1|AW756934
EST:     TATCTCTNTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGA
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGA
EST: gi|13312196|gb|BG405856.1|BG405856
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|19348498|gb|BM893030.1|BM893030
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAA
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAA
EST: gi|193352101|gb|FK316382.1|FK316382
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|58015584|gb|CX702326.1|CX702326
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|254330639|gb|GR841904.1|GR841904
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|7639927|gb|AW734239.1|AW734239
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|21888536|gb|BQ741749.1|BQ741749
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|19269910|gb|BM886166.1|BM886166
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|254336245|gb|GR842424.1|GR842424
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|22927124|gb|BU544263.1|BU544263
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|27425936|gb|CA937456.1|CA937456
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|48575471|gb|CO036420.1|CO036420
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|6724567|gb|AW308966.1|AW308966
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|19348375|gb|BM892907.1|BM892907
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|254335608|gb|GR847136.1|GR847136
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAG
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAG
EST: gi|6071462|gb|AW100849.1|AW100849
EST:     CTCTTTACTGTTGGTTGGGATCCTCTTTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: CTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|192316781|gb|FK006484.1|FK006484
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|23057464|gb|BU578138.1|BU578138
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|7691181|gb|AW759313.1|AW759313
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|213607370|gb|DB967079.1|DB967079
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
EST: gi|22932789|gb|BU549928.1|BU549928
EST:     TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAG                         CTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT
genomic: TATCTCTTTACTGTTGGTTGGGATCCTCGGTGTGTGCCACCTTGGTTGAGgtttgtggct ... atttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGAT


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cctcgtattccttcactgttaacctctgtgtgacacgttttgcttatttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGATCTCAAGAACAAGAATAAATACGAT
                tgttaac  putative branch site (score: 2)
                                          cttattt  putative PPT
                                           ttatttgataa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtttgtggctttcctaccttcatttatgctaatatgtgtgcatctatgccctcgtattccttcactgttaacctctgtgtgacacgttttgcttatttgataagCTTGGGGAGCTTGGAAGACTCTCTGCATCAATGAAGAAGTTAGAGAAAGATCTCAAGAACAAGAATAAATACGAT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAC