Skip to another splice site:
1  
 5'  3'   		2  
 5'  3'   		3  
 5'  3'   		4  
 5'  3'   		5  
 5'  3'   		6  
 5'  3'   		7  
 5'  3'   		8  
 5'  3'   		9  
 5'  3'   		10  
 5'  3'   		11  
 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tatttgaattgaaaaatgcagataaaaaatttctcacgattactatatataaattgtaatatactgttttgtatgtggcatatggtggatttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCATGCATTAGGCCATGTGACCAGCAATGTCTCATTTGCTGCAGTTGCTGTTT

Basic information

species Glycine max
transcript GLYMA07G32190.1
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GLYMA07G32190.1 (Glycine max), 3'ss of exon 4
lower sequence: Vv10s0003g00300.t01 (Vitis vinifera), 3'ss of exon 4
tatttgaattgaaaaatgcagataaaaaatttctcacgattactatatataaattgtaata--tactg-ttttgtatgtggcatatgg----tggatttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCATGCATTAGGCCATGTGACCAGCAATGTCTCATTTGCTGCAGTTGCTGTTT
         || | || ||  |||     | |   |    |  |    || | |||||     || || |||||    || |||  ||    ||| |||   |||||||||||||||| ||||| |||||  ||||||| || || ||||| ||||||||  ||||||||||||| ||||||||||| ||||| |||||||| || |
-------tctgtagaaagccaatattgtttcttctatatatgatgatgatgacttgtagcccttattgattttgggaatgacatgcggcttgtggttttccttgtagCCTATAGACTCAAACCTCCTGAAACTGCTCATCCCCGTTGCTGTATGTCATGCTCTAGGCCATGTGACTAGCAATGTCTCCTTTGCAGCAGTTGCCGTCT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|7692862|gb|AW760961.1|AW760961
EST:     TTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: TTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|209720817|gb|BW677391.1|BW677391
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|120534376|gb|EH262509.1|EH262509
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCGGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|7691041|gb|AW759175.1|AW759175
EST:     CCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: CCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|207691594|gb|GD675269.1|GD675269
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|213615350|gb|DB963544.1|DB963544
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|13480400|gb|BG509743.1|BG509743
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCGGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|20448694|gb|BQ252818.1|BQ252818
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|120534368|gb|EH262501.1|EH262501
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCGGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|7796788|gb|AW782182.1|AW782182
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|213589339|gb|DB961672.1|DB961672
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|20448800|gb|BQ252924.1|BQ252924
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|213587747|gb|DB955609.1|DB955609
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|209721814|gb|BW680013.1|BW680013
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGT
EST: gi|120531507|gb|EH259640.1|EH259640
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAAACTACTGAAGTTGCTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCAT
EST: gi|23063798|gb|BU578627.1|BU578627
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCGGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|15285164|gb|BI469055.1|BI469055
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|11412623|gb|BF424634.1|BF424634
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|26269548|gb|CA820611.1|CA820611
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|17519248|gb|BM188290.1|BM188290
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCGGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|6481941|gb|AW201212.1|AW201212
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|213587892|gb|DB959716.1|DB959716
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCTTTCCAAAGCGTGCT                         CCTATAGACTCCA-CCNTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCA
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACC-TACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCA
EST: gi|7796582|gb|AW781979.1|AW781979
EST:     GTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|20814778|gb|BQ299256.1|BQ299256
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|17153411|gb|BM143344.1|BM143344
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCGGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|15337762|gb|BI498418.1|BI498418
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|26058933|gb|CA801847.1|CA801847
EST:     GAGTGGCGTACTGTCTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCC
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCC
EST: gi|16104872|gb|BI892612.1|BI892612
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|7691923|gb|AW760040.1|AW760040
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|213593376|gb|DB973569.1|DB973569
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|193705590|gb|FK643540.1|FK643540
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|23059611|gb|BU578385.1|BU578385
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|24137046|gb|BU927556.1|BU927556
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCGGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|213590584|gb|DB973329.1|DB973329
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCAT
EST: gi|15287351|gb|BI471242.1|BI471242
EST:     GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCT                         CCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAG
genomic: GAGTGGCGTACTGTTTGGTGAGCTGGGCCGTGGGCCTTCCAAAGCGTGCTgtaagtcaag ... ttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAG


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tatataaattgtaatatactgttttgtatgtggcatatggtggatttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCATGCATTAGGCCATGTGACCAGCAATGTCTCATTTGCTGCAGTTGCTGTTT
                                            tttttctgt  CT-rich tract
     aaattgtaatatact  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tatttgaattgaaaaatgcagataaaaaatttctcacgattactatatataaattgtaatatactgttttgtatgtggcatatggtggatttttctgtagCCTATAGACTCCAACCTACTGAAGTTGCTCATTCCAGTGGCTGTGTGTCATGCATTAGGCCATGTGACCAGCAATGTCTCATTTGCTGCAGTTGCTGTTT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGTT
- - - - - - - aatgcag
- - - - - - - - - - - -aaaaaat
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - gtatgtg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tggtgga