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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtatgttgcttgacattctttcggttttggcatttaaaattcgttctgtcaggagttagatattcttacctatccatgcatcactcgtgtgttaccttcctgtatgcatgtatctatcatgtatattttagtgtagcatcatgcattacaaaaatccgtttagtttttgggcctaatgtatgtgattaaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATTGCACAACCAAAG

Basic information

species Glycine max
transcript GLYMA08G24000.1
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GLYMA08G24000.1 (Glycine max), 3'ss of exon 3
lower sequence: LOC_Os02g11050.1 (Oryza sativa), 3'ss of exon 3
gtatgttgcttgacattctttcggttttggcatttaaaattcgttctgtcaggagttagatattcttacctatccatgcatcactcgtgtgttaccttcctgtatgcatgtatctatcatgtatattttagtgtagcatc-atgcattacaaaaatccgtttagtttttgggcctaatgtatgtgattaaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATTGCACAACCAAAG
                                                                                                                | | | || || |||| | ||||  ||  ||||||  || | | |   ||           || |   |||  | | ||  |  ||||||||||  | || ||||||||||| ||||| ||||| |||||||||||||| |||||||||
---------------------------------------------------------------------------------------------------------------gtttgt-atata-atttcaatgtactattgatgcatcgcatatagcacatt-----------ctgactcatg-aactcaacttttagGTCATTGAACTTCCTATCAAACATCCAGAGCTATTTGAGAGTCTTGGAATTGCCCAACCAAAG

upper sequence: GLYMA08G24000.1 (Glycine max), 3'ss of exon 3
lower sequence: GRMZM2G107362_T03 (Zea mays), 3'ss of exon 3
gtatgttgcttgacattctttcggttttggcatttaaaattcgttctgtcaggagttagatattcttacctatccatgcatcactcgtgtgttaccttcctgtatgcatgtatctatcatgtatattttagtgtagcatcatgcattacaaaaatccgtttagtttttgggcctaatgtatgtgattaaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATTGCACAACCAAAG-------------------------------------
                                                                                                                        || | ||||    ||  |       | |  |||||  |   | |        || || | ||  | | ||  | |||||||||||| | |||||||||||||| || |||||||| || ||||||||||| |||||||||                                     
------------------------------------------------------------------------------------------------------------------------gtttgttttgaactaaaaagcactgtcattaaaatatgcacatt--------ctgatttgtg-aactcaactttcagGTCATTGAGCTTCCAATCAAACATCCGGAACTGTTTGAGAGCCTTGGAATTGCGCAACCAAAGGTTTGTTTTCTTTTTTGAAGACCATCTTAACCTTTTG

upper sequence: GLYMA08G24000.1 (Glycine max), 3'ss of exon 3
lower sequence: GRMZM2G107362_T04 (Zea mays), 3'ss of exon 3
gtatgttgcttgacattctttcggttttggcatttaaaattcgttctgtcaggagttagatattcttacctatccatgcatcactcgtgtgttaccttcctgtatgcatgtatctatcatgtatattttagtgtagcatcatgcattacaaaaatccgtttagtttttgggcctaatgtatgtgattaaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATTGCACAACCAAAG
                                                                                                                        || | ||||    ||  |       | |  |||||  |   | |        || || | ||  | | ||  | |||||||||||| | |||||||||||||| || |||||||| || ||||||||||| |||||||||
------------------------------------------------------------------------------------------------------------------------gtttgttttgaactaaaaagcactgtcattaaaatatgcacatt--------ctgatttgtg-aactcaactttcagGTCATTGAGCTTCCAATCAAACATCCGGAACTGTTTGAGAGCCTTGGAATTGCGCAACCAAAG

upper sequence: GLYMA08G24000.1 (Glycine max), 3'ss of exon 3
lower sequence: GRMZM2G137528_T01 (Zea mays), 3'ss of exon 3
gtatgttgcttgacattctttcggttttggcatttaaaattcgttctgtcaggagttagatattcttacctatccatgcatcactcgtgtgttaccttcctgtatgcatgtatctatcatgtatattttagtgtagcatcatgcattacaaaaatccgtttagtttttgggcctaatgtatgtgattaaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATTGCACAACCAAAG
                                                                                                                        || | ||||    ||  ||   | || || |||||  |   | |        || || | ||  |   ||  | |||||||||||| | |||||||||||||| || || ||||| || ||||||||||| |||||||||
------------------------------------------------------------------------------------------------------------------------gtttgttttgaactaaaatgcagtatcaccaaaatatgcacatt--------ctgatttgtg-aacacaactttcagGTCATTGAGCTTCCAATCAAACATCCGGAACTATTTGAGAGCCTTGGAATTGCCCAACCAAAG

upper sequence: GLYMA08G24000.1 (Glycine max), 3'ss of exon 3
lower sequence: AT5G19990.1 (Arabidopsis thaliana), 3'ss of exon 3
gtatgttgcttgacattctttcggttttggcatttaaaattcgttctgtcaggagttagatattcttacctatccatgcatcactcgtgtgttaccttc-ctgtatgcatgtatc-tatcatgtatattttagtgtagcatcatgcattacaaaaatccgtttagtttttgggcctaatgtatgtgattaaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATTGCACAACCAAAG
                                                                           |||| |      || |  |||    ||  | |    ||   |||    ||| |  |  ||| |  |||| | ||   ||  |   | || | | |    ||    | | || || |     |||||||||||| | |||||||| ||||||||  |||||||   |||||||||||| || ||||||
-------------------------------------------------------------------------gtatgcttagtgaatgagaaaccgaaactaaaagtcactacagtattggctatctcctc-tgttgtttcatcctttt--aatgtttatataatatctatatct----tttctggtttat----cagGTCATTGAGCTGCCAATCAAGCATCCTGAATTGTTTGAGTCTCTTGGAATTGCGCAGCCAAAG

upper sequence: GLYMA08G24000.1 (Glycine max), 3'ss of exon 3
lower sequence: Vv09s0002g05800.t01 (Vitis vinifera), 3'ss of exon 3
gtatgttgcttgacattctttcggttttggcatttaaaattcgttctgtcagg--agttagatattcttacctatccatgcatcactcgtgtgttaccttcctgtatg-catgtatctatcatgtatattttagtgtagcatcatgcattacaaaaatc-cgtttagtttttgggcctaatgtatgtgat--taaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATTGCACAACCAAAG
                         |||| | |||   |    ||||  || |  | ||   |||| |||         ||| ||||  |   ||   ||    || |   |  ||   | || ||||  | ||    | |||    |||   |   || | ||| | |||    |||  | | |||  |  | |   | |||||||||||| | ||||| ||||| ||||| |||||||| ||||||||||| || |||||||||
------------------------gtttgtcctttg--accaattcttacatgttatttcattattattattaccattagcagcacttttaccttgatttaagttaggatttaaattcttaatctatagataagaaaggtatctcagatttttattttctcattttggttt---accttgtttttgttgtcttgatctttcagGTCATTGAGCTTCCAATTAAACACCCTGAACTGTTTGAGAGTCTTGGAATAGCTCAACCAAAG

upper sequence: GLYMA08G24000.1 (Glycine max), 3'ss of exon 3
lower sequence: Vv04s0008g04690.t01 (Vitis vinifera), 3'ss of exon 3
gtatgttgcttgacattctttcggttttggcatttaaaattcgttctgtcaggagttagatattcttacctatccatgcatcactcgtgtgttaccttcctgtatgcatgtatctatcatgtatattttagtgtagcatcatgcattacaaaaatccgtttag-tttttgggcctaatgtatgtgattaaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATTGCACAACCAAAG
                                                                                                                    | |  |||||   ||  |    |  ||  |||      |  ||| |  |  ||  | || |||  |||  | ||   | ||||| |||||| | |||||||| ||||||||  | |||||||||||||| || || |||||||||
-------------------------------------------------------------------------------------------------------------------gttagctatatgagagcttc---ttctgatttatttgttttggttgaaatccttctgtcttatgc-tgtcttaaacccttcagGTTATTGAGCTTCCAATCAAGCATCCTGAATTATTTGAAAGTCTTGGTATAGCTCAACCAAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|120493760|gb|EH221927.1|EH221927
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTACAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAA
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAA
EST: gi|16346043|gb|BI971638.1|BI971638
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|16281730|gb|BI944638.1|BI944638
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|254349061|gb|GR860333.1|GR860333
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAAAT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAA-T
EST: gi|213596302|gb|DB960231.1|DB960231
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAG
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAG
EST: gi|192327448|gb|FK019605.1|FK019605
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|37995461|gb|CF807050.1|CF807050
EST:     TTACGACATGATTGGTGGTTTAGACCCAGCAA-TTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: TTACGACATGATTGGTGGTTTAGACC-AGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|120533815|gb|EH261948.1|EH261948
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|26047693|gb|CA784406.1|CA784406
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|7028501|gb|AW458284.1|AW458284
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCANATTAAAGAAATAAAAGAT                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGACAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|254333837|gb|GR848755.1|GR848755
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|21594964|gb|BQ610324.1|BQ610324
EST:     CTTACGACATGATTGGTGGGTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|254323845|gb|GR840378.1|GR840378
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|37994568|gb|CF806272.1|CF806272
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|16281214|gb|BI944372.1|BI944372
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|151411634|gb|EV281445.1|EV281445
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|254325633|gb|GR838514.1|GR838514
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|15662663|gb|BI700034.1|BI700034
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|16280447|gb|BI943967.1|BI943967
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|151412070|gb|EV281882.1|EV281882
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|16284142|gb|BI945928.1|BI945928
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|7686556|gb|AW757204.1|AW757204
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAGAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|18734930|gb|BM528466.1|BM528466
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|120531685|gb|EH259818.1|EH259818
EST:     CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGACAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
EST: gi|6134230|gb|AW132623.1|AW132623
EST:     CTTACGACATGATTGGTGGTTTAAACCANCAAATTAAAGAAATAAAAGAA                         GTCATTGANTTACCAATCAAACATCCTGA
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGA
EST: gi|151394633|gb|EV264504.1|EV264504
EST:     CTTACGACATGATTGGTGGCTTAGACCAGCAAATTAAAGAAATAAAAGAG                         GTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT
genomic: CTTACGACATGATTGGTGGTTTAGACCAGCAAATTAAAGAAATAAAAGAGgtatgttgct ... aaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATT


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gcattacaaaaatccgtttagtttttgggcctaatgtatgtgattaaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATTGCACAACCAAAG
                            gcctaat  putative branch site (score: 3)
                tttagttttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatgttgcttgacattctttcggttttggcatttaaaattcgttctgtcaggagttagatattcttacctatccatgcatcactcgtgtgttaccttcctgtatgcatgtatctatcatgtatattttagtgtagcatcatgcattacaaaaatccgtttagtttttgggcctaatgtatgtgattaaatgtacagGTCATTGAGTTACCAATCAAACATCCTGAGCTGTTTGAAAGTCTTGGAATTGCACAACCAAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGTT