ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TGAGCGGCCAGCAACCCCCCCTGATGGAGAGGAAGAATTTACTGATGATGATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAAgtgagtactgctaatctgtctttgccatgactagctaaatacactgcctagttccaccagtcgattgattgactttccaccattgaattcatgagtctcc...

Basic information

species	Zea mays
transcript	GRMZM2G171660_T02
intron #	3
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G171660_T03 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os04g41910.1 (Oryza sativa), 5'ss of exon 3

TGAGCGGCCAGCAACCCCCCCTGATGGAGAGGAAGAATTTACTGATGATGATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAAgtgagtactgctaatctgtctttgccatgactagctaaatacactgcctagttccaccagtcgattgattgactttccaccattgaattcatga-gtctcc-
||| |||||||||||||| || ||||| ||||| |||||||||||||||||||| ||  ||||||| ||||| |||    |||||||||||||||||||||||||| || | || ||    ||   | |   |  ||   |       |  ||     |||    |||||     |     ||| | |  ||   || |   
TGACCGGCCAGCAACCCCACCAGATGGCGAGGACGAATTTACTGATGATGATGGTACTGTTTACAAGTGGGATCGTGTCCTGAGGGCATGGGTTCCTCAAgtgagtgcttccaacctcctgttcttaggttcatttactcatgacttttgtttttggtagtt--ctgattttgcctgagatattaattagatattgtgtttt

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|91054747|gb|EB165165.1|EB165165

EST:     ATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAA                         AATGATGCATCTGGTAGCAAGGAAGATTACGCTGTTGAAGAGATGACTTTT
genomic: ATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAAgtgagtactg ... gtatttatagAATGATGCATCTGGTAGCAAGGAAGATTACGCTGTTGAAGAGATGACTTTT

EST: gi|149070733|gb|EE159146.2|EE159146

EST:     ATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAA                         AATGATGCATCTGGTAGCAAGGAAGATTACGCTGTTGAAGAGATGACTTTT
genomic: ATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAAgtgagtactg ... gtatttatagAATGATGCATCTGGTAGCAAGGAAGATTACGCTGTTGAAGAGATGACTTTT

EST: gi|148966210|gb|EE017166.2|EE017166

EST:     ATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAA                         AATGATGCATCTGGTAGCAAGGAAGATTACGCTGTTGAAGAGATGACTTTT
genomic: ATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAAgtgagtactg ... gtatttatagAATGATGCATCTGGTAGCAAGGAAGATTACGCTGTTGAAGAGATGACTTTT

EST: gi|76909704|gb|DV163493.1|DV163493

EST:     ATGGCACCATTTACAAATGGGACCGTACTTTGAGGGGATGGGTTCCTCAA                         AATGATGCATCTG
genomic: ATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAAgtgagtactg ... gtatttatagAATGATGCATCTG

EST: gi|149004610|gb|EE039124.2|EE039124

EST:     ATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAA                         AATGATGCATCTGGTAGCAAGGAAGATTACGCTGTTGAAGAGATGACTTTT
genomic: ATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAAgtgagtactg ... gtatttatagAATGATGCATCTGGTAGCAAGGAAGATTACGCTGTTGAAGAGATGACTTTT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 55

TGAGCGGCCAGCAACCCCCCCTGATGGAGAGGAAGAATTTACTGATGATGATGGCACCATTTACAAATGGGACCGTACTTTGAGGGCATGGGTTCCTCAAgtgagtactg ... gtatttatagAATGATGCATCTGGTAGCAAGGAAGATTACGCTGTTGAAGAGATGACTTTTGCAGTTGAGGAGGAAATATTTCAGGCACCCGATATCCTGGGTCCTGCAT

Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 54

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 59