Skip to another splice site:
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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TGAGAGCTTTCCTGCATCTCTCTTGGATCTACCTGCTGTGGTGGAGTCATACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgcatgtttaatgtatgtcacctgtttatttattaaacttcgtatcttcaaatcgtccactgaaaacaaactgaaacag

Basic information

species Zea mays
transcript GRMZM2G147614_T01
intron # 3
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G147614_T01 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os05g28010.1 (Oryza sativa), 5'ss of exon 2
TGAGAGCTTTCCTGCATCTCTCTTGGATCTACCTGCTGTGGTGGAGTCATACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgcatgtttaatgtatgtcacctgtttatttattaaacttcgtatcttcaaatcgtccactgaaaacaaactgaaacag
||| |||||||||||||| ||||||||||| ||  |||| |||||||||||||| |||||||||||||| || ||||||||||| |||||| ||||||||||||| |   | ||||  |||||   | ||||||   ||||||   | |  | || | |   |||  |  | |     |  |||||
TGAAAGCTTTCCTGCATCACTCTTGGATCTCCCAACTGTTGTGGAGTCATACAAGACATATGATGATTCAGTGCTAATTAAAACAGCTGATATTGGTCAGgtaaa-gcataggttttgtgtataagatctgttt---tattaactgtaggcttttaagactctcctgttgagat----tataacag

upper sequence: GRMZM2G147614_T01 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os01g46020.1 (Oryza sativa), 5'ss of exon 2
TGAGAGCTTTCCTGCATCTCTCTTGGATCTACCTGCTGTGGTGGAGTCATACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgcatgtttaatgtatgtcacctgtttatttattaaacttcgtatcttca-aatcgtccactgaaaacaaactgaaacag
||| |||||||| ||||||||||| ||||| ||  ||||  ||||||||||||| |||||  ||||||| || ||||||||||| |||||| |||||||||||||||   | | ||  | || |  | ||||||   ||| ||   | |  | || | |||| | || |||        || |||||
TGAAAGCTTTCCAGCATCTCTCTTAGATCTCCCAACTGTTATGGAGTCATACAAGACATACAATGATTCAGTGCTAATTAAAACAGCTGATATTGGTCAGgtaaaaggataagatttgtttaag--atctgttt---tatcaattgtaggcttttaagaatcttacattgagt-----ttgtaacag

upper sequence: GRMZM2G147614_T01 (Zea mays), 5'ss of exon 3
lower sequence: Vv06s0004g00700.t01 (Vitis vinifera), 5'ss of exon 2
TGAGAGCTTTCCTGCATCTCTCTTGGATCTACCTGCTGTGGTGGAGTCATACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgcatgtttaatgt-atgtcacctgtttatttattaaacttcgtatcttcaaatcgtccactgaaaacaaactgaaacag
 ||    ||||||||||||||  ||||||| |||  |||  |||||||||||||||| ||||| |||  ||| ||||| |||||||||||  ||||||| ||| | |||| ||||| |  | || | || | ||| ||| |  |  |  ||   | |       | | || ||      |  |||||
CGACCATTTTCCTGCATCTCTTCTGGATCTTCCTTGTGTCCTGGAGTCATACAAAACTTATGACGATAGTGTGCTAATCAAAACTGCTGACATTGGTCAAgtaga-gtgc-tgtttgacattatttgacttttttttttttacagttaagtgattgcttcattactaatgcaagttg-ttataacag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|86472598|gb|DY238968.1|DY238968
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAAGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|93013166|gb|EB638686.1|EB638686
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|22543273|gb|BU093711.1|BU093711
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGGAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTTGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|211034641|gb|FL277424.1|FL277424
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGA-GAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|89106294|gb|DY576511.1|DY576511
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|32931457|gb|CF036269.1|CF036269
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCT
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCT
EST: gi|211034640|gb|FL277423.1|FL277423
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|211037077|gb|FL277427.1|FL277427
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|17921703|gb|BM259354.1|BM259354
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|86474214|gb|DY240584.1|DY240584
EST:     AAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGTTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: AAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|32931995|gb|CF036807.1|CF036807
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|74238971|gb|DT646885.1|DT646885
EST:     TACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: TACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|60345606|gb|DN212579.1|DN212579
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|32865677|gb|CF005359.1|CF005359
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|17921875|gb|BM259526.1|BM259526
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|211037075|gb|FL277425.1|FL277425
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGA-GAAAATGATCCTGCTCCAGAARGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|16927590|gb|BM080659.1|BM080659
EST:     ATGACTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|22543221|gb|BU093659.1|BU093659
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTTGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|40334273|gb|CK368343.1|CK368343
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTTGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|9254485|gb|BE344953.1|BE344953
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTTGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|89106285|gb|DY576502.1|DY576502
EST:     CTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: CTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|211527102|gb|FL390752.1|FL390752
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|21331342|gb|BQ486723.1|BQ486723
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTTGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|211037076|gb|FL277426.1|FL277426
EST:     ACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|17921548|gb|BM259199.1|BM259199
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|211240386|gb|FL373755.1|FL373755
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|71435867|gb|DR816917.1|DR816917
EST:     CAGAACATATGATGATTCTGTACTAATTAAAACTGCTGATGGTTGGTCAG                         ATGATTATGGTAAGAGAAGACAATGGTCCTGCTCCCGAAGGAGTCGAGTA
genomic: CAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATG-TTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTA
EST: gi|74235859|gb|DT643773.1|DT643773
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|211183920|gb|FL459369.1|FL459369
EST:     AATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: AATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|74235860|gb|DT643774.1|DT643774
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|211037079|gb|FL277429.1|FL277429
EST:     CATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
genomic: CATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC
EST: gi|78544005|gb|DV621503.1|DV621503
EST:     ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAG                         ATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTTGAGTAC
genomic: ACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 73

TGAGAGCTTTCCTGCATCTCTCTTGGATCTACCTGCTGTGGTGGAGTCATACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTACAAGCATGGGCTTACTCCTCCAATGAGGGATGCACGCAGGCGACGTTTCC


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 58

TGAGAGCTTTCCTGCATCTCTCTTGGATCTACCTGCTGTGGTGGAGTCATACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTACAAGCATGGGCTTACTCCTCCAATGAGGGATGCACGCAGGCGACGTTTCC


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 118

TGAGAGCTTTCCTGCATCTCTCTTGGATCTACCTGCTGTGGTGGAGTCATACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTACAAGCATGGGCTTACTCCTCCAATGAGGGATGCACGCAGGCGACGTTTCC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 160

TGAGAGCTTTCCTGCATCTCTCTTGGATCTACCTGCTGTGGTGGAGTCATACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgc ... actgaaacagATGATTATGGTAAGAGAAGAAAATGATCCTGCTCCAGAAGGAGTCGAGTACAAGCATGGGCTTACTCCTCCAATGAGGGATGCACGCAGGCGACGTTTCC


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TGAGAGCTTTCCTGCATCTCTCTTGGATCTACCTGCTGTGGTGGAGTCATACAAAACATATGATGATTCTGTACTAATTAAAACTGCTGATGTTGGTCAGgtaaaagtgcatgtttaatgtatgtcacctgtttatttattaaacttcgtatcttcaaatcgtccactgaaaacaaactgaaacag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGAT