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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GGAGCGGTTGACTGGTCCGGACCCTGATCTCACGGACCAGGAGAGGCTGATGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttgagaacaactaaccctattcctatgtgctggtgtataaacaaacaagctcattgggatgatcactcaggtggtactgggtactaacaagtatatgcgtatgacatgaactgcag

Basic information

species Zea mays
transcript GRMZM2G052546_T04
intron # 12
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G052546_T04 (Zea mays), 5'ss of exon 12
lower sequence: LOC_Os03g01750.2 (Oryza sativa), 5'ss of exon 14
----------GGAGCGGTTGACTGGTCCGGACCCTGATCTCACGGACCAGGAGAGGCTGATGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttgagaacaactaaccctattcctatg----tgctggtgtataaacaaacaagctcat---tgggatgatcactcaggtggtactgggtactaacaagtata-tgcgtatgacatgaactgcag-----------------------
          |||||| ||||||||||   | || ||||| ||| |  |||| ||||||||||| ||||||||||||||||||||||           ||||| |||  |    |||||| |   | | |  ||| |  |||     ||| || || |     |  | ||| ||   ||     |||| || | | | ||||   |||||   | | | |    ||   || | |  |                         
GAGAATTAAGGGAGCGTTTGACTGGTCAAAATCCCGATCTTACGAAGGAGGAAAGGCTGATGATTAGAGAGTACTTGGAACAGTACGT---------TGAGCGATAGAGg--taagttgaaatttattcagactactagtattgtgttgcaggggttttgctgattatgcttatgcatgaacatatcattcggattggactg--cactaatctgcagaattgacattggattatcgactcgttttgggctcgctgtgcaacag

upper sequence: GRMZM2G052546_T04 (Zea mays), 5'ss of exon 12
lower sequence: LOC_Os03g01750.5 (Oryza sativa), 5'ss of exon 13
----------GGAGCGGTTGACTGGTCCGGACCCTGATCTCACGGACCAGGAGAGGCTGATGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttgagaacaactaaccctattcctatg----tgctggtgtataaacaaacaagctcat---tgggatgatcactcaggtggtactgggtactaacaagtatatgcgtatgacatgaactgcag
          |||||| ||||||||||   | || ||||| ||| |  |||| ||||||||||| ||||||||||||||||||||||           ||||| |||  |    |||||| |   | | |  ||| |  |||     ||| || || |     |  | ||| ||   ||     |||| || | | | ||||   |||||   | |                       
GAGAATTAAGGGAGCGTTTGACTGGTCAAAATCCCGATCTTACGAAGGAGGAAAGGCTGATGATTAGAGAGTACTTGGAACAGTACGT---------TGAGCGATAGAGg--taagttgaaatttattcagactactagtattgtgttgcaggggttttgctgattatgcttatgcatgaacatatcattcggattggactg--cactaatctgcag----------------------
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|76914763|gb|DV165676.1|DV165676
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGG                         T
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGA-Ggtccagttga ... tgaactgcag-
EST: gi|76914763|gb|DV165676.1|DV165676
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGG                         T
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGA-Ggtccagttga ... tgaactgcag-
EST: gi|76914763|gb|DV165676.1|DV165676
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGG                         T
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGA-Ggtccagttga ... tgaactgcag-
EST: gi|76914763|gb|DV165676.1|DV165676
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGG                         T
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGA-Ggtccagttga ... tgaactgcag-
EST: gi|76914763|gb|DV165676.1|DV165676
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGG                         T
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGA-Ggtccagttga ... tgaactgcag-
EST: gi|45847997|gb|CN071940.1|CN071940
EST:     TGATCAGAGAGTACCTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAG                         AAGAATGATGATGACTGAGGAAGAGAAGAGACACACCATGAGACGACGGAC
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcagAAGAATGATGATGACTGAGGAAGAGAAGAGACACACCATGAGACGACGGAC
EST: gi|211409237|gb|FL441028.1|FL441028
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAG                         AAGAATGATGATGACTGAGGAAGAGAAGAGACACACCATGAGACGACGGAC
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcagAAGAATGATGATGACTGAGGAAGAGAAGAGACACACCATGAGACGACGGAC
EST: gi|33091895|gb|CF051889.1|CF051889
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAG                         AAGAATGATGATGACTGA
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcagAAGAATGATGATGACTGA
EST: gi|78021110|gb|DV489497.1|DV489497
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGG                         T
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGA-Ggtccagttga ... tgaactgcag-
EST: gi|78021110|gb|DV489497.1|DV489497
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGG                         T
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGA-Ggtccagttga ... tgaactgcag-
EST: gi|78021110|gb|DV489497.1|DV489497
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGG                         T
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGA-Ggtccagttga ... tgaactgcag-
EST: gi|78021110|gb|DV489497.1|DV489497
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGG                         T
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGA-Ggtccagttga ... tgaactgcag-
EST: gi|78021110|gb|DV489497.1|DV489497
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGG                         T
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGA-Ggtccagttga ... tgaactgcag-
EST: gi|15426921|gb|BI542743.1|BI542743
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAG                         AAGAATGATGATGACTGAGGAAGAGAAGAGACACACCATGAGACGACGGAC
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcagAAGAATGATGATGACTGAGGAAGAGAAGAGACACACCATGAGACGACGGAC
EST: gi|5650248|gb|AI920608.1|AI920608
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAG                         G
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcag-
EST: gi|5650248|gb|AI920608.1|AI920608
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAG                         G
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcag-
EST: gi|5650248|gb|AI920608.1|AI920608
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAG                         G
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcag-
EST: gi|5650248|gb|AI920608.1|AI920608
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAG                         G
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcag-
EST: gi|5650248|gb|AI920608.1|AI920608
EST:     TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAG                         G
genomic: TGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcag-

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 2 RNA-Seq libraries.


Block sizes: 40 (upstream exon), 10 (downstream exon)
Score: 1

GGAGCGGTTGACTGGTCCGGACCCTGATCTCACGGACCAGGAGAGGCTGATGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcagAAGAATGATGATGACTGAGGAAGAGAAGAGACACACCATGAGACGACGGACCGAAGCCGACCTGACCGCACCTTCCTTTACGGAGAGGTCGGGCTTCCCC


Block sizes: 32 (upstream exon), 18 (downstream exon)
Score: 1

GGAGCGGTTGACTGGTCCGGACCCTGATCTCACGGACCAGGAGAGGCTGATGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttga ... tgaactgcagAAGAATGATGATGACTGAGGAAGAGAAGAGACACACCATGAGACGACGGACCGAAGCCGACCTGACCGCACCTTCCTTTACGGAGAGGTCGGGCTTCCCC




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Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GGAGCGGTTGACTGGTCCGGACCCTGATCTCACGGACCAGGAGAGGCTGATGATCAGAGAGTACTTGGAACAGTACGCGGATGCCGCCGAGCGCTAGGAGgtccagttgagaacaactaaccctattcctatgtgctggtgtataaacaaacaagctcattgggatgatcactcaggtggtactgggtactaacaagtatatgcgtatgacatgaactgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -TGCCGCCG