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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gttgttgtgatctattaccttattctatctttgctatacacattgtatatctcaggttacagttgtatttttattgaaatatgggaaacaactttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA

Basic information

species Zea mays
transcript GRMZM2G148323_T01
intron # 9
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G148323_T01 (Zea mays), 3'ss of exon 9
lower sequence: LOC_Os10g33680.1 (Oryza sativa), 3'ss of exon 9
gttgt-tgtgatctattaccttattctatctttgctatacacattgtatatctca---ggttacagttgtatttttattgaaatatgggaaacaactttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA
 |||  | |    | || |||||   |  ||||  |||    | | ||| |  ||     |||   |  | | || ||||||  ||  ||| ||| |||  |||||||||||| || || |||||||| ||||| ||||||||   || |||||||||||||||||| ||||||||||||||||| ||||||||||||||||||
-ttgcctattgagttttgccttacagtggctttaatat-tgtagtatattttacaactaattatgttactgtattcattgaa-catatgaaccaatttt-acagGTTTAAGTGTATTGACCTTGATGGAAATGGCATTTTAACCACCACTGAAATGCAATTTTTCTATGAGGAGCAATTGCACCGGATGGAGTGCATGGCACAA

upper sequence: GRMZM2G148323_T01 (Zea mays), 3'ss of exon 9
lower sequence: GLYMA03G41180.1 (Glycine max), 3'ss of exon 9
gttgttgtgatctattaccttattctatctttgctatacacattgtatatctcaggtt-acagttgtatttttattgaaatatgggaaacaactt-tcgc----agGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA
     |      |    ||| | |  |   ||       |||||| |  | | |  ||    ||||  | ||  | |  || || ||    |  | ||      ||||| |||||||| ||| | ||||| |||||  || | ||    |||||| |||||||||| || |||||||||||||| ||||||||||||||||| |||
-----tccaccttgcctcctaagtacactcttag-gcgaacattggaagtttgacctttgtggttgactcttgttagctatttgtgatttgatgtgtcatgattagGTTCAAGTGCATTGATTTGGATGGAAATGGAGTTCTGACACGGAATGAACTGCAATTTTTTTATGAGGAGCAATTGCATCGAATGGAGTGCATGGCCCAA

upper sequence: GRMZM2G148323_T01 (Zea mays), 3'ss of exon 9
lower sequence: GLYMA19G43790.1 (Glycine max), 3'ss of exon 9
gttgttgtgatctattaccttattctatctttgctatacacattgtatatctcaggttacagttgtatttttattgaaatatgg---gaaacaactttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA
  | ||      |    ||| | |  |   ||       |||||| |  | | |  ||   | |  | | |||   |  | ||    ||     | |    ||||| |||||||| ||| | ||||| |||||  || | ||    |||||| |||||||||| || |||||||||||||| ||||||||||||||||| |||
--ttttccaccttgcctcctaagtacactcttag-gcgaacattggaagtttgatctttgtggttgactattagctatttgtgatttgacgtgtcatgattagGTTCAAGTGCATTGATTTGGATGGAAATGGAGTTCTGACACGGAATGAACTGCAATTTTTTTATGAGGAGCAATTGCATCGAATGGAGTGCATGGCCCAA

upper sequence: GRMZM2G148323_T01 (Zea mays), 3'ss of exon 9
lower sequence: GLYMA10G32500.1 (Glycine max), 3'ss of exon 9
gttgttgtgatctattaccttattctatctttgcta--tacacattgtatatctcaggttacagttgtatttttattgaaatatgggaaacaactttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA
 |  || |    | | |  | | | | ||  |   |  | || | | |   |  | |  |  | | | |  |  |   |  | |    || || | | | ||||| || ||||| ||| | ||||| |||||  |  | ||    ||||||||||| || || || |||||||| ||||| || |||||||||||||| |||
atatttctagattgtaaattcagtatttcagtagcaagttcagaatttgatttgctgcatttaatagaaactcaac--atttgtttttaaaaattcttgtagGTTCAAATGCATTGATTTGGATGGAAATGGAGTGCTGACACCAAATGAAATGCAGTTCTTTTATGAGGAGCAGTTGCATCGGATGGAGTGCATGGCCCAA

upper sequence: GRMZM2G148323_T01 (Zea mays), 3'ss of exon 9
lower sequence: GLYMA20G35090.1 (Glycine max), 3'ss of exon 9
-gttgttgtgatctattaccttattctatctttgctatacacattgtatatctcaggttacagttg-tatttttattgaaatatgggaaacaactttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA
  |  || |    | | |  | |   ||| || |    | |    | || |     | | || ||  ||          | | |   ||| || | | | ||||| || ||||| ||| | ||||| |||||  |  | ||    ||||||||||| || || || |||||||| ||||| || |||||||||||||| |||
tatatttatagattgtaaattcag--tattttggtagcaaattcagaatttgatttgctgcatttaatagaaactcaacattttcaaaaaaaaatcttgtagGTTCAAATGCATTGATTTGGATGGAAATGGAGTGCTGACACCTAATGAAATGCAGTTCTTTTATGAGGAGCAGTTGCATCGGATGGAGTGCATGGCCCAA

upper sequence: GRMZM2G148323_T01 (Zea mays), 3'ss of exon 9
lower sequence: Vv07s0005g03730.t01 (Vitis vinifera), 3'ss of exon 10
---gttgttgtgatctattaccttattctatctttgctatacacattgtatatctcaggttacagttgtatttttattgaaatatgggaaacaactttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA
   |   ||  | | |    |  ||   ||  |||| |   |  | | ||| | | | || |    |||   |   |     | ||       | |||| |||||| |||||||| ||| | ||||| |||||  |  | ||  | ||||||||||| || || || |||||||||||||| |||||||| |||||||| |||
tatgggatta-ggtttgaggcagtagcttaa-tttgatgctctaaatttatttgttatgtaatcaatgtgcatcactcactttgtgaatttttattttc-cagGTTCAAGTGCATAGATTTGGATGGAAATGGAGTGCTTACACTGAATGAAATGCAGTTCTTTTATGAGGAGCAATTGCATCGAATGGAATGCATGGCCCAA

upper sequence: GRMZM2G148323_T01 (Zea mays), 3'ss of exon 9
lower sequence: PP1S7_163V6.1 (Physcomitrella patens), 3'ss of exon 9
-----------------------------------------------------------------------------------------gttgttgtgat-ctattaccttattctatctttgctatacacattgta-tatctcaggttacagttgtatttttattgaaatatgggaaacaactttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA
                                                                                          |  |||| |  |    |    |||  || |  |  |   | |||  | ||     |   ||| ||   ||  |  |   ||   ||| ||   |   ||||||| |||||  | || || ||| || | || ||  | |   | ||||| ||||||| ||| |||||||||||| | || || |||||||||||||| || 
gtaagtgccttgacatggtctctaaaacattttctgcacctccatatcttaactgtgaatttacatgaatacaaactgtctgctttctgataattgtaacgcggacaatacattagat-tccgtcacgtatattacaataaaatcgaggacattttgtttaatgctttcatcctggacacg--tgatgcagGTTCAAGTGTGTAGACCTGGATTGTGACGGGATGATTATACTAAATGAGATGCAATATTTTTACGAGGAGCAACTTCATCGGATGGAGTGCATGGCTCAG

upper sequence: GRMZM2G148323_T01 (Zea mays), 3'ss of exon 9
lower sequence: PP1S191_94V6.1 (Physcomitrella patens), 3'ss of exon 10
----------------------------------------------------------------------gttgttgtgatctattaccttattctatctttgc-tatacacattgt-atatctcaggttacagttgtatttttattgaaatatgggaaacaactttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA
                                                                      || ||| |    | ||||    ||  ||  ||    ||   |||||| |||| ||    |  | |  |  | | |   || |   | ||| ||  ||   ||||| |||||  | ||| | ||| || ||||  |  | |||   ||||||||||| | ||| || ||||||||||| || |||||||| || |||||||||
gtaatttttttgtttttttcttattctttttaattttatttattaatttatttattttattatctcccatgtagttatttctttttacaagcttgaattcttaaacatgatcattgtgatatatctatatttaatgattatctaac--aagtggtgaaaataaaatt---agGTTCAAGTGTGTGGATTTAGATTGTGATGGGGTGATCACTGCAAATGAAATGCATTATTTTTATGAGGAGCAATTACATCGAATGGAATGTATGGCACAA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|87156079|gb|DY400868.1|DY400868
EST:     TTTATATTATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATACTG                         GTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAAT
genomic: TTTATATTATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATATTGgtaagtggta ... actttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAAT
EST: gi|23199745|gb|BU582310.1|BU582310
EST:     TTTATATTATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATACTG                         GTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAAT
genomic: TTTATATTATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATATTGgtaagtggta ... actttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAAT
EST: gi|211505069|gb|FK974737.1|FK974737
EST:     TTTATATTATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATACTG                         GTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAAT
genomic: TTTATATTATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATATTGgtaagtggta ... actttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAAT
EST: gi|26560172|gb|CA832407.1|CA832407
EST:     TTATATTAATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATACTG                         GTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAAT
genomic: TTATATT-ATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATATTGgtaagtggta ... actttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 122

AATTCACAAGCATGACAGAAGGAAAGATGGGTTATGAAGACTTTGTTTACTTTATATTATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATATTGgtaagtggta ... actttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 78

AATTCACAAGCATGACAGAAGGAAAGATGGGTTATGAAGACTTTGTTTACTTTATATTATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATATTGgtaagtggta ... actttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 93

AATTCACAAGCATGACAGAAGGAAAGATGGGTTATGAAGACTTTGTTTACTTTATATTATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATATTGgtaagtggta ... actttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 84

AATTCACAAGCATGACAGAAGGAAAGATGGGTTATGAAGACTTTGTTTACTTTATATTATCAGAGGAAGACAAATCATCTGAGCCTAGTCTTGAATATTGgtaagtggta ... actttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tatatctcaggttacagttgtatttttattgaaatatgggaaacaactttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA
                                              ctttcgc  CT-rich tract
               agttgtatttttattg  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gttgttgtgatctattaccttattctatctttgctatacacattgtatatctcaggttacagttgtatttttattgaaatatgggaaacaactttcgcagGTTTAAGTGCATCGATCTTGATGGTAATGGTATTTTAACTTTCAATGAAATGCAATTTTTCTACGAGGAGCAATTGCACCGAATGGAGTGCATGGCACAA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGCA
-ttgttgt
- - - - - - - - - - - - - - - - - -atacaca