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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtcggtattcactatctattgaagtgacatttagcctttatttagctgggtgcttgcttaattcatgtccttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT

Basic information

species Zea mays
transcript GRMZM2G103805_T02
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G103805_T02 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os06g45110.1 (Oryza sativa), 3'ss of exon 8
gtcggtattcact----atctattgaagtgacatttagcctttatttagctgggtgcttgcttaattcatgtcctt------ggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT
||| ||||| | |    || ||||||||||||||||     ||  || |  |  | |||||| ||| ||| || ||        ||||||||||||||||||||||| ||  |||||||||| ||||||||||| |||||||||||||||||||||||||| ||||||||||| ||||  ||||||||||
gtcagtatttatttacaatatattgaagtgacatttgattctt--ttgggggcttacttgctaaatacatatcattttgcatatcttcagATGGTAGATCCTGGTGGAATTGCTAATTGGTCAGTTACACATGTTGATTGGTCTGAGGGAAAATGGCATCCGAGGTCATATAGAGCTGCAGATGTCACCT

upper sequence: GRMZM2G103805_T02 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA14G17110.1 (Glycine max), 3'ss of exon 8
gtcggt------attcactatctattgaagtgacat--ttagcctttatttagctgggtgcttgcttaattcatgtccttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT
||| ||      ||    | ||| || || ||  ||  |  || | ||| |  || |  |||  || | ||  |||    |  || ||||| | ||||||||||| ||| | ||||||||  | ||| |||| ||||||||||| ||||||||||||||||||||||  || |||   ||| | || |
gtcagtgcaccaatctgttctcttttaaattgcaatgataggcatctatctcactcgcagctaactcatttggtgt----gtatttagATGCTTGATCCTGGTGGAATCGCAAATTGGTCGATAACATATGTTGATTGGTCTGAAGGAAAATGGCATCCAAGGTCATTCAGAGCTCGGGATATTACAT

upper sequence: GRMZM2G103805_T02 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA17G29670.1 (Glycine max), 3'ss of exon 8
gtcggt------attcactatctattgaagtgacat--ttagcctttatttagctgggtgcttgcttaattcatgtccttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT
||| ||      ||    | ||| || || ||  ||  |  || ||||| || ||    |||  ||   ||  |||    |  || ||||| | ||||||||||| ||| | |||||||| || ||| |||| ||||||||||| ||||||||||||||||| ||||  || |||   ||| | || |
gtcagtccaccaatctgttctcttttaaattgcaatgataggcatttatctatctcacagctaactcgttttgtgt----gtatttagATGCTTGATCCTGGTGGAATCGCAAATTGGTCGGTAACATATGTTGATTGGTCTGAAGGAAAATGGCATCCAAGATCATTCAGAGCTCGGGATATTACTT

upper sequence: GRMZM2G103805_T02 (Zea mays), 3'ss of exon 8
lower sequence: AT5G57270.2 (Arabidopsis thaliana), 3'ss of exon 8
-------gtcggtattcactatctattgaagtgacatttagcctttatttagctgggtgcttgcttaattcatgtccttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT
       |    ||   ||| ||| |       |   ||   || |  ||  || |   |||  | || ||   |  ||   |  ||||| | |||||||| || ||||| || ||||| || ||| |||| |||||||||||| | | ||||||||||| | ||||||| ||    |||||| |  
gtgaggagattataacaactttctctcttttaaaagctttcactgtgatt--cttgtcccttaatgaaatct--ttgttccttaaagATGCTTGATCCTGGAGGAATCTCGAACTGGTCAGTCACATATGTTGATTGGTCTGAGCGTAGATGGCATCCAAAGACATATAGAGCACGCGATGTCTCAC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|6020571|gb|AW065604.1|AW065604
EST:     AAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAAT                         ATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
genomic: AAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAATgtcggtattc ... ttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
EST: gi|76916148|gb|DV166259.1|DV166259
EST:     AAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAAT                         ATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
genomic: AAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAATgtcggtattc ... ttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
EST: gi|78022101|gb|DV490488.1|DV490488
EST:     AAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAAT                         ATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
genomic: AAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAATgtcggtattc ... ttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
EST: gi|78089620|gb|DV517994.1|DV517994
EST:     AAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAAT                         ATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
genomic: AAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAATgtcggtattc ... ttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
EST: gi|83279045|gb|DV943053.1|DV943053
EST:     TATTTGCCAACCCTGTTCAAT                         ATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
genomic: TATTTGCCAACCCTGTTCAATgtcggtattc ... ttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
EST: gi|4730457|gb|AI649623.1|AI649623
EST:     TATTTGCCAACCCTGTTCAAT                         ATGGTAGATCCTGGNGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG
genomic: TATTTGCCAACCCTGTTCAATgtcggtattc ... ttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 43 (downstream exon)
Score: 176

CCAGCAGAAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAATgtcggtattc ... ttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 495

CCAGCAGAAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAATgtcggtattc ... ttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 626

CCAGCAGAAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAATgtcggtattc ... ttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 144

CCAGCAGAAGGGCGCAACTGTATTGCAGATGAGCATTATTTGCCAACCCTGTTCAATgtcggtattc ... ttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gacatttagcctttatttagctgggtgcttgcttaattcatgtccttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT
                              gcttaat  putative branch site (score: 144)
                                        tgtcctt  putative PPT
           tttattta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtcggtattcactatctattgaagtgacatttagcctttatttagctgggtgcttgcttaattcatgtccttggcttcagATGGTAGATCCTGGTGGGATCTCTAATTGGTCTGTTACACATGTGGATTGGTCTGAGGGAAAATGGCATCCAAGGTCATATAGCGCTGATGATGTCACCT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGAT