ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

...aaattatcagacggaaattttgtatgttgatgttatgtatagttctgatttgtaagttactgtgatgtggaattgatgccgtttaatatttgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGATTGTTGGTGGATCAACTTTTGGAAATGTAGCTATTGCAGATCAAACCTCA

Basic information

species	Zea mays
transcript	GRMZM2G087105_T01
intron #	6
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G087105_T02 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os11g07970.1 (Oryza sativa), 3'ss of exon 6

aaattatcagacggaaattttgtatgttgatgttatgtatagttctgatttgtaagttactgtgatgtggaattgatgccgtttaatatttgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGATTGTTGGTGGATCAACTTTTGGAAATGTAGCTATTGCAGATCAAACCTCA
||   |  | |    |      | ||    ||| ||||| |||||||  || ||  ||| | ||   || |  |||||||  || |  | |||  |||||||||||||| |||||  ||||||| ||||  ||||| ||||||||||| |||| || ||| || || |||||||||||||||||||||||||||||||| 
aatgcagaaaaaaagagacaattttgcccctgtgatgtacagttctgcattctatattagtatgtcctgcagctgatgcctatttaactgtgttatacagGCTTCACTCTTCTCCCTTGACATGCTTGACAATAACAGATGACCAGCTCATTGCTGCTGGTTCTACATTTGGAAATGTAGCTATTGCAGATCAAACCTCT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|78107838|gb|DV526256.1|DV526256

EST:     CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCG                         GCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT
genomic: CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT

EST: gi|211348374|gb|FL045514.1|FL045514

EST:     CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCG                         GCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT
genomic: CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT

EST: gi|76021370|gb|DT948540.1|DT948540

EST:     CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCG                         GCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT
genomic: CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT

EST: gi|74238280|gb|DT646194.1|DT646194

EST:     CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCG                         GCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT
genomic: CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT

EST: gi|211348372|gb|FL045512.1|FL045512

EST:     CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCG                         GCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT
genomic: CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT

EST: gi|6021302|gb|AW066230.1|AW066230

EST:     CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCG                         GCTTCACTCCTCTCCTGNGACATGTTTGATGATAACGGATGACCAGCTGAT
genomic: CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT

EST: gi|76011998|gb|DT939168.1|DT939168

EST:     CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCG                         GCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT
genomic: CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT

EST: gi|7216529|gb|AW562651.1|AW562651

EST:     GTTGCTCACGTATTTATCG                         GCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT
genomic: GTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT

EST: gi|148986029|gb|EE029876.2|EE029876

EST:     CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCG                         GCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT
genomic: CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT

EST: gi|148991227|gb|EE032692.2|EE032692

EST:     CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCG                         GCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT
genomic: CGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGAT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 164

TTTTGCTGATCCGGAGGTTGTAATTGGTTGTGAGGATGGTCGAGCCTTTGTATATGATATGTACAGCAGGAGTTGCTCACGTATTTATCGgtaagtatta ... tgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGATTGTTGGTGGATCAACTTTTGGAAATGTAGCTATTGCAGATCAAACCTCA

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 107

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 70

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 122

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tgatttgtaagttactgtgatgtggaattgatgccgtttaatatttgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGATTGTTGGTGGATCAACTTTTGGAAATGTAGCTATTGCAGATCAAACCTCA

                                    tttaatatttgtacta  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
aaattatcagacggaaattttgtatgttgatgttatgtatagttctgatttgtaagttactgtgatgtggaattgatgccgtttaatatttgtactacagGCTTCACTCCTCTCCTGTGACATGTTTGATGATAACGGATGACCAGCTGATTGTTGGTGGATCAACTTTTGGAAATGTAGCTATTGCAGATCAAACCTCA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGTGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGATCA
- - - - - - - - - - - - tgttgat
- - - - - - - - - - - - - - - - -tatgtat
- - - - - - - - - - - - - - - - - - - - - - -tgatttg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tgtgatg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgatgcc