ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

...ggattcatcggctcactacatctaatttactatttgtggacatatgcttatgggatgaaaaactgactgaatatctgctgaaaaacttgctataatttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGGCATGCGTCGAGATTGGCATGTTCGTGACGAAAACTCTGGACGCACAATC

Basic information

species	Zea mays
transcript	GRMZM5G829544_T01
intron #	3
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM5G829544_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os11g43820.1 (Oryza sativa), 3'ss of exon 3

--ggattcatcggctcactacatctaatttactatttgtggacatatgcttatgggatgaaaaactgactgaatatctgctgaaaaacttgctataattt-agGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGGCATGCGTCGAGATTGGCATGTTCGTGACGAAAACTCTGGACGCACAATC
     ||||     |   |  |  | |  ||| ||| |  ||| | |   | |||    ||||| |||| ||     ||   |  ||| ||||||  ||| ||||||||||| |||||||| ||||||||||| ||||||||||| |||||||||||||||||||| ||||||||||||||| | |||||||| || ||||||
gcaatttcaaattgtttttttagttcagatacaatt-gccgacgtctttctgtggatcaaaaaaatgacagatgtattgt--accaacctgctatggttttagGGGAGATACGGTTCAAGTTGACACATGGGTTGCTGCTGCTGGCAAAAATGGCATGCGTCGAGACTGGCATGTTCGTGACTACAACTCTGGCCGAACAATC

upper sequence: GRMZM5G829544_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os02g43090.4 (Oryza sativa), 3'ss of exon 4

-ggattcatcggctcactacatctaatttactatttgtggacatatgcttatgggatgaaaaactgactgaatatctgctgaaaaacttgctataattt-agGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGGCATGCGTCGAGATTGGCATGTTCGTGACGAAAACTCTGGACGCACAATC
    ||||     |   |  |  | |  ||| ||| |  ||  | |   | |||    ||||         || | |  | |  ||| ||||||  ||| ||||||||||  |||||||| ||||||||||| ||||||||||| |||||||||||||||||||| ||||||||||||||| | |||||||| || ||||||
caatttcaaactgtttttttagttcagatacaatt-gccgatgtctttctgtggatcaaaaagaatgacagatgtattgt-accaacctgctatggttttagGGGAGATATGGTTCAAGTTGACACATGGGTCGCTGCTGCTGGCAAAAATGGCATGCGTCGAGACTGGCATGTTCGTGACTACAACTCTGGCCGAACAATC

upper sequence: GRMZM5G829544_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os06g05130.1 (Oryza sativa), 3'ss of exon 3

-----ggattcatcggctcactacatctaatttactatttgtggacatatgcttatgggatgaaaaactgactgaatatctgctgaaaaacttgctataa---tttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGGCATGCGTCGAGATTGGCATGTTCGTGACGAAAACTCTGGACGCACAATC
        || ||  |   |    |     | | || ||| || |  | ||  | | |  |  | || |        ||  |||||||   | | |  ||   |||||||||||||  || ||||| ||||||||||||||||||||||| |||||||||||||||||||||||||||||||| ||| | |||||||| |  ||||||
attttaagtttatgag---aaggaaaacggtctgctgtttttgaat-tctgaatgtagtttacacaatttctc----ataggctgaaattttggttgcaaatttttagGGGAGATATGGTCCAAGTTGACACATGGGTAGCTGCTGCTGGCAAAAATGGCATGCGTCGAGATTGGCATGTTCGGGACTACAACTCTGGTCAAACAATC

upper sequence: GRMZM5G829544_T01 (Zea mays), 3'ss of exon 3
lower sequence: Vv11s0016g04480.t01 (Vitis vinifera), 3'ss of exon 3

ggattcatcggctcactacatctaatttactatttgtggacatatgcttatgggatgaaaaactgactgaat---atctgctgaaaaacttgctataatttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGGCATGCGTCGAGATTGGCATGTTCGTGACGAAAACTCTGGACGCACAATC
    | ||| |   |   | |  || | | ||||  |  || | |  |       | | |||||||  |||    ||    ||||    || | ||  |  ||||| |||  |||||||||||| || ||||||  |||  ||||||| |||||||||||||| ||||||    | |||||    ||  ||||    ||  | 
-acattatctgtagaaagc-tacaaatgattattcataaacttct-ttggaatatttagaaactgatagaaattgatggattgaactgatttccatcttgcagGGGTGATGTTGTTCAAGTGGATACTTGGGTATGTGCATCTGGGAAGAATGGCATGCGTCGTGATTGGATAATCCGTGATTGCAAAACTGGGGAAACTCTA

upper sequence: GRMZM5G829544_T01 (Zea mays), 3'ss of exon 3
lower sequence: PP1S121_22V6.1 (Physcomitrella patens), 3'ss of exon 2

-------------------------------------------------ggattcatcggctcactacatctaatttactatttg-tggacatatg-cttatgg---------gatgaaaaactgact-----gaatatctgctgaaaaacttg-ctataatt-----tagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGGCATGCGTCGAGATTGGCATGTTCGTGACGAAAACTCTGGACGCACAATC
                                                  || ||  | | |   |  |   | ||||| |   | ||||    || |||| ||         | |  | ||| | ||     ||   | ||    | | | || | ||   |      |||||| ||| |||| |  ||||||||||||| || ||| ||||||| ||||| |||||||| ||||||| ||| || ||| | || || ||||  |   | 
gtacgttgcacatagattagaattccctcttcgatgtgctaattggcagagagtcgcccggtatatgaacggagtttaccacgaggtggagggttgtcttacggcttctttttgttacagaaccggcttccaagagcttgtgacatagatcatgtccatctgtgtgagcagGGGAAATATTGTTGAGATGGACACATGGGTGGCAGCTTCTGGGAAGAATGGAATGCGTCGGGATTGGCTTGTGCGAGACTACAAATCAGGACAGATTTTA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|148991050|gb|EE032494.2|EE032494

EST:     ATCTGGGTTGTCAGCAAAATTCAGCTTCTTGTTGAGCAATACCCCTCATG                         GGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGG
genomic: ATCTGGGTTGTCAGCAAAATTCAGCTTCTTGTTGAGCAATACCCCTCATGgtacgtttct ... tataatttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGG

EST: gi|76019085|gb|DT946255.1|DT946255

EST:     ATCTGGGTTGTCAGCAAAATTCAGCTTCTTGTTGAGCAATACCCCTCATG                         GGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGG
genomic: ATCTGGGTTGTCAGCAAAATTCAGCTTCTTGTTGAGCAATACCCCTCATGgtacgtttct ... tataatttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGG

EST: gi|6137960|gb|AW134414.1|AW134414

EST:     ATCTGGGTTGTCAGCAAAATTCAGCTTCTTGTTGAGCAATACCCCTCATG                         GGGAGATACTGNTCAAGNGGACACATGGGTAGCTGCTGCTGGGAAAAATGG
genomic: ATCTGGGTTGTCAGCAAAATTCAGCTTCTTGTTGAGCAATACCCCTCATGgtacgtttct ... tataatttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGG

EST: gi|211372077|gb|FL076465.1|FL076465

EST:     TCTGGGTTGTNAGCAAAATTSSAGCTTCTTGTTGAGCAATACCCCTCATG                         GGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAA
genomic: TCTGGGTTGTCAGCAAAATTC-AGCTTCTTGTTGAGCAATACCCCTCATGgtacgtttct ... tataatttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAA

EST: gi|78179128|gb|DV549501.1|DV549501

EST:     ATCTGGGTTGTCAGCAAAATTCAGCTTCTTGTTGAGCAATACCCCTCATG                         GGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGG
genomic: ATCTGGGTTGTCAGCAAAATTCAGCTTCTTGTTGAGCAATACCCCTCATGgtacgtttct ... tataatttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 128

TTCTTGGAGATGGTTTTGGCGCCACACCAGAGATGAGCAAACGAAACTTGATCTGGGTTGTCAGCAAAATTCAGCTTCTTGTTGAGCAATACCCCTCATGgtacgtttct ... tataatttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGGCATGCGTCGAGATTGGCATGTTCGTGACGAAAACTCTGGACGCACAATC

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 38

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 37

Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 75

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

gcttatgggatgaaaaactgactgaatatctgctgaaaaacttgctataatttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGGCATGCGTCGAGATTGGCATGTTCGTGACGAAAACTCTGGACGCACAATC

               aactgac  putative branch site (score: 75)
                                             tataattta  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ggattcatcggctcactacatctaatttactatttgtggacatatgcttatgggatgaaaaactgactgaatatctgctgaaaaacttgctataatttagGGGAGATACTGTTCAAGTGGACACATGGGTAGCTGCTGCTGGGAAAAATGGCATGCGTCGAGATTGGCATGTTCGTGACGAAAACTCTGGACGCACAATC

- - - - - - - -ctacatc
- - - - - - - - - - - - - - - - - - - - - -atgctta
- - - - - - - - - - - - - - - - - - - - - - - - - - - atgaaaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ctgactg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tctgctg