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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...agactatattccacaaaaaatgttttcgggcccaactgaatcatacccctgcttgctgccccttttgttctgtgacacattctgcgtatcctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCACTTCTGGTCGG

Basic information

species Zea mays
transcript GRMZM2G082664_T01
intron # 1
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211015453|gb|FL446495.1|FL446495
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|71433814|gb|DR814864.1|DR814864
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTCGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|211035970|gb|FL104470.1|FL104470
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|76924992|gb|DV170232.1|DV170232
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|148945041|gb|EC890225.2|EC890225
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTCGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|149003162|gb|EE038900.2|EE038900
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTCGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|211035971|gb|FL104471.1|FL104471
EST:     CGCCGGATCCTCTCTCAAGCTCGAGCGGCCTTGGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CGCCGGATCCTCTCTCA-GCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|211038407|gb|FL104474.1|FL104474
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|149028361|gb|EE286119.2|EE286119
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTCGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|88758154|gb|DY542295.1|DY542295
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTCGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|211038409|gb|FL104476.1|FL104476
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCT
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCT
EST: gi|211038406|gb|FL104473.1|FL104473
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|116827363|gb|EC871258.1|EC871258
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGTGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
EST: gi|211038408|gb|FL104475.1|FL104475
EST:     CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCG                         GGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA
genomic: CCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 34 (upstream exon), 49 (downstream exon)
Score: 56

GCTGCCGAAGCCGAACCCTTCTCTCGCCCGGCCTGCCGTAGTCCTCCACTCCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCACTTCTGGTCGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 75

GCTGCCGAAGCCGAACCCTTCTCTCGCCCGGCCTGCCGTAGTCCTCCACTCCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCACTTCTGGTCGG


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 38

GCTGCCGAAGCCGAACCCTTCTCTCGCCCGGCCTGCCGTAGTCCTCCACTCCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCACTTCTGGTCGG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 103

GCTGCCGAAGCCGAACCCTTCTCTCGCCCGGCCTGCCGTAGTCCTCCACTCCGCCGGATCCTCTCTCAGCTCGAGCGGTCTTGGAGATCCGTCCGTCCCGgtgagtagcg ... ctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCACTTCTGGTCGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cccctgcttgctgccccttttgttctgtgacacattctgcgtatcctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCACTTCTGGTCGG
                 ttttgtt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
agactatattccacaaaaaatgttttcgggcccaactgaatcatacccctgcttgctgccccttttgttctgtgacacattctgcgtatcctggtaccagGGATCATTTGGCGCGGAGGTTTGGCCTGGGAAAATGGCAAACATACGTGCACTTCTGGTCGG

- - - - - - - aaaaaat
- - - - - - - - - - - - - - - - - -ctgaatc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttctgtg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - attctgc