ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtaaccatgtcatctgaatccatttccccttcagatatatcacatgtactctcttcctaaatagacttggttctggtatgacagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCCCTTGGGGAGTTCATTCTGACTCATCCAAACATCAAG

Basic information

species	Oryza sativa
transcript	LOC_Os05g36270.1
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os05g36270.3 (Oryza sativa), 3'ss of exon 5
lower sequence: GRMZM2G322953_T03 (Zea mays), 3'ss of exon 4

gtaaccatgtcat-ctgaatccattt------------ccccttcagatatatcacatgtactctcttcctaa----atagact--tggttctgg-tatgacagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCCCTTGGGGAGTTCATTCTGACTCATCCAAACATCAAG
||||| || |  | ||  ||||| ||            |||   | |  |  |||| |  ||||   |  |||    || ||||   | | |    |||| ||||||||| |||||||||| || ||||| || || ||||| || |||||||||||||| ||||||||| ||||||||||||| ||||||
gtaactatctattgctagatccactttagtttttacgccccggccggccaattcacttcgactcgtcttataactgtatcgactgccgttaccaactatggcagCTTGTGCTGAGCACTGGGAACGGAGTCAATGGCTTCACTCTCGATCCTTCCCTTGGAGAGTTCATTTTGACTCATCCAAATATCAAG

upper sequence: LOC_Os05g36270.3 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA16G28310.1 (Glycine max), 3'ss of exon 7

-gtaaccatgtcatctga---atccatttccccttcagat-atatcacatgtactct-cttcctaaatagacttggttctggtatg----------acagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCCCTTGGGGAGTTCATTCTGACTCATCCAAACATCAAG
  ||||  | |   |||    |||||    |  || |  | || | | |  |||  | |||  | | | || |    | |||||||           ||||||||||| |||||||||   || || || |||||||||||||| || || ||||| |||||||| || ||||| || ||||| |||
aataacattttttcctggtatatccaaggactgttgacttcattttaaacttacaatgcttaattactggattacaatttggtatgtaatggatttgcagCTTGTGTTAAGCACTGGAGCAGGTGTTAATGGTTTCACCCTTGACCCATCTCTTGGTGAGTTCATCCTAACTCACCCTAACATTAAG

upper sequence: LOC_Os05g36270.3 (Oryza sativa), 3'ss of exon 5
lower sequence: Vv18s0072g00770.t01 (Vitis vinifera), 3'ss of exon 6

---------------gtaaccatgtcatctgaatccatttccccttcaga-tatatcacatgtac-tctcttcctaaatagacttggttctggtatgacagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCCCTTGGGGAGTTCATTCTGACTCATCCAAACATCAAG
                  | | | |  ||||      | |    ||   | |  ||    | ||  | |   | ||| |   | |    ||  | || ||||||||||| |||||||||| ||| || || || || ||||||||||| || |||||||||||||| || ||||| |||||||| |||
tctgcagtctagacttccatctttttctctggtgttgtattaatttggtactgcatttattttaggtgttgccttaattttgcattacacttatttg-cagCTTGTGTTAAGCACTGGAACTGGTGTTAATGGATTTACCCTTGATCCATCACTTGGGGAGTTCATACTTACTCACCCAAACATTAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|29670800|gb|CB667075.1|CB667075

EST:     GAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACG                         CTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCC
genomic: GAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACGgtaaccatgt ... ggtatgacagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCC

EST: gi|29663073|gb|CB659348.1|CB659348

EST:     GAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACG                         CTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCC
genomic: GAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACGgtaaccatgt ... ggtatgacagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCC

EST: gi|29644325|gb|CB649332.1|CB649332

EST:     GAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACG                         CTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCC
genomic: GAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACGgtaaccatgt ... ggtatgacagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCC

EST: gi|33684972|gb|CF313211.1|CF313211

EST:     GAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACG                         CTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCC
genomic: GAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACGgtaaccatgt ... ggtatgacagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCC

EST: gi|29656833|gb|CB653108.1|CB653108

EST:     GAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACG                         CTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCCTTC
genomic: GAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACGgtaaccatgt ... ggtatgacagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCC-TTC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 59 (upstream exon), 74 (downstream exon)
Score: 46

CATGATTAAAGACAAGGACAACGTGACTTTGGACGATGTACTGCAACCTGGAACAGACATGCTTGCTGCTGGCTACTGCATGTACGGGAGTTCATGCACGgtaaccatgt ... ggtatgacagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCCCTTGGGGAGTTCATTCTGACTCATCCAAACATCAAG

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atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttcagatatatcacatgtactctcttcctaaatagacttggttctggtatgacagCTTGTGTTGAGCACTGGAAATGGAGTAAACGGTTTCACCCTTGATCCTTCCCTTGGGGAGTTCATTCTGACTCATCCAAACATCAAG