ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtgtgtttgccctcctatgattataaaataacctatcaaaattgccatggccttacttctgtaaattacttgcgtatagaaagatagcaactgttatgctttatttactgtttttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTTGTCAAAATAGCCAAG

Basic information

species	Oryza sativa
transcript	LOC_Os03g10940.1
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os03g10940.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA17G17790.1 (Glycine max), 3'ss of exon 5

----gtgtgtttgccctc--ctatgattataaaataacctatcaaaattgccatggccttacttc-tgtaaatt----------acttgcgtatagaaagatagcaactgttatgctttatttactgtttttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTTGTCAAAATAGCCAAG
    |  |  | ||  |   |||  | |      | |  | |  | ||  || |||| ||| ||  | | || |          |  | | | || |||       | |  |  | | |  | | | |  || ||||||||||||||||||||||| || || ||||| ||||| |||||||| ||||||||||||||||| |||
gtgagaattatagctataggctacaacttactggttatatgt-gacatacccttggcgttatttaatatgaagtctaattttgaatctacatgtacaaaatcgtgtaattctgggttatgatcattttacttaatgcagATATTTCGCAAGGAACCTTTTTTTTATGGTCACGACAACCATGATCAGCTTGTCAAAATAGCTAAG

upper sequence: LOC_Os03g10940.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA11G05340.2 (Glycine max), 3'ss of exon 5

---------gtgtgtttgccctcctatgattataaaataacctatcaaaatt---gccatggccttacttctgtaaattacttgcgtatagaaagatagcaactgttatgctttatttactgt-ttttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTTGTCAAAATAGCCAAG
          |   | |    | ||     ||||    |  || |  | |||   ||    |   ||  | || |   ||| ||  || | |   | |      || ||  ||| ||   |||  | |  || ||||||||||||| ||||| ||||| ||||| || || |||||||| ||||||||||||||||| |||
gtgtgaattataactataagttactggttatatatggcatactcttgacatttatgcagatggtctaaatttg-agcgtacatgattaaaaatgtaaaattctggtcataatttgttcgttgtactcttttgtagATATTTCGCAAAGAACCTTTCTTTTATGGTCATGACAACCATGATCAGCTTGTCAAAATAGCTAAG

upper sequence: LOC_Os03g10940.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA05G22250.1 (Glycine max), 3'ss of exon 5

---------------gtgtgtt-tgccctcctatgattataaaataacctatcaaaattgccatggccttacttctgtaaattact-tgcgtatagaaagatagcaactgttatgctttatttactgtttttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTTGTCAAAATAGCCAAG
                |  | |    | | ||     |||   | |  || |    |||    ||   | |  |||     | | | | | | || ||| || |      |   | |||  | |   |  || ||| ||||||||||||||||||| ||||| ||||| ||||| |||||||||||||||||||||||||| |||
gtgagaattatagctataggctacaacttactggttatatgtgacatgctcttggcattatt-tgatatgaagtctaattttgaatctacatgtacaaa-atcgtgtaattctgggtttgatcatcttacttcatgtagATATTTCGCAAGGAACCTTTCTTTTATGGTCACGACAACCATGACCAGCTTGTCAAAATAGCTAAG

upper sequence: LOC_Os03g10940.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA01G39950.1 (Glycine max), 3'ss of exon 5

-------gtgtgtttgccctcctatgattataaa--ataacctatcaaaattgccatggccttacttctgtaaattacttgcgtatagaaagatagcaactgttatgctttatttactgtttttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTTGTCAAAATAGCCAAG
          |   | |     | |||||||| |    |  || |  | |||      |     ||    |  |  |     |  || |||  |   |            | || | |||  ||  || ||||||||||||| ||||| ||||| ||||| || || |||||||| ||||||||||||||||| |||
gtgtgaatcataactacaagtttctgattatatatggcatactcttgacatttatgcagatggtctaaatttgagcatacatgattaaaaatgtgagattctggtcataattttcattgtactttttgtagATATTTCGCAAAGAACCTTTCTTTTATGGTCATGACAACCATGATCAGCTTGTCAAAATAGCTAAG

upper sequence: LOC_Os03g10940.1 (Oryza sativa), 3'ss of exon 5
lower sequence: Vv18s0001g09320.t01 (Vitis vinifera), 3'ss of exon 5

gtgtgtttgccctcctatgattataa-aataacctatcaaaattgccatggccttacttctgtaaatt--acttg-cgtatagaaagatagcaactgtta--tgctttattta--ctgtttttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTTGTCAAAATAGCCAAG
                |   ||| |     |     |   ||||  | |   | || | ||  ||||     ||| | |||  | | || | |||| | |  | ||  ||  |   | ||||     ||||| ||||||||||| |||||||| ||||| || ||||| |||||||||||||| ||||| ||||||
-------------gtaacagttacagtggcagttggtgttaattttcttttacatattacttgaaatgcagtttgtcttatcaataaat-gtaactttcaactactcaatggaggtttttttcccaacagATTTTTCGCAAGGAGCCATTCTTTTATGGTCATGATAATCATGACCAGCTTGTAAAAATTGCCAAG

upper sequence: LOC_Os03g10940.1 (Oryza sativa), 3'ss of exon 5
lower sequence: Vv07s0129g00410.t01 (Vitis vinifera), 3'ss of exon 5

gtgtgtttgccctcctatgattataaaataacctatcaaaattgccatggccttacttctgtaaattacttgcgtatagaaagatagcaactgttatgctttat-ttactgtttttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTTGTCAAAATAGCCAAG
||| | ||   || |||   ||       || |  |  ||      || |    | ||   |   ||   |   | |  |     |     | |||  |  |||  | || || || ||| |||| |||||||||||||||||||| ||||| || || || ||||| ||||||||||| || ||||||
gtgagatt-tactactactgttgatcttgaatcatttgaagggctgattgtgccatttacattttttgtatttattttaattatcattttgttttactcagtatacttctattatttatgtagATCTTTCGCAAGGAACCATTCTTTTATGGTCATGACAATCATGATCAGCTTGTCAAGATTGCCAAG

upper sequence: LOC_Os03g10940.1 (Oryza sativa), 3'ss of exon 5
lower sequence: EFJ20478 (Selaginella moellendorffii), 3'ss of exon 5

gtgtgtttgccctcctatgattataaaataacctatcaaaattgccatggccttacttctgtaaattacttgcgtatagaaagatagcaactgttatgctttatttactgtttttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTTGTCAAAATAGCCAAG
                                                            || | |   |||  |   ||    | | |  || ||      |    |     || |  |||||||||||||| ||||||||||| |||||||| || || || || ||||| || || || ||||||
------------------------------------------------------------gtgagtcttttgtct---gatcattcgtacttgcta------acgccccaagcttca--cagATATTTCGCAAAGAACCATTCTTTTATGGCCATGACAATCACGATCAGCTGGTGAAGATTGCCAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|87016659|gb|CI275661.1|CI275661

EST:     ATGACTACTCTTTAGACATGTGGAGCCTTGGCTGTATGTTTGCTGGAATG                         ATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTT
genomic: ATGACTACTCTTTAGACATGTGGAGCCTTGGCTGTATGTTTGCTGGAATGgtgtgtttgc ... tttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTT

EST: gi|66923472|gb|CX110320.1|CX110320

EST:     ATGACTACTCTTTAGACATGTGGAGCCTTGGCTGTATGTTTGCTGGAATG                         ATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTT
genomic: ATGACTACTCTTTAGACATGTGGAGCCTTGGCTGTATGTTTGCTGGAATGgtgtgtttgc ... tttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTT

EST: gi|25806912|gb|CA762873.1|CA762873

EST:     ATGACTACTCTTTAGACATGTGGAGCCTTGGCTGTATGTTTGCTGGAATG                         ATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTT
genomic: ATGACTACTCTTTAGACATGTGGAGCCTTGGCTGTATGTTTGCTGGAATGgtgtgtttgc ... tttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTT

EST: gi|86261473|gb|CI017215.1|CI017215

EST:     TATGTTTGCTGGAATG                         ATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTT
genomic: TATGTTTGCTGGAATGgtgtgtttgc ... tttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 66 (upstream exon), 65 (downstream exon)
Score: 15

ATACTTCAAAGGGCCTGAATTGCTGGTTGACTTCCAAAGTTATGACTACTCTTTAGACATGTGGAGCCTTGGCTGTATGTTTGCTGGAATGgtgtgtttgc ... tttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTTGTCAAAATAGCCAAG

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atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

acttgcgtatagaaagatagcaactgttatgctttatttactgtttttgatgcagATATTTCGCAAGGAACCATTCTTCTATGGCCACGATAACCATGACCAGCTTGTCAAAATAGCCAAG