ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtaatgtaaattatcctacactgttagtgattttcatggtaggcactgaatacaatgatcactttgtaaattttatttttgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACTTGCGGACTTTGGCATGGCTAAACAT

Basic information

species	Oryza sativa
transcript	LOC_Os02g35010.1
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os02g35010.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GRMZM2G540772_T01 (Zea mays), 3'ss of exon 6

gtaatgtaaattatcctacactgttagtgattttcatggtaggcactgaatacaatgatcactttgtaaattt----tatttttgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACTTGCGGACTTTGGCATGGCTAAACAT
||| ||| ||| ||   ||||| ||  || |||  |  ||     |||||||  |  || | | |   | ||     |  |||  | | ||||||| | |||||||||||| ||||||| ||||| ||||| |||||||||||||| || ||||||||||||||||||
gtactgtgaatgatttcacactatttctg-tttctaaagttaaagctgaataacac-attagtataacacttcacaatgctttatttggcagAGACGTAAAAGGAGCAAACGTACTTGTTGGCCCAAATGGCGAAGTCAAACTTGCTGATTTTGGCATGGCTAAACAT

upper sequence: LOC_Os02g35010.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA05G32510.1 (Glycine max), 3'ss of exon 5

gtaatgtaaattatcctacactgttagtgattttcatggtaggcactgaatacaatgatcactttgtaaattttatttt-tgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACTTGCGGACTTTGGCATGGCTAAACAT
||| | | | |  |||||| |  |   | |  |      |  |   |   ||   |  |||  |||| |   |  |||| | | | ||| || |||||||| || |||||||| || |  |||||||||||| ||||||| || |||||||| ||||||||||||
gtattttgattattcctacgcaatctttaacgtg-gcatttagtcttatttattctt-tcatattgtcatcgtggttttcttttggcagGGATATTAAAGGGGCGAACATACTAGTTGATCCTAATGGTGAAATCAAACTGGCAGACTTTGGAATGGCTAAACAT

upper sequence: LOC_Os02g35010.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA06G15870.1 (Glycine max), 3'ss of exon 6

gtaatgtaaattatcctacactgtt-agtgattttcatggtaggcactgaatacaatgatcactttgtaaattttatttt-tgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACTTGCGGACTTTGGCATGGCTAAACAT
||||| |||||       ||   || | | || || |  | | |||          | || |   | |  ||    |||| | |  |||| || || ||||| || |||||||| || |  |||||||||||| |||| || || |||||||| ||||||||||||
gtaatttaaatggctactcaagatttatttatgttaaatgcaagcatcttgactctttattatagtattgataagtttttattttcacagGGATATCAAAGGGGCTAACATACTAGTTGATCCTAATGGTGAAATCAAGCTGGCAGACTTTGGAATGGCTAAACAT

upper sequence: LOC_Os02g35010.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA08G16670.2 (Glycine max), 3'ss of exon 5

gtaatgtaaattatcctacactgttagtgattttcatggtaggcactgaatacaatgat-cactttgtaaattttatttt-tgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACTTGCGGACTTTGGCATGGCTAAACAT
||| | | | |  |||||| |  |   | |  |  |   |||||  |   ||   |  | ||  |||| |   |  |||| | | | ||| || |||||||| || |||||||| || |  |||||||||||| ||||||| || |||||||| ||||||||||||
gtattttgattattcctacgcaatctttaacatgaaatttaggc-ttatttattctctttcatattgttatcgtggttttcttttggcagGGATATTAAAGGGGCGAACATACTAGTTGATCCTAATGGTGAAATCAAACTGGCAGACTTTGGAATGGCTAAACAT

upper sequence: LOC_Os02g35010.1 (Oryza sativa), 3'ss of exon 5
lower sequence: GLYMA04G39110.1 (Glycine max), 3'ss of exon 5

gtaatgtaaattatcctaca----ctgttagtgattttcatggtaggcactgaatacaatgatcactttgtaaattttatttt-tgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACTTGCGGACTTTGGCATGGCTAAACAT
||||| ||||| ||    ||     | ||  | |   | |  | | |||          | || |   | |  ||    |||| | |  |||| || || ||||| || |||||||| || |  |||||||||||| |||| || || |||||||| ||||||||||||
gtaatttaaataattactcaagatttatttataacactaaatgcatgcatcttgactctttattatagtattgataagtttttattttcacagGGATATCAAAGGGGCTAACATACTAGTTGATCCTAATGGTGAAATCAAGCTGGCAGACTTTGGAATGGCTAAACAT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|218478760|gb|FG954799.1|FG954799

EST:     CAGATTCTTTCAGGGCTTGCCTACTTGCATGGTAGGAATACTGTACACAG                         AGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACT
genomic: CAGATTCTTTCAGGGCTTGCCTACTTGCATGGTAGGAATACTGTACACAGgtaatgtaaa ... ttgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACT

EST: gi|218486188|gb|FG948835.1|FG948835

EST:     GATTCTTTCA-GGCTTGCCTACTTGCATGGTAGGAATACTGTACACAG                         AGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACT
genomic: GATTCTTTCAGGGCTTGCCTACTTGCATGGTAGGAATACTGTACACAGgtaatgtaaa ... ttgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACT

EST: gi|38468550|gb|CF952681.1|CF952681

EST:     TTTCAGGGC-TGCCTACTTGCATGGTAGGAATACTGTACACAG                         AGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACT
genomic: TTTCAGGGCTTGCCTACTTGCATGGTAGGAATACTGTACACAGgtaatgtaaa ... ttgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 60 (upstream exon), 64 (downstream exon)
Score: 10

TTAGAGAGTACGGTCCATTTAAGGAACCTGTGATTCGTAATTATACTCGGCAGATTCTTTCAGGGCTTGCCTACTTGCATGGTAGGAATACTGTACACAGgtaatgtaaa ... ttgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACTTGCGGACTTTGGCATGGCTAAACAT

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atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tcatggtaggcactgaatacaatgatcactttgtaaattttatttttgtggacagAGACATTAAAGGAGCAAACATACTTGTGGGCCCTAATGGTGAAGTCAAACTTGCGGACTTTGGCATGGCTAAACAT